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1.
Audrey G. Quentin Elizabeth A. Pinkard Christopher L. Beadle Timothy J. Wardlaw Anthony P. O’Grady Stephen Paterson Caroline L. Mohammed 《Annals of Forest Science》2010,67(2):203-203
2.
大丽轮枝菌毒素诱导表达陆地棉cDNA序列的克隆与定位 总被引:3,自引:0,他引:3
为获得陆地棉黄萎病抗性相关候选基因,利用棉花黄萎病菌大丽轮枝菌V991毒素粗提物诱导耐黄萎病陆地棉品种'中棉12'应激基因的表达。在诱导幼根24 h时间点提取总RNA,PCR法合成双链cDNA,应用抑制消减杂交技术[1],得到了差异表达的180个克隆。经反Northern blot筛选出15个受毒素粗提物诱导应激表达的阳性cDNA克隆,将其命名为Vdrg(response gene induced by toxin of Verticillium dahliae)。本研究对所得阳性cDNA克隆进行了序列分析,并利用Pima S6 与 Acala Maxxa 海陆杂交四倍体棉花F2群体对Vdrg序列进行了棉花基因组定位。 相似文献
3.
The growing set of fully-sequenced angiosperm genomes highlight the role of polyploidy in angiosperm evolution suggest that even the high level of importance we had already attributed to this mechanism was inadequate.…… 《分子植物育种》2007,5(2):151
The growing set of fully-sequenced angiosperm genomes highlight the role of polyploidy in angiosperm evolution, and suggest that even the high level of importance we had already attributed to this mechanism was inadequate. 相似文献
4.
Landscape Ecology - Habitat loss and fragmentation contribute significantly to pollinator decline and biodiversity loss globally. Conserving high quality habitats whilst restoring and connecting... 相似文献
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6.
Objective To determine the effectiveness of a topical anaesthetic formulation (Tri-Solfen) with or without the administration of a non-steroidal anti-inflammatory drug (carprofen) on the pain and distress response associated with ring or surgical castration of ram lambs.
Procedures Merino ram lambs (n = 78) were allocated to 10 treatment groups: 4 groups of knife-castrated lambs and 4 groups of ring-castrated lambs received carprofen (4 mg/kg SC) and Tri-Solfen; 2 control groups (sham) received carprofen at 0 or 4 mg/kg SC. Measurements included plasma cortisol and haptoglobin concentrations, haematology, and behaviour, including posture.
Results Knife-castrated lambs had higher peak cortisol and integrated cortisol responses for the first 6 h after treatment and greater concentration s of circulating acute phase proteins than ring-castrated lambs, both of which were significantly different from the sham controls. Tri-Solfen applied to the knife castration wound significantly reduced both the peak plasma cortisol concentration and the integrated cortisol response for the first 6 h and improved lying behaviour in the first 12 h. Carprofen reduced the cortisol response to knife castration at 30 min, but elevated the cortisol responses at 24 and 48 h. Carprofen nearly halved the number of acute pain behaviours associated with ring castration. There were no significant additive or synergistic effects from combining the analgesic treatments. Tri-Solfen applied to the tail wound provided no detectible benefits during ring castration + tail docking.
Conclusions The physiological and behavioural responses suggest that ring castration has less impact on the lamb than knife castration. The specific analgesic treatments can provide modest amelioration of the pain and discomfort associated with castration. Alternative doses or application methods may enhance their efficacy. 相似文献
Procedures Merino ram lambs (n = 78) were allocated to 10 treatment groups: 4 groups of knife-castrated lambs and 4 groups of ring-castrated lambs received carprofen (4 mg/kg SC) and Tri-Solfen; 2 control groups (sham) received carprofen at 0 or 4 mg/kg SC. Measurements included plasma cortisol and haptoglobin concentrations, haematology, and behaviour, including posture.
Results Knife-castrated lambs had higher peak cortisol and integrated cortisol responses for the first 6 h after treatment and greater concentration s of circulating acute phase proteins than ring-castrated lambs, both of which were significantly different from the sham controls. Tri-Solfen applied to the knife castration wound significantly reduced both the peak plasma cortisol concentration and the integrated cortisol response for the first 6 h and improved lying behaviour in the first 12 h. Carprofen reduced the cortisol response to knife castration at 30 min, but elevated the cortisol responses at 24 and 48 h. Carprofen nearly halved the number of acute pain behaviours associated with ring castration. There were no significant additive or synergistic effects from combining the analgesic treatments. Tri-Solfen applied to the tail wound provided no detectible benefits during ring castration + tail docking.
Conclusions The physiological and behavioural responses suggest that ring castration has less impact on the lamb than knife castration. The specific analgesic treatments can provide modest amelioration of the pain and discomfort associated with castration. Alternative doses or application methods may enhance their efficacy. 相似文献
7.
8.
In accordance with the EPPO guideline for the efficacy evaluation of plant protection products, resistance risk analysis PP 1/213(1), a method was established to determine the baseline sensitivity of key weed species to florasulam, a new triazolopyrimidine sulfonanilide herbicide for post-emergence control of dicotyledonous weeds in cereals. The aim of the baseline monitoring project was to understand the natural variation in response to florasulam of diverse populations of Papaver rhoeas at the time of product launch. The method entailed seed collection from representative agricultural areas throughout Europe. The seed was subjected to glasshouse tests where dose-response studies were conducted and ED80 values generated. This enabled a sensitivity index to be calculated for each country, giving an indication of the variation in P rhoeas response to florasulam in the populations tested. 相似文献
9.
David L Mann Tom Asakawa Beverley Kelly Trent Lindsay & Brian Paterson 《Aquaculture Research》2007,38(14):1580-1587
Intensive nursery systems are designed to culture mud crab postlarvae through a critical phase in preparation for stocking into growout systems. This study investigated the influence of stocking density and provision of artificial habitat on the yield of a cage culture system. For each of three batches of postlarvae, survival, growth and claw loss were assessed after each of three nursery phases ending at crab instars C1/C2, C4/C5 and C7/C8. Survival through the first phase was highly variable among batches with a maximum survival of 80% from megalops to a mean crab instar of 1.5. Stocking density between 625 and 2300 m−2 did not influence survival or growth in this first phase. Stocking densities tested in phases 2 and 3 were 62.5, 125 and 250 m−2. At the end of phases 2 and 3, there were five instar stages present, representing a more than 20‐fold size disparity within the populations. Survival became increasingly density‐sensitive following the first phase, with higher densities resulting in significantly lower survival (phase 2: 63% vs. 79%; phase 3: 57% vs. 64%). The addition of artificial habitat in the form of pleated netting significantly improved survival at all densities. The mean instar attained by the end of phase 2 was significantly larger at a lower stocking density and without artificial habitat. No significant effect of density or habitat on harvest size was detected in phase 3. The highest incidence of claw loss was 36% but was reduced by lowering stocking densities and addition of habitat. For intensive commercial production, yield can be significantly increased by addition of a simple net structure but rapidly decreases the longer crablets remain in the nursery. 相似文献
10.
R. T. Paterson G. M. Karanja O. Z. Nyaata I. W. Kariuki R. L. Roothaert 《Agroforestry Systems》1998,41(2):181-199
Although the biological advantages in terms of animal production and improved soil fertility of the use of herbaceous legumes
have been well demonstrated in Kenya and elsewhere, adoption by small-scale farmers has often been disappointing. This has
led to increased research into the use of both indigenous and exotic fodder trees. In common with conventional pasture legumes,
tree fodders contain high levels of crude protein and minerals and many show high levels of digestibility. They are readily
accepted by livestock and presumably because of their deep-root systems, they continue to produce well into the dry season.
Antinutritive factors can be a problem, however, and polyphenolics, toxic amino acids, cyanogenic glycosides and alkaloids
are found in many tree species. There are abundant niches on small farms where fodder trees can be grown without affecting
crop production. Although detailed management recommendations are lacking, guidelines exist with regard to appropriate cutting
heights and harvesting frequencies. In the Embu region, it has been estimated that three kg of fresh fodder of Calliandra
calothyrsus has the same effect on milk production as one kg of commercial dairy meal. Up to about 500 trees (250 m of hedgerow)
will produce enough fodder to supplement one dairy cow for a complete lactation. The tree fodder can either replace the concentrate
without loss of yield, or it can complement it to produce more milk. It is being enthusiastically adopted by small-scale farmers,
many of whom are starting to produce their own seed. Other tree species are now being studied in order to avoid over-reliance
on a single fodder species.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献