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排序方式: 共有1313条查询结果,搜索用时 31 毫秒
1.
Mouse pronuclear stage embryos with intact slit zona pellucida (manipulated) were cultured in vitro until the hatched blastocyst stage in simplex optimized medium with higher K+ concentration (KSOM) prepared with three different water types: tap, deionized reverse osmosis (D‐O) water and Milli‐Q system (M‐Q) water. The culture media were supplemented with or without protein and ethylenediaminetetraacetic acid (EDTA, disodium salt). The rates of hatched blastocysts were significantly affected (p < 0.01) by micromanipulation, protein supplement and water source. The water source has no influence (p > 0.05) on development in EDTA‐supplemented protein‐free culture media, whereas in EDTA‐ and protein‐free culture media, the water quality significantly (p < 0.001) affected the rates of development, with higher rates in media prepared with M‐Q water. The micromanipulated embryos showed higher sensitivity to the water quality (p < 0.01). It worth mentioning that the rates of hatched blastocysts in protein‐free culture media were very low (0–7.5%). Furthermore, the three different water types were analysed by measuring the electrical conductivity, inorganic ions, total organic carbon and endotoxins to evaluate the purity. M‐Q water showed the lowest levels of inorganic ion, total organic carbon and endotoxin concentrations. We concluded that manipulated mouse embryos are good system to evaluate the quality of water used in biological system. 相似文献
2.
A ten-year-old female poodle was presented for left hindlimb lameness. Radiographically, irregular intramedullary densities of long bones (bone infarcts) and a lytic lesion in the left distal femur were detected. Two years after the first examination, the lesions in the distal femur were definitely identified as osteosarcoma and the dog was euthanized. Pathological and histological changes of the bone infarcts as well as of the osteosarcoma are described. Regarding bibliographical data, occurrence and aetiopathogenesis of osteosarcoma associated with multifocal bone infarction in man and dog are discussed. 相似文献
3.
In order to investigate usefulness of electrodiagnostic methods in Cauda Equina-Compression, 26 dogs with this disease were subjected to electromyography and spinal evoked potentials. 16 normal dogs served as controls. It was found that--after stimulation of the Nn.tibialis, peronaeus, pudendus and coccygeales and recording at the lumbosacral junction--latencies and nerve-conduction velocities were largely normal. However the amplitudes were decreased and--depending on the severity of the compression--the morphology of the potentials was altered although considerable standard deviations were obtained. Electromyographically, increased spontaneous activity with fibrillations and positive sharp waves were found in the myotomes of the lumbosacral nerves. Their number increased according to the severity of the cauda equina compression and they had a centrifugal distribution pattern. Based on these results it appears indicated to perform an electromyographic exam before applying myelography. The technique of spinal evoked potentials will need more refinement and standardisation before being useful in a clinical situation. 相似文献
4.
Hao Wenkang Lang Kuijian Zhang Yanli Li Longchang Weng Guoqing Huang Mingquan Wang Xiangguo 《林业研究》1993,4(2):33-42
Site quality of larch (Larix gmelinii Rupr.) in Da Xingan Mountain in Inner Mongolia was studied systematically with about 1000 temporary plots and computer software.
The nature of site class and site index curve was discussed in view of general mathematical model of height growth. The height
growth of dominant tree of four divisions of forest stands were studied and site index tables were constructed respectively.
In order to unify the comparing standard and convenience for management in this region, site index table was constucted too.
Based on discussing the action and accuracy of forest type, a series of site quality evaluation (forest type-site index class-site
index) was suggested.
This subject of study is supported by the National Natural Scientific Fund (No. 39270549). 相似文献
5.
非公有制林业主体融资问题的思考 总被引:4,自引:0,他引:4
针对不同层次的个体林业,设计了民间融资、"报账制"式银行信贷等方式以求解决个体林农融资困难问题. 相似文献
6.
7.
ACE inhibition in goats under fixed‐time artificial insemination protocol increases the pregnancy rate and twin births
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VP Fernandes Neto MNN Silva AS Costa D Kunkel AS Júnior LCS Feitosa MCS Muratori APR Costa 《Reproduction in domestic animals》2018,53(4):1006-1008
To assess the effect of the angiotensin‐converting enzyme (ACE) inhibition on the efficiency of the fixed‐time artificial insemination (TAI), 69 goats were divided randomly into two groups: enalapril (n = 35) and control (n = 34). In the experiment, all animals underwent the protocol of fixed‐time artificial insemination for 12 days. Enalapril group received enalapril maleate dissolved in saline (Enalapril, Lab Teuto Ltda) subcutaneously at the following doses: 0.2 mg/kg/day in D0‐D2; 0.3 mg/kg/day in D3‐D6 and 0.4 mg/kg/day in D7‐D11. The control group received the corresponding volume of 0.9% saline solution. We performed a single insemination 36 hr after sponge removal using frozen semen from two adult male goats with recognized fertility. The ultrasound pregnancy diagnosis was 30 days after the artificial insemination (AI). There was significant increase in pregnancy rates and twinning as well as a decrease in foetal loss in animals receiving enalapril (p < .01). The use of ACE inhibitors during the TAI protocol was shown to be a promising alternative to increase the efficiency of such reproductive biotechnology. 相似文献
8.
Previous studies showed that the yellow seed color gene of a yellow mustard was located on the A09 chromosome. In this study, the sequences of the molecular markers linked to the yellow seed color gene were analyzed, the gene was primarily mapped to an interval of 23.304 to 29.402M. Twenty genes and eight markers’ sequences in this region were selected to design the IP and SCAR primers. These primers were used to screen a BC8S1 population consisting of 1256 individuals. As a result, five IP and five SCAR markers were successfully developed. IP4 and Y1 were located on either side of the yellow seed color gene at a distance of 0.1 and 0.3 cM, respectively. IP1, IP2 and IP3 derived from Bra036827, Bra036828, Bra036829 separately, co-segregated with the target gene. BLAST analysis indicated that the sequences of newly developed markers showed good collinearity with those of the A09 chromosome, and that the target gene might exist between 27.079 and 27.616M. In light of annotations of the genes in this region, only Bra036828 is associated with flavonoid biosynthesis. This gene has high similarity with the TRANSPARENT TESTA6 gene, Bra036828 was hence identified as being the gene possibly responsible for yellow seed color, in our research. 相似文献
9.
番茄新品种长丰88是以CT15为母本,CT101-3为父本配制的抗TY、抗线虫杂交一代良种,属无限生长红果类型。生长势强,第7片叶着生第1花穗,每隔2片叶着生一花穗,中早熟。坐果率高、连续结果能力强,春季每穗平均结果 20个,产量59 850 kg/hm~2;果实圆形,无绿色果肩,表面光滑,外形美观。果实硬度大,耐贮运,货架寿命长,单果质量20 g左右,大小均匀。可溶性固形物含量8.9%,VC含量32.43 mg/100 g,总糖3.44%,总酸0.236%。抗病性强,抗番茄黄化曲叶病毒病,根结线虫病发病率为0,灰霉病和早疫病均未见发生。 相似文献
10.