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1.
本试验是对测定饲料中钙含量的3种方法,即双波长吸光光度法、MSA—DBM显色法和高锰酸钾法的比较试验。综合本实验结果,建议饲料生产中采用MSA—DBM显色法,可以简便快速的得到可靠结果。 相似文献
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V Danielová D Málková J Minár B Rehse-Küpper Z Hájková J Halgos L Jedlicka 《Folia parasitologica》1978,25(2):187-190
In the years 1973 and 1975 mosquitoes and some other Diptera (Tabanidae, Simuliidae, Hippoboscidae) were tested for virus. 13,924 mosquitoes, 75 horseflies and 60 blackflies were processed in 1973. Five strains of Tahyna virus were isolated from mosquito species Aedes vexans. 3,378 mosquitoes and 12 sheep keds were tested for virus in 1975. Twelve strains of Calovo virus were isolated from Anopheles maculipennis and one strain of Tahyna virus was obtained from Aedes vexans mosquitoes. 相似文献
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AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer. 相似文献
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CAI Yun-feng MIN Jun FANG Tian-ling CHU Zhong-hua DENG Xiao-geng HE Jing-song CHEN Ji-sheng 《园艺学报》2003,19(8):1029-1033
AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy. 相似文献
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奶牛“两病”是指奶牛的结核病和布鲁氏菌病,是世界动物卫生组织(OIE)规定强制报告的两种疫病,二病都属人畜共患病。人的牛型结核感染主要是通过饮用生奶引起,尤其是儿童常因喝生牛奶被感染而患肠结核杆菌病;饮用生奶或制品也易感染布鲁氏菌病。奶牛“两病”严重威胁着人民的身体健 相似文献
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红豆草壳二孢叶斑黑茎病(Ascochyta onobrychidis)是红豆草(Onobrychis viciaefolia)最重要的病害之一,在分离此病菌的同时得到一种对此病菌具有较强抑制作用的微生物,为评价此拮抗菌的开发利用前景,本研究采用形态学、分子生物学和生物化学方法鉴定了其分类地位,并测定了其对红豆草壳二孢的抑菌效果。通过构建系统发育树并结合其形态特征和生理生化特性,将该拮抗菌鉴定为微白黄链霉菌(Streptomyces albidoflavu),在高氏一号培养基上菌落为黄白色,可产生黄白色水溶性色素,孢子丝钩状,顶端螺旋形;在平板对峙试验中该菌的菌落对红豆草壳二孢菌落的相对抑制率达74.33%,同时该菌发酵液对红豆草壳二孢菌丝生长和孢子萌发的抑制率分别为80.95%和93.87%。该菌具有生物防治红豆草壳二孢的开发和利用价值。 相似文献
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