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JI Yun HJ Park MH Park MS Kim JH Choi E Lee SP Gong JM Lim ST Lee 《Reproduction in domestic animals》2014,49(5):705-710
Recently, isolation and in vitro culture of putative spermatogonial stem cells (SSCs) in the domestic cat have been conducted. However, the cellular niche conditions that facilitate the establishment and long‐term maintenance of feline SSCs (FSSCs) have not been described. Therefore, we investigated the type of feeder cells used to stimulate colony formation and growth of FSSCs among the various factors in the FSSC niche. Spermatogonial stem cells isolated from feline testes were cultured on mitotically inactivated testicular stromal cells (TSCs) derived from cats, dogs and mice, and mouse embryonic fibroblasts (MEFs). The formation and growth of colonies derived from SSCs cultured on each type of feeder cell were identified at passage 0, and the morphology, alkaline phosphatase (AP) activity and expression of SSC‐specific genes in surviving colonies were investigated at passage 4. Among these diverse feeder cells, TSCs from cat showed the greatest colony formation, growth and maintenance of FSSCs, and SSC colonies cultured by passage 4 showed a typical dome‐shaped morphology, AP activity and expression of SSC‐specific genes (NANOG, OCT4, SOX2 and CD9). Accordingly, these results demonstrate that feline TSCs could be used as feeder cells to support the establishment and maintenance of SSCs from domestic cats. 相似文献
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THE ISOLATION OF A ROSS RIVER VIRUS FROM A HORSE 总被引:1,自引:0,他引:1
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Five diets each calculated to contain about 3.0 kcal ME/g, with determined crude protein levels of 17.5, 19.8, 21.7, 23.2 and 24.4% were fed to male and female turkeys from 8 to 12 weeks of age. The 23.2 and 24.4% protein diets were estimated to contain about 115 and 125% respectively of the lysine and methionine allowances recommended for maximum growth at this stage. But for both sexes growth increments increased progressively as dietary protein increased up to the highest level fed, indicating that protein requirements for maximum growth were in this case considerably in excess of currently recommended levels.
There was no consistent effect of sex or diet on visual assessment of carcass finish or conformation at 12 weeks of age. “Skin‐pinch” thickness was significantly greater for females than for males. In the case of females there was a tendency for skin‐pinch thickness to decrease with increasing dietary protein level and body weight. 相似文献
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