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Tight junctions (TJs) in inter-Sertoli junctional areas and epididymal epithelia build up the blood–testis barrier (BTB) and the blood–epididymal barrier (BEB), respectively. In this study, the expression of occludin, an integral member of the TJs, was examined in testis and different regions of epididymis of Lepus sinensis coreanus , an Korean wild rabbit species. In testis, intense occludin immunoreactivity was found in the basally located inter-Sertoli junctional area together with diffused immunoreactivity of occludin in the cytoplasm of Sertoli cells. It can be suggested that occludin is one of the robust elements of BTB in seminiferous tubules of rabbit testis. In proximal and distal caput epididymis, occludin immunoreactivity was found in the lateral as well as apical contacts of epithelial cells. In corpus epididymis, intense occludin immunoreactivity was found in the basolateral as well as apical contacts of epithelial cells together with cytoplasmic signal. In cauda epididymis, occludin immunoreactivity in luminal epithelia was relatively strong but largely found in the cytoplasm. This suggests that intriguing regulatory mechanisms differentially recruit occludin to the TJ in the different regions of epididymal epithelia. The differences in the subcellular localization as well as expression levels of occludin among the epididymal segments may reflect differential paracellular permeability of epithelia along the epididymal tubules and be correlated with sperm maturation in rabbit. In Western blot, a major form of occludin was MW 62 kDa together with small fragments of MW 34–39 kDa in testis and epididymis, suggesting the peptide cleavage of occludin. This is the first report on the molecular nature of TJs in a wild rabbit testis and epididymis. 相似文献
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Teratogenic polydactyly in a halfbred foal 总被引:1,自引:0,他引:1
M A Dore 《The Veterinary record》1989,125(14):375-376
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The possibility of clonal propagation of banana through tissue culture was explored. Shoot tips isolated from the rhizomes were found to be suitable material for plantlet production in vitro. Excised shoot tips with the youngest leaves produced only one plantlet. However, shoot tips with several older sheathing leaf bases enclosing the axilary buds regenerated multiple plantlets. Individual shootlets, when separated and sub-cultured, produced a new crop of multiple shoots. The plantlets obtained from both types of explants have been successfully transplanted to soil and grown to maturity. 相似文献
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The introduction of a living cover crop during a cash crop growth cycle (relay intercropping) and its maintenance after the cash crop harvest may help to preserve biodiversity, increase soil organic matter content and carbon sequestration and provide other ecosystem services, such as natural pest regulation or nutrient recycling, by increasing useful biotic interactions within the agroecosystem. We studied the impact of various approaches to manage a red fescue cover crop in a winter wheat crop in terms of light, water and nitrogen competition, using the STICS crop model adapted for intercropping. The STICS model for wheat/fescue intercropping was first evaluated on two years of experimental data obtained in the field. It gave satisfactory statistical results for the prediction of dry matter, leaf area index (LAI) and nitrogen accumulation in the two species, and for nitrogen and water dynamics in the soil. By simulating unmeasured variables, such as transpiration, the model improves our understanding of the performance of the intercrop in the field. For example, we showed that the intercropping system was more efficient that wheat grown as a sole crop, in terms of nitrogen accumulation and decreasing soil nitrogen levels before the leaching period. However, it also resulted in lower wheat yields. We then used the STICS model to compare four intercropping management scenarios differing in terms of the date of red fescue emergence, over 35 climatic years. We found that, in most climatic scenarios, the emergence of the fescue crop during the late tillering phase of the wheat crop gave the best compromise between wheat yield overall nitrogen accumulation and radiation interception. 相似文献