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1.
AIMS: To determine if abdominal insufflation with medical air will improve oxygenation and ventilation parameters when compared to insufflation with CO2 in xylazine-sedated sheep undergoing laparoscopic artificial insemination (AI).METHODS: Forty-seven sheep underwent oestrus synchronisation and were fasted for 24 hours prior to laparoscopic AI. Each animal was randomised to receive either CO2 or medical air for abdominal insufflation. An auricular arterial catheter was placed and utilised for serial blood sampling. Respiratory rates (RR) and arterial blood samples were collected at baseline, after xylazine (0.1?mg/kg I/V) sedation, 2 minutes after Trendelenburg positioning, 5 minutes after abdominal insufflation, and 10 minutes after being returned to a standing position. Blood samples were collected in heparinised syringes, stored on ice, and analysed for arterial pH, partial pressure of arterial O2 (PaO2), and CO2 (PaCO2). The number of ewes conceiving to AI was also determined.RESULTS: Repeated measures ANOVA demonstrated temporal effects on RR, PaO2, PaCO2 and arterial pH during the laparoscopic AI procedure (p<0.001), but no difference between insufflation groups (p>0.01). No sheep experienced hypercapnia (PaCO2>50?mmHg) or acidaemia (pH<7.35). Hypoxaemia (PaO2<70?mmHg) was diagnosed during the procedure in 14/22 (64%) ewes in the CO2 group compared with 8/23 (35%) ewes in the medical air group (p=0.053). Overall, 15/20 (75%) ewes in the CO2 group conceived to AI compared with 16/22 (72.7%) in the medical air group (p=0.867).CONCLUSIONS AND CLINICAL RELEVANCE: There were no statistical or clinical differences in RR, PaO2, PaCO2, pH, or conception to AI when comparing the effects of CO2 and medical air as abdominal insufflation gases. None of the sheep experienced hypercapnia or acidaemic, yet 42% (19/45) of sheep developed clinical hypoxaemia, with a higher percentage of ewes in the CO2 group developing hypoxaemia than in the medical air group. Based on the overall analysis, medical air could be utilised as a comparable alternative for abdominal insufflation during laparoscopic AI procedures. 相似文献
2.
SR Cho SA Ock JG Yoo B Mohana kumar SY Choe GJ Rho 《Reproduction in domestic animals》2005,40(2):171-176
The present study was designed to examine the effects of cell-cycle synchronization protocols, such as confluent, roscovitine treatment and serum starvation, in bovine foetal fibroblasts on synchronization accuracy at G0/G1, viability, apoptosis, necrosis and ploidy for use as a nuclei donor. The cells in 5-10 passages were randomly allocated into three treated groups. Cells were cultured either in Dulbecco's modified Eagle's medium (DMEM) + 10% foetal bovine serum (FBS) until 90% confluent (group 1, confluent), in DMEM + 10% FBS + 30 microM roscovitine for 12 h (group 2, roscovitine), or in DMEM + 0.5% FBS for 5 days (group 3, serum starvation). Most of the cells (>80%) in all groups were arrested at the G0/G1 stage. Although the rates did not differ, cells in group 1 showed an increased cell population arrested at the G0/G1 phase. Significantly (p < 0.05) higher rates of apoptosis occurred in group 3 than in group 1 and 2 (10% vs 6% and 6%, respectively). No differences in chromosomal abnormality were observed among groups. However, by increasing the number of cell culture passages up to 15, significantly (p < 0.05) higher chromosomal abnormality was observed than in 5 and 10 passages (39% vs 28% and 23%, respectively) in group 1. The results clearly indicated that bovine foetal fibroblasts could be effectively synchronized at G0/G1 stages by all the three different treatments, confluent, roscovitine and serum starvation. However, cells in confluent showed reduced apoptosis and necrosis when they underwent 5-10 passages, exhibiting increased percentage of cells with stable chromosome diversity. Hence, cells in confluent merit further studies before they could be used as nuclear donors. 相似文献
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Equine pituitary pars intermedia dysfunction: current understanding and recommendations from the Australian and New Zealand Equine Endocrine Group
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CJ Secombe SR Bailey MA de Laat KJ Hughes AJ Stewart JM Sonis RHH Tan 《Australian veterinary journal》2018,96(7):233-242
The purpose of this article is to provide a review of the current knowledge and opinions about the epidemiology, clinical findings (including sequelae), diagnosis, treatment and monitoring of equine pituitary pars intermedia dysfunction, particularly in the Australian context. This information and the recommendations provided will assist practitioners in making informed decisions regarding the diagnosis and management of this disorder. 相似文献
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Differential HSP90α expression in fish hepatocytes from polluted estuary during summer 总被引:2,自引:0,他引:2
The molecular chaperone, heat shock protein 90α (HSP90α), plays an important role in protein folding, degradation of denatured
proteins and steroid activation. It is essential for the maintenance of cellular integrity and survival when induced in response
to environmental, physical and chemical stresses. In the present investigation the effect of environmental stress on HSP90α
expression was examined in grey mullet Mugil cephalus living in either a contaminated (Ennore) or uncontaminated (Kovalam) estuary over two seasons: Hepatocytes were isolated
from grey mullet of both estuaries. Oxidative stress was determined along with HSP90α in these fish. Additionally, immunohistochemical
changes were studied to confirm the HSP90α expression. Comparison of the results revealed enhanced hepatocyte oxidative stress
and HSP90α expressions in fish from Ennore to a significant extent than fish from Kovalam. Also, the results showed significant
seasonal variations with maximum expression observed during summer compared to the monsoon season. Overexpression of HSP90α
in hepatocytes exposed to chronic environmental stress by pollutants may confer differential effects on cell survival by protecting
against oxidative stress induced changes. The results also indicate that seasonal variations have significant effect on the
HSP90α expression. 相似文献
8.
Cleome gynandra L., commonly known as cat's whiskers, is an erect, branched, annual herb, belonging to the family Cleomaceae. The species is thought to have originated in tropical Africa and Southeast Asia, and thereafter have spread to other tropical and subtropical countries in the Northern and Southern hemispheres. Cat's whiskers is a highly nutritious vegetable and also possesses numerous medicinal values, yet it is considered as a neglected and underutilized weed species (NUS) in most of the world. In India, C. gynandra is recorded in Assam, Gujarat, Kerala, Maharashtra, Rajasthan, Tamil Nadu, and Uttar Pradesh. There is no earlier report of this species in West Bengal, India. This report is the first record of natural occurrence of C. gynandra in West Bengal, India. The main objective of this report is to provide valid information about the invasion and naturalization of this species in a new geographical area supported with suitable data. Global distribution data of cat's whiskers was retrieved from available web resources and mapped using Quantum GIS software in order to validate the de novo nature of occurrence of this species in this region. 相似文献
9.
F. Bose 《Forstwissenschaftliches Centralblatt》1895,17(1):64-67
Ohne Zusammenfassung 相似文献
10.
Bose 《Forstwissenschaftliches Centralblatt》1896,18(1):35-41
Ohne Zusammenfassung 相似文献