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1.
Yuanfa He Shuyan Chi Beiping Tan Xiaohui Dong Qihui Yang Hongyu Liu Shuang Zhang 《Journal of the World Aquaculture Society》2021,52(1):171-183
A 4‐week feeding trial was conducted using five groups of hybrid grouper (61.15 ± 0.15 g) to explore the potential effects of three methionine (Met) sources. Five isonitrogenous and isolipidic diets were formulated that included a fishmeal (FM) diet; nonsupplemented (NS) diet; or NS diets with the addition of the L‐methionine (L‐Met), DL‐methionine (DL‐Met), or coated Met (Co‐Met) to obtain the same Met level as the FM diet. Fish were randomly distributed into sea cages (30 fish per cage). Weight gain and specific growth rate in the DL‐Met group and FM group were significantly higher than those in all other groups (p < .05). In the proximal and distal intestines, of the 20 gene and time combinations (10 per gene), there were 14 combinations (70%) in which there were no significant differences in gene expression levels between the FM and DL‐Met groups (p > .05). The main reason for the same growth effect between the DL‐Met and FM groups may be attributed to the synchronized absorption at most time points after feeding, which had similar expression patterns of B0AT1 and ASCT2 of the proximal and distal intestines between two groups. 相似文献
2.
为了解中国不同麦区小麦种质资源籽粒脂肪氧化酶(lipoxygenase,LOX)活性相关基因TaLox-B1的差异和分布,利用小麦4B染色体上的功能标记LOX16和LOX18对7个麦区的436份种质资源进行分子检测。结果表明:在供试材料中共检测到3种TaLox-B1基因等位变异类型,分别为TaLox-B1a(与高LOX活性相关)、TaLox-B1b(与低LOX活性相关)和杂合型,其频率分别为19.0%、70.4%和10.6%。小麦LOX活性基因不同变异类型在各生态区的分布存在明显差异:基因型TaLox-B1a在黄淮冬麦区、北部冬麦区和长江中下游冬麦区分布较多,其比例分别为21.1%、19.8%和17.6%;基因型TaLox-B1b在西南冬麦区和长江中下游冬麦区分布较多,比例分别为87.9%、72.5%;杂合型仅存在于北部冬麦区、黄淮冬麦区与长江中下游冬麦区,比例分别为14.2%、12.4%和9.8%。利用标记LOX16和LOX18对53个自选高代品系进行分子检测,发现自选品系仅有TaLox-B1b与杂合型两种基因型,其中基因型TaLox-B1ab有32个,比例为60.4%。采用分子标记辅助选择,有利于快速鉴定小麦籽粒LOX活性,加速LOX的遗传改良和新品种选育。 相似文献
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云南怒江傈僳族自治州古树名木资源现状调查 总被引:1,自引:0,他引:1
对怒江傈僳族自治州人为活动区域古树名木资源现状进行调查。结果表明:共记录到古树名木496株,涉及36科54属76种,其中有8种国家重点保护野生植物,分别为澜沧黄杉、秃杉、南方红豆杉、云南榧树、千果榄仁、红椿、光叶珙桐和香果树;怒江州古树名木主要分布在乡村街道及远郊野外,占总数的98.8%,63.6%的古树生长在海拔1500~1999 m;古树名木的生长环境及长势总体较好,但保护力度相对薄弱,应采取相关措施加强保护和管理。 相似文献
5.
Wang Zhun Zhang Hengheng Dong Qiang Gui Huiping Wang Xiangru Pang Nianchang Li Yongnian Niu Jing Jin Dingsha Wang Sujie Zhang Xiling Song Meizhen 《棉花学报》2021,32(6):538-551
[Objective] The aim of this study is to evaluate nitrogen efficient cotton germplasms and improve nitrogen use efficiency. [Method] Eighty cotton germplasms were selected and evaluated in the hydroponic experiment under low (0.25 mmol·L-1) and high (5 mmol·L-1) nitrogen concentration. Different traits for screening were identified and nitrogen use efficiency types were classified. Field experiments were also performed for comparison and confirmation of the identified germplasms. [Result] The results showed that there were significant differences in the total plant dry matter, shoot nitrogen accumulation and nitrogen absorption efficiency in cotton germplasms at the two nitrogen levels. Based on coefficient of variation, principal component analysis and correlation, six traits including total plant dry matter, shoot dry matter, root dry matter, total nitrogen accumulation, shoot nitrogen accumulation and nitrogen absorption efficiency were used as screening indicators. According to the Heatmap clustering analysis and the nitrogen efficiency comprehensive index, two germplasms (Lu05R59 and CCRI 69) were identified as low nitrogen tolerant and nitrogen efficient, and two germplasms (Coker 201 and Xinluzhong 30) as low nitrogen sensitive and nitrogen inefficient. The results of field experiment were consistent with the results of the hydroponic culture at the seedling stage. [Conclusion] It was finally determined that Lu05R59 and CCRI 69 were the low nitrogen tolerant and nitrogen efficient germplasms, and Coker 201 and Xinluzhong 30 were low nitrogen sensitive and nitrogen inefficient germplasms. The results of these studies provide the possibility for screening and rapid identification of nitrogen use efficiency in cotton at the seedling stage, and provide the ideal materials and theoretical basis for further study of cotton nitrogen efficient. 相似文献
6.
为探究同期排卵处理母牛体温和活动量变化规律及不同同期排卵技术处理效果,指导同期排卵技术优化。本研究自动监测了18头20月龄左右同期排卵(GnRH-PG-GnRH)处理荷斯坦母牛和17头产后40~60 d预同期排卵(PG-PG-GnRH-PG-GnRH)处理荷斯坦母牛的体温和活动量,应用自动检测系统进行母牛发情监测。结果发现,同期排卵处理母牛发情时阴道温度平均升高(0.43±0.20)℃,持续(12.37±2.73)h;活动量平均升高(18.28±18.61)倍,持续(11.00±1.68)h;排卵时阴道温度平均下降(0.20±0.10)℃,持续(11.00±1.68)h。自动化发情监测显示,同期排卵处理母牛7头发情并排卵;预同期排卵母牛GnRH处理前全部发情排卵。两种同期排卵处理,虽可改变母牛性周期进程,促进母牛性周期同步化,但均难以使母牛性周期完全同步。因此,将同期排卵-定时输精和发情鉴定技术科学结合才能取得更好的繁殖效果。 相似文献
7.
The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy. 相似文献
8.
水霉属菌(Saprolegnia)分离培养技术研究 总被引:1,自引:0,他引:1
对水霉(Saprolegnia)的分离培养技术进行了探索,筛选出大麻籽、油菜籽、红花籽、苍蝇、蚜虫、小麦粒、玉米片、茶叶、葵花仁9种材料作为诱发水霉菌的诱饵。其中油菜籽能防止腐霉菌(Pythium)污染,是初步纯化菌种、排除杂菌的适宜饵料。同时发现控制温度能使水霉在最短时间内分别产生无性或有性繁殖。在水培条件下,25℃左右适合菌丝及无性器官的发育,有性器官却不能产生,而只有在15~17℃条件下才能使水霉很快进入有性生殖阶段;在加入200mg/L链霉素的CMA培养基上促发有性阶段则需要在25℃左右的温度下进行,方能缩短培养时间。 相似文献
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