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Melatonin reduces apoptotic cells,SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts 下载免费PDF全文
TC Marques EC da Silva Santos TO Diesel LO Leme CF Martins MAN Dode BG Alves FPH Costa EB de Oliveira ML Gambarini 《Reproduction in domestic animals》2018,53(1):226-236
Effects of adding different concentrations of melatonin (10?7, 10?9 and 10?11 M) to maturation (Experiment 1; Control, IVM + 10?7, IVM + 10?9, IVM + 10?11) and culture media (Experiment 2; Control, IVC + 10?7, IVC + 10?9, IVC + 10?11) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10?9 M) from Experiments 1–2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM + 10?9, IVC + 10?9, IVM /IVC + 10?9). In Experiment 1, maturated oocytes from Control and IVM + 10?9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC + 10?7 (43.5%; 56.7%) and IVC + 10?9 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC + 10?9 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC + 10?9 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM /IVC + 10?9 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC + 10?9 treatment also had a higher mRNA expression of antioxidant gene (SOD 2) compared to the Control, as well as the heat shock protein (HSPB 1) compared to the IVM + 10?9. Reactive oxygen species production was greater in the IVM /IVC + 10?9 treatment group. In conclusion, the 10?9 M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality. 相似文献
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Quadratic fitting was used to regress semen characteristics of 1441 samples consisting of 12‐month collection from 58 Duroc boars against animal age varied from 10 to 80 months. Data was divided into two groups of cool (14.0–22.7°C, RH 81.5%) and hot season (22.9–29.9°C, RH 86.6%), to test effects of age, season and their interactions. Results revealed that young boars of around 1 year old could endure the hot season. The endurance gradually diminished as animals grew. In the hot season animals exhibited peak performance at age around 33 month and it remained for 1 month, while cool‐season kept boars could last for 48 months from 16 months old onward. The reproductive longevity should be 51 month in a subtropical environment and it may extend to 70 month if heat stress can be avoided. The estimated total sperm contribution of a Duroc boar would be 1.8 times more when kept below 22°C than in a natural subtropical environment. It is concluded that to maintain Duroc boars as semen donor to at least 4 years of age is feasible in a subtropical environment and boar longevity could reach 6 years old if well kept in a temperate region. 相似文献
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笋用竹害虫天敌资源研究 总被引:1,自引:0,他引:1
本文报道了浙江省笋用竹害虫天敌资源调查的结果,共查明天敌资源44种,经鉴定属于螳螂目1科2种,半翅目1科2种,蛇蛉目1科1种,脉翅目1科2种,鞘翅目2科9种,双翅目2科2种,膜翅目9科22种;蛛形纲蜱螨目1科1种,蜘蛛目3科3种,指出了天敌的寄主和分布。 相似文献
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Mei-Yee CHIN Sharon Yu Ling LAU Frazer MIDOT Mui Sie JEE Mei Lieng LO Faustina E. SANGOK Lulie MELLING 《土壤圈》2023,33(5):683-699
Soil respiration is a vital process in all terrestrial ecosystems, through which the soil releases carbon dioxide (CO2) into the atmosphere at an estimated annual rate of 68-101 Pg carbon, making it the second highest terrestrial contributor to carbon fluxes. Since soil respiration consists of autotrophic and heterotrophic constituents, methods for accurately determining the contribution of each constituent to the total soil respiration are critical for understanding their differential responses to environmental factors and aiding the reduction of CO2 emissions. Owing to its low cost and simplicity, the root exclusion (RE) technique, combined with manual chamber measurements, is frequently used in field studies of soil respiration partitioning. Nevertheless, RE treatments alter the soil environment, leading to potential bias in respiration measurements. This review aims to elucidate the current understanding of RE, i.e., trenching (Tr) and deep collar (DC) insertion techniques, by examining soil respiration partitioning studies performed in several ecosystems. Additionally, we discuss methodological considerations when using RE and the combinations of RE with stable isotopic and modeling approaches. Finally, future research directions for improving the Tr and DC insertion methods in RE are suggested. 相似文献