Manipulated Mouse Embryos as Bioassay System for Water Quality Control

Mouse pronuclear stage embryos with intact slit zona pellucida (manipulated) were cultured in vitro until the hatched blastocyst stage in simplex optimized medium with higher K⁺ concentration (KSOM) prepared with three different water types: tap, deionized reverse osmosis (D‐O) water and Milli‐Q system (M‐Q) water. The culture media were supplemented with or without protein and ethylenediaminetetraacetic acid (EDTA, disodium salt). The rates of hatched blastocysts were significantly affected (p < 0.01) by micromanipulation, protein supplement and water source. The water source has no influence (p > 0.05) on development in EDTA‐supplemented protein‐free culture media, whereas in EDTA‐ and protein‐free culture media, the water quality significantly (p < 0.001) affected the rates of development, with higher rates in media prepared with M‐Q water. The micromanipulated embryos showed higher sensitivity to the water quality (p < 0.01). It worth mentioning that the rates of hatched blastocysts in protein‐free culture media were very low (0–7.5%). Furthermore, the three different water types were analysed by measuring the electrical conductivity, inorganic ions, total organic carbon and endotoxins to evaluate the purity. M‐Q water showed the lowest levels of inorganic ion, total organic carbon and endotoxin concentrations. We concluded that manipulated mouse embryos are good system to evaluate the quality of water used in biological system.     

Osteosarcoma of the distal femur, combined with multiple bone infarcts: the case of a 10-year-old poodle

A ten-year-old female poodle was presented for left hindlimb lameness. Radiographically, irregular intramedullary densities of long bones (bone infarcts) and a lytic lesion in the left distal femur were detected. Two years after the first examination, the lesions in the distal femur were definitely identified as osteosarcoma and the dog was euthanized. Pathological and histological changes of the bone infarcts as well as of the osteosarcoma are described. Regarding bibliographical data, occurrence and aetiopathogenesis of osteosarcoma associated with multifocal bone infarction in man and dog are discussed.     

Electromyography and spinal evoked potentials in cauda equina syndrome of dogs

In order to investigate usefulness of electrodiagnostic methods in Cauda Equina-Compression, 26 dogs with this disease were subjected to electromyography and spinal evoked potentials. 16 normal dogs served as controls. It was found that--after stimulation of the Nn.tibialis, peronaeus, pudendus and coccygeales and recording at the lumbosacral junction--latencies and nerve-conduction velocities were largely normal. However the amplitudes were decreased and--depending on the severity of the compression--the morphology of the potentials was altered although considerable standard deviations were obtained. Electromyographically, increased spontaneous activity with fibrillations and positive sharp waves were found in the myotomes of the lumbosacral nerves. Their number increased according to the severity of the cauda equina compression and they had a centrifugal distribution pattern. Based on these results it appears indicated to perform an electromyographic exam before applying myelography. The technique of spinal evoked potentials will need more refinement and standardisation before being useful in a clinical situation.     

称重式油脂添加设备的系统设计分析

<正>饲料生产中添加油脂是为了满足生产高浓度能量饲料的要求,同时还可减少氨基酸氧化,提高氨基酸的消化率;抑制葡萄糖和其它前体物质转化为脂肪;促进脂溶性维生素A、D、E、K和某些激素的吸收     

农业高校产学研合作创新的实践与对策建议

创新是一个民族进步的灵魂,是一个国家和地区兴旺发达的不竭动力。长期以来,农业高校积极利用自身人才与科技优势,主动面向经济建设,把学校优秀的科技成果、充分的人力资源、企业良好的市场运作、雄厚的资金结合起来,开展多种形式的产学研合作创新,推动了农业科技成果向现实生产力的转化,产生了积极的社会效益和经济效益。文章探讨了南京农业大学产学研合作创新的具体实践模式,提出了促进产学研有机结合,加快农业科技创新步伐的对策建议。