Recovery of brodifacoum in vomitus following induction of emesis in dogs that had ingested rodenticide bait

AIM: To assess the benefit of inducing emesis in dogs that have ingested rodenticide bait containing brodifacoum (BDF), by determining the amount of BDF in bait recovered from the vomitus relative to the estimated amount consumed.

METHODS: Between 2014 and 2015 samples of vomitus from seven dogs that ingested rodenticide baits containing BDF were submitted by veterinarians in New Zealand. All seven dogs had been given apomorphine by the veterinarian and vomited within 1 hour of ingesting the bait. Some or all of the bait particles were retrieved from each sample and were analysed for concentrations of BDF using HPLC. Based on estimations of the mass of bait consumed, the concentration of BDF stated on the product label, and the estimated mass of bait in the vomitus of each dog, the amount of BDF in the vomited bait was calculated as a percentage of the amount ingested.

RESULTS: For five dogs an estimation of the mass of bait ingested was provided by the submitting veterinarian. For these dogs the estimated percentage of BDF in the bait retrieved from the vomitus was between 10–77%. All dogs were well after discharge but only one dog returned for further testing. This dog had a normal prothrombin time 3 days after ingestion.

CONCLUSIONS AND CLINICAL RELEVANCE: The induction of emesis within 1 hour of ingestion can be a useful tool in reducing the exposure of dogs to a toxic dose of BDF. The BDF was not fully absorbed within 1 hour of ingestion suggesting that the early induction of emesis can remove bait containing BDF before it can be fully absorbed.     

Multi-phase cross-correlation method for motion estimation of fertiliser granules during centrifugal spreading

Excessive fertiliser use has been a main contributor to the increasing environmental imbalance observed in the past 20 years. Better accuracy in spreading would limit excess fertiliser loss into the environment. Increased accuracy begins by understanding the fertiliser spreading process from the vane to the soil. Our work concentrates on the use of centrifugal spreaders, as these are most commonly used in Europe. Progress in imaging devices and image processing has resulted in the availability of new technologies to use when describing the behaviour of fertiliser granules during ejection from centrifugal spreaders. Fertiliser deposition on the soil can be predicted using a ballistic flight model, but this requires determination of the velocities and the directions of the granules when they leave the spinning disc. This paper presents improvements to the high speed imaging system that we had previously developed, i.e. enhancements to the illumination and the image processing. The illumination of the previous system, which used many separate flashes, did not give consistent illumination. We have improved it by using a stroboscope with power-LEDs, located at 1 m height around the digital camera and controlled by a Field-programmable gate array (FPGA) card. The image processing has been improved by development of a multi-phase method based on a cross-correlation algorithm. We have compared the cross-correlation method to the Markov Random Fields (MRF) method previously implemented. These tests, based on multi-exposure images, revealed that cross-correlation method gives more accurate results than the MRF technique, with guaranteed sub-pixel accuracy. Knowing that an error of one pixel can lead to a prediction error between 200 and 500 mm on the ground, the latter method gives an accuracy range between 0.1 and 0.4 pixels, whereas the MRFs technique is limited to 3 and 9 pixels for the vertical and horizontal components of the velocities, respectively. The sub-pixel accuracy of the new method was proven by applying it on simulated images with known displacements between the grains. By using a realistic spreading model, the simulated images are similar to those obtained with a high speed imaging system. This sub-pixel accuracy now makes it possible to decrease the resolution of the camera to that of a classical high-speed camera. These improvements have created an affordable and durable system appropriate for installation on a spreader. Farmers could use this system to both calibrate the spreader and verify the fertiliser distribution on the ground.     

Identification of bluetongue virus serotype 2 (Corsican strain) by reverse-transcriptase PCR reaction analysis of segment 2 of the genome

In October 2000, bluetongue virus was detected on the French island of Corsica. The disease was also reported in Sardinia, Calabria, Sicily and on the Spanish islands of Majorca and Minorca. This paper describes the use of molecular techniques for a rapid identification and serotype determination of serotype 2 of the virus. The nucleotide sequences of segments 2 and 7 of the genome of the Corsican strain were determined and its phylogenetic relationships are described.     

The interaction among age,thermal acclimation and growth rate in determining muscle metabolic capacities and tissue masses in the threespine stickleback,Gasterosteus aculeatus

Thermal acclimation may directly modify muscle metabolic capacities, or may modify them indirectly via effects upon physiological processes such as growth, reproduction or senescence. To evaluate these interacting effects, we examined the influence of thermal acclimation and acclimatization upon muscle metabolic capacities and tissue masses in 1 + stickleback, Gasterosteus aculeatus, in which confounding interactions between temperature and senescense should be absent. Furthermore, we examined the influence of thermal acclimation upon individual growth rate, muscle enzyme levels and tissue masses in 2 + stickleback sampled at the beginning of their final reproductive season. For 1 + stickleback, cold acclimation more than doubles mitochondrial enzyme levels in the axial muscle. Thermal acclimation did not change the condition of 1 + stickleback at feeding levels which could not maintain the condition of 2+ stickleback. Compensatory metabolic responses to temperature were not apparent in field acclimatized 1 + stickleback. The growth rate of 2 + stickleback was markedly affected by temperature: warm-acclimated fish generally lost mass even at very high levels of feeding (up to 78 enchytraid worms per day) while cold-acclimated fish gained mass. This suggests that warm temperatures accelerate the senescence of 2 + stickleback. Generally, muscle enzyme activities increased with growth rate. In axial muscle, the relationships between CS activity and growth rate differed with acclimation temperature. Independent of the influence of growth rate, CS activities were consistently higher in cold- than warm-acclimated 2 + stickleback, suggesting compensatory increases of CS activity with cold acclimation. Corresponding author; This paper is dedicated to the memory of Gerry J. FitzGerald who passed away on March 14, 1994.     

The conservation status of Sabellaria alveolata (L.) (Polychaeta: Sabellariidae) reefs in the Bay of Mont‐Saint‐Michel

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Variation for plant characters and for performance of softwood cuttings of Rosa canina ‘Inermis’ seedlings

Summary Thirty, one-season-old Rosa canina Inermis seedlings (8–12 mm in diameter) were grown in the greenhouse. After 5 months, single-node softwood cuttings were made of each plant. Analysis of variance demonstrated marked differences between the internode length, number of leaflets per leaf and the leaf area of the original seedling, and between the time of axillary bud-break, axillary sprout length and weight of adventitious roots of their 23-day-old cuttings. Clonal root weight of cuttings was highly significantly correlated with: number of leaflets per leaf (r=–0.65), leaf area (r=+0.65), and days to axillary bud-break (r=+0.62). The origin of heterogeneity among Inermis seedling rootstocks, its probable effect on the flower production of cut roses and its possible use in the selection for clonal rootstocks are discussed.     

Identification on commercialized products of AFLP markers able to discriminate slow- from fast-growing chicken strains

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