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1.
Can Microfiltered Seminal Plasma Preserve the Morphofunctional Characteristics of Porcine Spermatozoa in the Absence of Antibiotics? A Preliminary Study 下载免费PDF全文
F Barone D Ventrella A Zannoni M Forni ML Bacci 《Reproduction in domestic animals》2016,51(4):604-610
Artificial insemination is extensively performed in pig farms in Europe, the United States and Canada. Antibiotics are typically added to the inseminating dose to limit bacterial growth during liquid phase storage at 16°C, as bacterial contamination is unavoidable. The World Organization for Animal Health (OIE) and the Food and Agriculture Organization (FAO) take action to control and reduce antibiotic use in animals as more bacteria are becoming resistant to antimicrobials. To avoid the use of antibiotics, we prepared inseminating doses using microfiltered seminal plasma (SP). Microfiltration is a common technology used to reduce bacterial contamination but may retain seminal substances, influencing sperm quality during storage. Therefore, the aim of this study was to evaluate the morphofunctional parameters of spermatozoa during storage at 16°C in doses prepared with or without microfiltered SP, with or without the addition of antibiotics, in a Latin square design. Artificial insemination doses with microfiltered SP and without antibiotic addition preserved spermatozoa viability, mitochondrial membrane potential, acrosome integrity and objective motility, with absolute values equal or even better than those observed in conventional doses. In conclusion, although the results could be considered preliminary due to the small sample size, this study suggests that microfiltration of SP can be a simple method, feasible on farms, to replace antibiotic use in extended doses stored in the liquid phase at 16°C for up to 7 days. 相似文献
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Elke Rudloff DVM Dennis T. Crowe DVM Jr. Rebecca Kirby DVM Bobbie Mammato DVM MPH 《Journal of Veterinary Emergency and Critical Care》1996,6(2):103-107
Successful resuscitation of a trauma-induced, suspected tension pneumomediastinum in a dog is presented. The cause was a caudal cervical penetration wound which acted as a one-way valve allowing air to enter, but not exit, the cranial mediastium. Early in the course of hospitalization, respiratory and cardiovascular collapse occurred; an emergent parasternotomy allowed decompression of the mediastinum, and the cradiovascular system immediately responded. The dog made a complete recovery following surgical exploration and removal of contaminated mediastinal tissue. Nine days of intensive care included 24-hour monitoring, oxygen therapy, intravenous crystalloids and colloids, continuous pleural suctioning, nutritional support, antibiotics, and pain control. 相似文献
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REASONS FOR PERFORMING STUDY: Proximal suspensory desmitis (PSD) is diagnosed with increasing frequency in horses and radial pressure wave therapy (RPWT) is a widely used therapy for painful orthopaedic conditions in man and dogs. There are, however, few published data as to the outcome of its use in PSD. OBJECTIVE: To evaluate the use of RPWT in the treatment of chronic or recurrent PSD in the horse, an injury which carries a poor prognosis for return to athletic function with conservative management alone. HYPOTHESIS: RPWT and controlled exercise improves the prognosis of chronic or recurrent PSD in the horse when compared to previously published results of controlled exercise alone. METHODS: The use of RPWT in the management of chronic or recurrent proximal suspensory desmitis (PSD) was evaluated in 65 horses. Diagnosis was based on response to local analgesia, ultrasonography and radiography. Horses were classified according to severity of ultrasonographic lesions, whether fore- or hindlimbs were affected, and duration of lameness prior to diagnosis. Horses were treated 3 times at 2-week intervals and followed a controlled exercise programme; they were reassessed clinically and ultrasonographically 10-12 weeks after diagnosis, when further exercise recommendations were made dependent upon the animal's progress. RESULTS: Forty-one percent of horses with hindlimb lameness and 53% with forelimb lameness were nonlame and returned to full work 6 months after diagnosis. The prognosis was significantly affected by the ultrasonographic grade at the time of diagnosis and by ultrasonographic evidence of resolution of the lesion in hindlimb cases. CONCLUSIONS: These findings, when compared to previously published results of treatment using controlled exercise alone, suggest that RPWT improves the prognosis for PSD in the hindlimb. POTENTIAL RELEVANCE: RPWT is a useful treatment modality for chronic or recurrent PSD when combined with controlled exercise. Further studies are required on the effect of RPWT employing histology and biomechanics in order to fully evaluate its use on equine tissues. 相似文献
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The objective was to assess the effect of banding or burdizzo castration performed on farms on plasma testosterone, acute-phase proteins, scrotal circumferences, growth, and well-being of bulls. 243 Continental bulls (12 months; 399.2 ± 5.72 kg) from three different farms were allocated at random, after stratification on weight within breed type, to one of three treatment groups: banding castration (BAND; n = 80), burdizzo castration (BURD; n = 83), or controls (CON; n = 80). The castration methods were conducted under local anaesthesia, and tetanus toxoid vaccine and antibiotic were also injected at castration. BAND and BURD castrates had lower (P < 0.001) plasma testosterone concentration than control bulls, with no difference between BAND and BURD castrates on 28 d post-castration. From days 0 to 14 post-castration, BAND (P = 0.0002) and BURD (P < 0.0001) castrates had lower average daily gain (ADG) than CON bulls, no difference (P = 0.46) was found between BAND and BURD castrates. From days 15 to 28, BAND castrates had lower ADG compared with BURD castrates (P = 0.03) and CON bulls (P = 0.01), while no difference (P = 0.76) was found between BURD and CON. From days 29 to 56, BAND (P = 0.01) and BURD (P = 0.002) castrates had lower ADG than CON bulls, no difference (P = 0.55) was found between BAND and BURD. From days 57 to 84, the ADG of BAND castrates was not different compared with BURD castrates (P = 0.12) and CON bulls (P = 0.38), while BURD had lower (P = 0.02) ADG compared with CON. The integrated ADG from day 0 to 112 of BAND (P = 0.0001) and BURD (P = 0.02) groups were lower compared with CON, while there was no difference (P = 0.09) between BAND and BURD castrates. On d 14 post-castration, BAND castrates had lower scrotal temperature than BURD (P < 0.0001) and CON (P < 0.0001), and BURD castrates had greater (P < 0.006) scrotal temperature than CON; BAND castrates had lower scrotal latitudinal and longitudinal circumferences than BURD castrates (P < 0.001) and CON bulls (P < 0.001), and BURD castrates had greater (P < 0.001) scrotal latitudinal and longitudinal circumferences than CON bulls. BAND (P < 0.0001) and BURD (P = 0.01) castrates had greater glucose concentration than CON bulls, and BAND castrates had greater (P = 0.04) glucose concentration than BURD. In conclusion, BAND or BURD castration significantly reduced plasma testosterone concentration; reduced average daily weight gain mainly during the first 2 weeks, which was not compensated during the subsequent 16 weeks; increased withdrawal of stored energy and increased plasma protein concentration. BURD showed an advantage over BAND in growth during days 15 to 28 following castration. 相似文献
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TS Gaggini J Perin LS Arend ML Bernardi I Wentz FP Bortolozzo 《Reproduction in domestic animals》2013,48(3):390-395
This study investigated the effect of altrenogest treatment on the farrowing development of sows, and birth weight (BW) and piglet survival until the third day of life. Three control groups were used: (i) sows that farrowed spontaneously before 114 day of gestation (CONT <114); (ii) sows that spontaneously farrowed at ≥114 day of gestation (CONT ≥114); (iii) sows that farrowed at ≥114 day with cloprostenol treatment (CONTCLOPR). Other sows were treated with altrenogest (Regumate®) for 3 days (days 111, 112 and 113 of gestation): one group gave birth spontaneously (ALT) and the other group received altrenogest until day 113 and cloprostenol on day 114 (ALTCLOPR). There were no differences (p > 0.05) in farrowing duration, BW, coefficient of variation (CV) of BW, stillborn piglets, mummified foetuses, percentage of light piglets and survival until Day 3 between sows with and without cloprostenol treatment, in both control (CONT ≥114 vs CONTCLOPR) and altrenogest‐treated sows (ALT vs ALTCLOPR). Further comparisons were performed taking into account three groups: sows with early delivery (CONT <114 – farrowing before 114 days of gestation; n = 56), sows with longer gestation (CONT ≥114 – with and without cloprostenol treatment sows; n = 103) and ALT sows (with and without cloprostenol treatment; n = 105). Gestation length of CONT ≥114 and ALT sows was similar (p > 0.05), but higher than in CONT <114 sows. There were no differences (p > 0.05) between groups in farrowing duration, CV of BW, and percentages of stillborn piglets and mummified foetuses. Sows of CONT <114 group had a larger litter size and a lower BW than sows of the other two groups (p < 0.05). Sows of CONT <114 group had a higher percentage of lighter piglets and a lower piglet survival rate (p < 0.05) than ALT sows. In conclusion, altrenogest treatment proved to be an efficient method to avoid early parturition in 3–5 parity sows resulting in heavier piglets at birth. 相似文献