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1.
Kaiping Deng Steffen Uhlig Laura B. Goodman Hon S. Ip Mary Lea Killian Sarah M. Nemser Jodie Ulaszek Shannon Kiener Matthew Kmet Kirstin Frost Karina Hettwer Bertrand Colson Kapil Nichani Anja Schlierf Andriy Tkachenko Mothomang Mlalazi-Oyinloye Andrew Scott Ravinder Reddy Gregory H. Tyson 《Journal of veterinary diagnostic investigation》2022,34(5):825
The COVID-19 pandemic presents a continued public health challenge. Veterinary diagnostic laboratories in the United States use RT-rtPCR for animal testing, and many laboratories are certified for testing human samples; hence, ensuring that laboratories have sensitive and specific SARS-CoV2 testing methods is a critical component of the pandemic response. In 2020, the FDA Veterinary Laboratory Investigation and Response Network (Vet-LIRN) led an interlaboratory comparison (ILC1) to help laboratories evaluate their existing RT-rtPCR methods for detecting SARS-CoV2. All participating laboratories were able to detect the viral RNA spiked in buffer and PrimeStore molecular transport medium (MTM). With ILC2, Vet-LIRN extended ILC1 by evaluating analytical sensitivity and specificity of the methods used by participating laboratories to detect 3 SARS-CoV2 variants (B.1; B.1.1.7 [Alpha]; B.1.351 [Beta]) at various copy levels. We analyzed 57 sets of results from 45 laboratories qualitatively and quantitatively according to the principles of ISO 16140-2:2016. More than 95% of analysts detected the SARS-CoV2 RNA in MTM at ≥500 copies for all 3 variants. In addition, for nucleocapsid markers N1 and N2, 81% and 92% of the analysts detected ≤20 copies in the assays, respectively. The analytical specificity of the evaluated methods was >99%. Participating laboratories were able to assess their current method performance, identify possible limitations, and recognize method strengths as part of a continuous learning environment to support the critical need for the reliable diagnosis of COVID-19 in potentially infected animals and humans. 相似文献
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Pioneer 11 has made the first exploration of the magnetosphere and trapped radiation belts of Saturn. Saturn's magnetosphere is intermediate in size between Earth's and Jupiter's, with trapped particle intensities comparable to Earth's. The outer region of Saturn's magnetosphere contains lower energy radiation and is variable with time; the inner region contains higher energy particles. The pitch angle distributions show a remarkable variety of field-aligned and locally mirroring configurations. The moons and especially the rings of Saturn are effective absorbers of trapped particles; underneath the rings, the trapped radiation is completely absorbed. We confirm the discovery of a new ring, called the F ring, a new division, the Pioneer division, and a moon, called 1979 S 2. The latter has probably been seen from Earth. There may be evidence for more bodies like 1979 S 2, but at this stage the interpretation of the data is ambiguous. Using particle diffusion rates, we estimate that the cross-sectional area of the F ring is > 7 x 10(13) square centimeters and that the opacity is > 10(-5). Cosmic-ray albedo neutron decay should be looked into as a source of energetic particles in the inner magnetosphere of Saturn. 相似文献
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Sugita S Ootsubo T Kadono T Honda M Sako S Miyata T Sakon I Yamashita T Kawakita H Fujiwara H Fujiyoshi T Takato N Fuse T Watanabe J Furusho R Hasegawa S Kasuga T Sekiguchi T Kinoshita D Meech KJ Wooden DH Ip WH A'Hearn MF 《Science (New York, N.Y.)》2005,310(5746):274-278
The impact cratering process on a comet is controversial but holds the key for interpreting observations of the Deep Impact collision with comet 9P/Tempel 1. Mid-infrared data from the Cooled Mid-Infrared Camera and Spectrometer (COMICS) of the Subaru Telescope indicate that the large-scale dust plume ejected by the impact contained a large mass (approximately 10(6) kilograms) of dust and formed two wings approximately +/-45 degrees from the symmetric center, both consistent with gravity as the primary control on the impact and its immediate aftermath. The dust distribution in the inner part of the plume, however, is inconsistent with a pure gravity control and implies that evaporation and expansion of volatiles accelerated dust. 相似文献
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Dietary conjugated linoleic acid (CLA; 0-2.0%) increased CLA concentrations in liver microsomes and skeletal muscle homogenates from rats. Dietary CLA decreased oleic and arachadonic acid concentrations in both liver microsomes and skeletal muscle. The presence of CLA in liver microsomes had no impact on linoleic acid, arachadonic acid, and alpha-tocopherol oxidation rates. Dietary CLA (2.0%) also did not alter alpha-tocopherol oxidation rates in liver microsomes or muscle homogenates. Formation of malonaldehyde (MDA) in oxidizing liver microsomes decreased with increasing CLA concentration as determined by measurement of thiobarbituric acid-MDA complexes by HPLC. The ability of CLA to decrease MDA formation without impacting other lipid oxidation markers such as the disappearance of fatty acid and alpha-tocopherol suggests that decreased MDA concentration was the result of CLA's ability to lower polyenoic fatty acids such as arachadonic acid. While CLA does not appear to act as an antioxidant, its ability to decrease polyenoic fatty acid concentrations could decrease the formation of highly cytotoxic lipid oxidation products such as MDA. 相似文献
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Waite JH Cravens TE Ip WH Kasprzak WT Luhmann JG McNutt RL Niemann HB Yelle RV Mueller-Wodarg I Ledvina SA Scherer S 《Science (New York, N.Y.)》2005,307(5713):1260-1262
Ions were detected in the vicinity of Saturn's A ring by the Ion and Neutral Mass Spectrometer (INMS) instrument onboard the Cassini Orbiter during the spacecraft's passage over the rings. The INMS saw signatures of molecular and atomic oxygen ions and of protons, thus demonstrating the existence of an ionosphere associated with the A ring. A likely explanation for these ions is photoionization by solar ultraviolet radiation of neutral O2 molecules associated with a tenuous ring atmosphere. INMS neutral measurements made during the ring encounter are dominated by a background signal. 相似文献
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