Inactivation of infectious pancreatic necrosis virus for vaccine use

Abstract. Formalin and β-propiolactone (BPL) were compared for efficacy in inactivating infectious pancreatic necrosis virus (IPNV) for vaccine use. Incubation with a 1:200 dilution of formalin at 20°C for four days or longer, or a 1:200 dilution of BPL at 4°C for six days or longer, completely inactivated the virus infectivity. However, whereas treatment with formalin caused only a slight reduction in anti-genicity as titrated by in vitro tests, treatment with BPL destroyed over 50% of the antigenicity of the virus. Virus inactivated with formalin also proved highly effective at inducing high titres of neutralizing antibody in trout.     

In this issue – June 2011

Computer model predictions and field observations of anthelmintic resistance in sheep · Dangers of off‐label use of barium selenate · Elbow luxation in dogs and cats · Prognosis of joint infections in adult horses · Omentalisation for mediastinal abscess in a dog · Adenoviruses in lizards     

Rhizomania disease of sugar beet in England

Rhizomania disease was first detected in the UK in 1987, in a single crop in Suffolk. Affected plants had pale leaves, often upright, narrow and rolled; roots were small, often with constrictions, warty outgrowths, proliferation of fibrous roots, and vascular staining. Disease occurred in strips at right angles to one another, parallel with directions of cultivation, suggesting that the previous beet crop had also been infected. Beet necrotic yellow vein virus was detected by ELISA, by electron microscopy, and by transmission to indicator species. It was sometimes associated with beet soil-borne virus. The affected crop was destroyed with herbicide. No other outbreaks were detected in subsequent surveys of crops in 1987.     

On difficulties with allelopathy methodology

Summary. The main techniques and the common experimental methods used in studying allelopathy are reviewed. Problems associated with both methodology and conclusions drawn based on certain techniques are discussed. The accuracy obtained using these techniques is evaluated and show that some of the techniques could be a misleading tool. Some changes are suggested.     

Possible rôle of allelopathy in the competition between tomato, Senecio vulgaris L. and Chenopodium album L.

Summary. The allelopathic effect of Chenopodium album L. (fat-hen) and Senecio vulgaris L. (groundsel) on tomato ( Lycopersicon esculentum Mill cv. Ailsa Craig) plants was investigated under glasshouse conditions. A new technology was used to separate competitive from allelopathic effects. These experiments suggested that C. album had an effect on tomato through allelopathy. C. album leach-ates significantly reduced shoot fresh and dry weights and the accumulation of N, P, K, Ca and Mg of tomato shoots. No effect was found on the quantities of these elements in tomato roots. Leachate of five plants of C. album per pot was sufficient to reduce tomato growth. Addition of 10 or 20 g kg⁻¹ of C. album dried shoots to the soil mixture significantly decreased the fresh and dry weights of tomato plants. S. vulgaris leachates did not show significant effects on the growth or nutrient accumulation of tomato plants.     

The influence of inoculum concentration, relative humidity, and temperature on infection of greenhouse tomatoes by Botrytis cinerea

Botrytis cinerea causes serious crop losses in greenhouse tomato crops through infection of flowers and stem wounds. Experiments were carried out to determine the effects of inoculum concentration, relative humidity (RH), and temperature at these two infection sites. Infection of permanent flower parts increased as a function of inoculum concentration and both length of exposure to high RH (approximately 100% for 0–36 h) and specified continuous RH (56–100%). A low level of infection was still evident under continuous 56% RH. Interruption of periods of high RH with breaks of low RH did not reduce infection. Infection of stem wounds was less dependent on inoculum concentration or RH. Factorial combinations of inoculum concentration, RH, and temperature produced significant interactions. Higher temperature increased infection of flowers but reduced infection of stem wounds. The main implications for control in commercial crops are as follows. Lowering the aerial spore concentration by maintaining the disease at a low level will reduce flower infection. Lowering RH will reduce but not eliminate flower infection but will have only a small effect on stem infection. Raising the temperature (from 15 to 25°C) will reduce stem infection, and whilst flower infection increases, this is counteracted by increased flower production and a decrease in the proportion of infections reaching the peduncle and stem.     

Vegetation and Environment in Afforested Sand Dunes at Newborough, Anglesey

Newborough Forest was planted with pines, chiefly Corsican pine,during the period 1947–65. In 1986, its soils and vegetationwere surveyed and related to the preplanting condition of thedunes, inferred from air photographs. The youngest dunes contained4 per cent calcium carbonate in the top 30cm; the oldest wereacid, with no free calcium carbonate. There was little acidificationof the soil and no extra leaching of calcium carbonate due tothe pines. Vegetation under trees varied according to the ageand pH of the dunes. Young dunes were almost bare except formoss; older dunes had been colonized by ferns and brambles.All plant species of the original open dune system had apparentlysurvived in unplanted slacks or on road verges; some had increased.Many new species had invaded, including woodland mosses, ferns,orchids and two species of Pyrola.