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1.
The effect of stocking density on the growth of mulloway, Argyrosomus japonicus, was tested with 17 g fish stocked at 4.08, 8.16, or 16.32 kg m−3 in 50 l aquaria. Weight checks were carried out every 2 weeks to track performance. Each density treatment was also compared to a nonhandled control group to establish if handling during weight checks influenced the growth of mulloway. Mulloway performed poorly at the lowest density and, under the current experiment conditions, growth did not appear to be negatively affected by regular handling.  相似文献   
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Background

Identification of Staphylococci to species level in veterinary microbiology is important to inform therapeutic intervention and management. We report on the efficacy of three routinely used commercial phenotypic methods for staphylococcal species identification, namely API Staph 32 (bioMérieux), RapID (Remel) and Staph-Zym (Rosco Diagnostica) compared to genotyping as a reference method to identify 52 staphylococcal clinical isolates (23 coagulase positive; 29 coagulase negative) from companion animals in Irish veterinary hospitals.

Results

Genotyping of a 412 bp fragment of the staphylococcal tuf gene and coagulase testing were carried out on all 52 veterinary samples along with 7 reference strains. In addition, genotyping of the staphylococcal rpoB gene, as well as PCR-RFLP of the pta gene, were performed to definitively identify members of the Staphylococcus intermedius group (SIG). The API Staph 32 correctly identified all S. aureus isolates (11/11), 83% (10/12) of the SIG species, and 66% (19/29) of the coagulase negative species. RapID and Staph-Zym correctly identified 61% (14/23) and 0% (0/23) respectively of the coagulase-positives, and 10% (3/29) and 3% (1/29) respectively of the coagulase-negative species.

Conclusions

Commercially available phenotypic species identification tests are inadequate for the correct identification of both coagulase negative and coagulase positive staphylococcal species from companion animals. Genotyping using the tuf gene sequence is superior to phenotyping for identification of staphylococcal species of animal origin. However, use of PCR-RFLP of pta gene or rpoB sequencing is recommended as a confirmatory method for discriminating between SIG isolates.  相似文献   
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Controlling microbial contamination on beef and lamb meat during processing   总被引:2,自引:0,他引:2  
SUMMARY The microbiological quality of carcases, meat and environmental surfaces was evaluated in commercial boning rooms processing beef and lamb. There was considerable variation in the level of microbial contamination on both carcases and meat, with counts ranging from less than 20 to 108/cm2 on carcases and to 2 times 107/cm2 on meat. The level of microbial contamination on meat was influenced by the level of carcase contamination at boning and by the boning process itself. Carcase contamination was the major determinant of microbiological quality, as more than 70% of carcases had microbial counts greater than 103/cm2. Cutting boards were a major source for microbial dissemination during boning, particularly when carcase counts were less than 103/cm2. If carcases were heavily contaminated, the contamination of processing surfaces was irrelevant in determining microbial loads on meat. Where carcase contamination was at low to moderate levels, the contribution of the boning process to the contamination on meat assumed increased significance. Under these conditions, improved sanitation of cutting surfaces in the boning room resulted in a significant reduction in microbial contamination on the surface of meat. These results can form the basis for ensuring that improvements made in carcase management before boning, to improve microbiological quality, will be preserved through attention to cutting board hygiene during boning.  相似文献   
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Wildlife viewing recreation offers conservationists opportunities for education and generating revenue but can also have detrimental ecological impacts. To manage these opportunities and impacts effectively, a better understanding is needed of what people value in wildlife viewing events. We examine the relationship between species rarity and value for wildlife viewing recreation. We undertook visitor counts of birdwatchers attending rare (vagrant) bird sightings and collected home postcodes to assess the distances these individuals travelled to achieve these sightings. We also undertook visitor counts at common bird viewing locations for comparison. We regressed birdwatcher numbers against rarity, site protection status, time the bird had been on site and day of the week when the count took place. We undertook these analyses for rare bird sightings only, using a continuous measure of rarity, and for both rare and common species combined, using a categorical rarity index. Species rarity was the clearest predictor of visitor numbers in both the analyses. When studying rare birds only, we found the functional form of the relationship between rarity and visitor numbers to be inverse and asymptotic. Individuals also travelled further to see rarer species. However, while exceptional numbers of visitors attended exceptionally rare bird sightings, the marginal value of rarity appeared to be relatively low. Despite the opportunity for revenue raising and education provided by rare bird sightings, a comparison of visitor numbers at sightings inside and outside protected areas showed no evidence that managers of protected areas capitalise on these opportunities.  相似文献   
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Based on previously reported studies describing the experimental infection of pregnant goats with B. melitensis strain RWP5, we proposed that the HtrA protease plays an important role in the virulence of B. melitensis in its natural ruminant host. Subsequent studies, however, have shown that RWP5 is actually an htrA cycL double mutant. In order to definitively evaluate the role of the B. melitensis htrA in virulence, we constructed an authentic htrA mutant and examined this strain in pregnant goats. The findings of these studies indicate that the contribution of the htrA gene product to the virulence of B. melitensis in its natural host is not as great as was previously proposed.  相似文献   
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The limited field of view (FOV) associated with single-resolution very-large-scale aerial (VLSA) imagery requires users to balance FOV and resolution needs. This balance varies by the specific questions being asked of the data. Here, we tested a FOV-resolution question by comparing ground cover measured in the field with the use of point-intercept transects with similar data measured from 50-mm-per-pixel (mmpp) VLSA imagery of the same locations. Particular care was given to spatial control of ground and aerial sample points from which observations were made, yet percent cover estimates were very different between methods. An error budget was used to calculate error of location and error of quantification. These results indicated location error (43.5%) played a substantial role with significant quantification error (21.6%) also present. We conclude that 1) although the georectification accuracy achieved in this project was actually quite good, the level of accuracy required to match ground and aerial sample points represents an unrealistic expectation with currently available positioning technologies, 2) 50-mmpp VLSA imagery is not adequate for accurate species identification or cover assessments of plant functional groups, and 3) the balance between resolution and FOV needs is best addressed by using multiple cameras to acquire nested imagery at multiple VLSA resolutions simultaneously. We recommend ground cover be measured from 1-mmpp imagery and that the imagery be nested in lower-resolution, larger FOV images simultaneously acquired.  相似文献   
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