Bacterial community analysis of purulent material from liver abscesses of crossbred cattle and Holstein steers fed finishing diets with or without tylosin

Liver abscesses in feedlot cattle are polymicrobial infections. Culture-based studies have identified Fusobacterium necrophorum as the primary causative agent, but a number of other bacterial species are frequently isolated. The incidence of liver abscesses is highly variable and is affected by a number of factors, including cattle type. Holstein steers raised for beef production have a higher incidence than crossbred feedlot cattle. Tylosin is the commonly used antimicrobial feed additive to reduce the incidence of liver abscesses. The objective of this study was to utilize 16S ribosomal RNA amplicon sequence analyses to analyze the bacterial community composition of purulent material of liver abscesses of crossbred cattle (n = 24) and Holstein steers (n = 24), each fed finishing diet with or without tylosin. DNA was extracted and the V3 and V4 regions of the 16S rRNA gene were amplified, sequenced, and analyzed. The minimum, mean, and maximum sequence reads per sample were 996, 177,070, and 877,770, respectively, across all the liver abscess samples. Sequence analyses identified 5 phyla, 14 families, 98 genera, and 102 amplicon sequence variants (ASV) in the 4 treatment groups. The dominant phyla identified were Fusobacteria (52% of total reads) and Proteobacteria (33%). Of the top 25 genera identified, 17 genera were Gram negative and 8 were Gram positive. The top 3 genera, which accounted for 75% of the total reads, in the order of abundance, were Fusobacterium, Pseudomonas, and Bacteroides. The relative abundance, expressed as percent of total reads, of phyla, family, and genera did not differ (P > 0.05) between the 4 treatment groups. Generic richness and evenness, determined by Shannon–Weiner and Simpson’s diversity indices, respectively, did not differ between the groups. The UniFrac distance matrices data revealed no clustering of the ASV indicating variance between the samples within each treatment group. Co-occurrence network analysis at the genus level indicated a strong association of Fusobacterium with 15 other genera, and not all of them have been previously isolated from liver abscesses. In conclusion, the culture-independent method identified the bacterial composition of liver abscesses as predominantly Gram negative and Fusobacterium as the dominant genus, followed by Pseudomonas. The bacterial community composition did not differ between crossbred and Holstein steers fed finishing diets with or without tylosin.     

Cavity Formation in Thin Films of Viscous Liquids

High-speed photography was utilized to observe the dynamic growth behavior of a cavity in a viscous liquid. From these observations it has been shown that a relatively elementary analytical approach will reproduce the general cavity growth characteristics.     

Use of positive contrast rhinography and intranasal sinography for diagnosis of a nasofacial sinus tract in a dog

Twelve days after a dog fight, a 2-year-old sexually intact female Bulldog was evaluated because of subcutaneous emphysema of increasing severity throughout the dogs body. Thoracic radiography revealed severe pneumomediastinum from which free air had extended into the retroperitoneal space, resulting in pneumoperitoneum. Tracheoscopic examination did not reveal a discontinuity of the trachea, pharynx, or larynx. A breach between the nasal cavity and subcutaneous tissues of the nasal region was suspected. Further diagnostic investigations included positive contrast rhinography and intranasal sinography. Via an angiographic catheter inserted into the left naris, positive contrast intranasal sinography revealed a sinus tract extending between the left nasal cavity and the subcutaneous tissue of the dorsal aspect of the nasal planum. Resolution of subcutaneous emphysema, pneumomediastinum, and pneumoretroperitoneum began 1 day after surgical closure of the intranasal opening of the sinus tract. To the authors' knowledge, this radiographic technique has not been reported.     

Monitoring distances travelled by horses using GPS tracking collars

Objective The aims of this work were to (1) develop a low-cost equine movement tracking collar based on readily available components, (2) conduct preliminary studies assessing the effects of both paddock size and internal fence design on the movements of domestic horses, with and without foals at foot, and (3) describe distances moved by mares and their foals. Additional monitoring of free-ranging feral horses was conducted to allow preliminary comparisons with the movement of confined domestic horses. Procedures A lightweight global positioning system (GPS) data logger modified from a personal/vehicle tracker and mounted on a collar was used to monitor the movement of domestic horses in a range of paddock sizes and internal fence designs for 6.5-day periods. Results In the paddocks used (0.8–16 ha), groups of domestic horses exhibited a logarithmic response in mean daily distance travelled as a function of increasing paddock size, tending asymptotically towards approximately 7.5 km/day. The distance moved by newborn foals was similar to their dams, with total distance travelled also dependent on paddock size. Without altering available paddock area, paddock design, with the exception of a spiral design, did not significantly affect mean daily distance travelled. Feral horses (17.9 km/day) travelled substantially greater mean daily distances than domestic horses (7.2 km/day in 16-ha paddock), even when allowing for larger paddock size. Conclusions Horses kept in stables or small yards and paddocks are quite sedentary in comparison with their feral relatives. For a given paddock area, most designs did not significantly affect mean daily distance travelled.     

Paleomagnetic study of antarctic deep-sea cores

The magnetic inclinations and inten sities of about 650 samples from seven deepsea cores taken in the Antarctic were measured on a spinner magnetometer. This series of measurements provided a magnetic stratigraphy, based on zones of normally or reversally polar ized specimens for each core, which was then correlated with the magnetic stra tigraphy of Cox et al. (1). One core (V16-134) gave a continuous record of the paleomagnetic field back to about 3.5 million years. When selected samples were subject ed to alternatingfield demagnetization, most were found to have an unstable component that was removed by fields of 150 oersteds; all samples from two cores were partially demagnetized in a field of 150 oersteds. The average inclination in these two cores was then in good agreement with the average inclination of the ambient field for the latitude of the core site. It was also found that the intensities of the samples decreased at the points of reversal; this finding is to be expected if, as has been postulated by the dynamo theory, the intensity of the dipole field decreases to zero and builds again with opposite polarity. We believe that the magnetiza tion of the cores results from the pres ence of detrital magnetite, although other magnetic minerals also may be present. Four faunal zones (, X, , and ) have been recognized in these Antarctic cores on the basis of upward sequential disappearance of Radiolaria. The faunal boundaries and reversals consistently have the same relations to one another, indicating that they are both timedependent phenomena. Using previously determined times of reversal, one may date the following events in the cores: 1) Radiolarian faunal boundaries:-X, 2 million years; X-, 0.7 million years; -, 0.4 to 0.5 million years. These dates are in good agreement with ages previously extrapolated from radio metric dates. 2) Initiation of Antarctic diatom ooze deposition, approximately 2.0 mil-lion years ago. 3) First occurrence of ice- rafted detritus, approximately 2.5 million years ago. One can also calculate rates of sedi mentation, which vary in the cores studied from 1.1 to about 8.0 millimeters per 1000 years. Sedimentation rates for the Indian Ocean cores are higher than for the Bellingshausen Sea cores. The near coincidence of faunal changes and reversals in the cores suggests but does not prove a causal relation. We conclude from this study that paleomagnetic stratigraphy is a unique method for correlating and dating deep sea cores, and that future work with such cores may provide a complete or nearly complete record of the history of the earth's magnetic field beyond 4 million years.