Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.     

高职食品与药品类专业“基础化学”教学改革与实践

“基础化学”理论和实验教学质量的“高与低”在一定程度上直接关乎高职院校与化学相近专业人才培养的“成与败”。以教学改革为切入点,在“基础化学”教材建设和系列微课的制作及应用2个维度上,探讨提升“基础化学”课程教学质量的具体措施。     

Studies on Tomato Seedling Quality Indices Under Simulated Shipping and Storage Conditions

Indices of the tomato seedling quality maintenance level after production before field planting were studied through simulated experiments, small--scale operation, indoor analyses and measurements, and field observation. The results showed that under simulated shipping and storage conditions, seedling quality change following different durations (days) of shipping and storage was correlated significantly or even very significantly with certain physiological and morphological indices. With various measured indices following different periods of shipping and storage treatment subjected to multinomial successive regressive correlation analysis, the principal factors influencing seedling quality maintenance level are identified to be chlorophyll content→dry short weight→ leaf freshness index in order of their importance. Significance analysis with multinomial fitted equation indicated that correlations between any one of above three factors and the growth index after field planting reached very significant difference level.     

云南省宜良县大型真菌调查(续)

94 .黄白粘盖牛肝菌Suillusplacidus (Bonorder)Sing .(食、毒 )95 .亚金黄粘盖牛肝菌S .subaureus (Perk)Snell(食 ?)96 .白短柄粘盖牛肝菌S .albidipes (Peck)Sing .(食 ?)97.腺点柄粘盖牛肝菌S .landulospesA .H .SmithetThiers (食 ?)98.黄粘盖牛肝菌S .lavidus (Fr .)Sing .(食 ?)99.暗黄粘盖牛肝菌S .plorans (Roll)Sing .(食 ?)10 0 .黑盖粉孢牛肝菌Tylopilusalboater (Schw .)Murr.(食 )10 1.白粉孢牛肝菌T .albofarinaceus (Chiu)Tai(食 )10 2 .锈盖粉孢牛肝菌T .ballouii (Peck)Sing .(食 ?)10 3.紫盖粉孢牛肝菌T .ex…     

关中地区几种苜蓿地上生物量初步研究

对5个苜蓿Medicago sativa品种的地上生物量形成规律进行了初步研究.结果表明:苜蓿生物量的增长速率不仅与本身生理特性有关,而且与环境密切相关;株高在分枝末期现蕾初期增长速度快.绝对生长速率与相对生长速率在分枝末期现蕾期都较大,此时的水分管理具有重要意义;不同苜蓿品种的群落结构大多为菱形;同化系统与非同化系统的比值随高度的增加而减少.     

浊度法测定维生素C注射液细菌内毒素含量

应用BET-32型细菌内毒素测定仪定量测定维生素C注射液的细菌内毒素含量，干扰试验表明，维生素C注射液在400倍稀释时，测定内毒素的干扰最小。7个不同批号的维生素C注射液测定结果显示，7批的回收率均符合测定要求，其中4个批号的人用维生素C注射液内毒素含量符合药典标准，兽用3个批号产品的内毒素含量偏高。     

东方蝾螈外部体型指数回归方程的建立

为了探讨东方蝾螈在人工养殖效果评估方面 ,在确定屠宰或捕捞的大小与时间的适宜度方面 ,在生理、病理分析时 ,在治疗时间和用药量等诸方面 ,都达到取材时期的合理性问题 ,测定了东方蝾螈的身型指数 ,并初步建立了回归方程Y =- 2 5 0 +0 .6 2X1 ,并对相关数据的处理方法进行了探讨。     

不同温度、不同时间处理对打破长白楤木芽休眠的影响

长白楤木，又名草本龙芽、东北土当归、狗苦龙芽、中尾大活等，为五加科的大型草本植物。其嫩芽可作产品器官食用，味美、有保健作用，是很有发展前景的野菜种类。     

摄入不同能量的围产期乳牛肝低密度脂蛋白受体mRNA丰度的比较

将30头健康、经产、处于围产期的黑白花乳牛随机分为3组，每组10头。从产前28d开始，低能量组乳牛饲喂《中国奶牛饲养标准（2000）》减少20％日粮（能量摄入80％），对照组乳牛饲喂《标准》日粮（能量摄入100％），高能量组乳牛饲喂《标准》增加20％日粮（能量摄入120％），产后各组乳牛均饲喂标准日粮。至产后第56d结束试验；采用内对照RT-PCR方法检测摄入不同能量的围产期乳牛肝活体组织低密度脂蛋白受体（LDLR）mRNA丰度。结果，不同能量组乳牛肝LDLR mRNA丰度产前至产后均呈现先升高后降低的趋势。100％和120％能量组肝LDLR mRNA丰度在产后14d达最大值，且产后均高于产前（产后56d除外，P〈0．01或P〈0．05）；而80％能量组产后1d即达到最大值，产前14d至产后14d，LDLR mRNA相对表达量显著高于100％和120％能量组；产后28～56d，120％能量组显著高于80％和100％能量组（P〈0．01）。表明围产期乳牛能量摄入水平对肝LDLR mRNA丰度有显著影响。     

鸡传染性腔上囊病病毒VP2基因在昆虫细胞中的表达

为了深入研究鸡传染性腔上囊病病毒（IBDV）VP2基因的结构和功能,利用Bac-to-Bac系统研制出含有VP2基因的重组杆状病毒rBac-VP2,将rBac-VP2感染Sf9细胞,并用抗IBDV VP2特异性单克隆抗体经间接免疫荧光试验检测,证实感染重组病毒Sf9细胞能高效表达IBDV VP2基因产物;Western-blotting分析结果表明,VP2基因表达产物的分子质量约为40 ku.