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1.
AIMS: To determine the variability of concentrations of Zn in feed, when used as a supplement to prevent facial eczema, and to determine the variability in concentrations of Zn in serum between cows and herds that are being supplemented with ZnO in feed, using in-shed feeders or on a feed pad.METHODS: Sixteen commercial dairy farms in the Waikato region of New Zealand were enrolled, that were supplementing cows with ZnO in the feed using either an automatic in-shed feeder (ASF) or a feed pad (FP) using a feed-out or mixer wagon. On each farm 10 cows were selected by the farmer, that were assumed to be representative of the age and liveweight of the herd. Four hours after supplement feeding, each cow was weighed and a blood sample collected for measurement of concentrations of Zn in serum. Three samples of feed were collected from each farm for Zn analysis, from the beginning, middle and end of the feed being distributed. Levene’s test for homoscedasticity was used to analyse whether there were differences in variation of individual concentrations of Zn in serum, and in the feed, between the two feeding systems. Multivariable linear regression was used to examine associations between age, feeding method or liveweight and concentrations of Zn in serum, after accounting for the variability between farms.RESULTS: Of the 163 cows sampled, concentrations of Zn in serum were between 20–35?µmol/L in 75/163 (46 (95% CI=38–54)%) cows; were <20?µmol/L in 71/163 (44 (95% CI=36–52)%) cows, and >35?µmol/L in 17/163 (10 (95% CI=6–16)%) cows. The variation in concentrations of Zn in serum in individual cows differed between farms (p<0.001), and the variability was greater for cows fed using a FP than ASF (p<0.001). There was no difference in the variation of concentrations of Zn in feed between the two feeding methods (p=0.54), but concentrations of Zn in serum were associated with the amount of Zn offered in feed (p=0.008).CONCLUSIONS AND CLINICIAL RELEVENCE: There was significant variability between farms in the concentrations of Zn in the serum of cows being supplemented with ZnO in feed. Only 46% of cows sampled had concentrations of Zn between 20–35?µmol/L. Effective management of facial eczema should include monitoring Zn in the feed and in serum to ensure cows are receiving the correct dose they require. 相似文献
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Thirty-two accessions of wild tomato (Lycopersicon spp.) germplasm were evaluated for resistance to the whiteflyBemisia argentifolii Bellows ⇐p; Perring in a greenhouse choice bioassay. Density data were recorded for the adaxial and abaxial leaf surfaces
for (i) all life stages ofB. argentifolii and (ii) types I, IV, V and VI trichomes. Individual plant selections (33 from 22 wild tomato accessions) with high resistance
were subsequently tested in the field to verify the resistance found in the greenhouse screening. Resistance was defined by
the density of all life stages of the whitefly observed on the eight leaflets sampled at nodes 5 and 7. Only type IV trichomes
had a consistent (but low) and significant negative correlation between trichome density and whitefly density for various
life stages. The highest whitefly resistance was observed inLycopersicon pennellii accessions LA 716, LA 1340 and LA 2560. The most resistantL. hirsutum f.typicum accessions were LA 1777 and LA 1353.
http://www.phytoparasitica.org posting Dec. 9, 2002. 相似文献
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AM Batista WA Gomes CCD Carvalho PLJ Monteiro Jr FLM Silva FC Almeida PC Soares GF Carneiro MMP Guerra 《Reproduction in domestic animals》2014,49(3):476-480
The aim of this study was to evaluate the effect of leptin administration during superovulation on in vivo goat embryo production. Ten mature does were superovulated with 133 mg follicle‐stimulating hormone (FSH) i.m. in six descending doses at 12‐h intervals. The goats received 4.8 μg/kg human recombinant leptin s.c. (leptin group, n = 5) or phosphate‐buffered saline (PBS) (control group, n = 5) with the first and second FSH doses. The does were mated and subjected to embryo collection by transcervical technique 6 days later. The total number of cells per embryo and the number of cells with fragmented DNA were assessed in selected blastocysts by combining Hoechst 33342 and terminal dUTP nick‐end labelling (TUNEL) staining. Plasma concentrations of oestradiol (E2) and progesterone (P4) were determined by electrochemiluminescence from the day of FSH treatment, on the day of superovulatory oestrus and on the day before embryo collection. Compared with the control group, the does that received leptin had a higher number of transferable embryos (p < 0.005), fewer embryos classified as degenerated (p < 0.001) and fewer TUNEL‐positive cells/blastocyst (p < 0.001). The number of transferable embryos was positively correlated with E2 concentrations on day of oestrus (r = 0.562; p < 0.01) and P4 concentrations on the day of embryo collection (r = 0.912; p < 0.001). We concluded that in vivo leptin administration during FSH treatment improved embryo quality and affected ovarian steroidogenesis in superovulated goats. 相似文献
6.
Nasopharyngeal cryptococcosis 总被引:1,自引:0,他引:1
R MALIK P MARTIN DI WIGNEY DB CHURCH W BRADLEY CR BELLENGER WA LAMB VR BARRS S FOSTER S HEMSLEY PJ CANFIELD DN LOVE 《Australian veterinary journal》1997,75(7):483-488
Naturally occurring cryptococcosis in five cats, a dog and a koala is described. Involvement of the nasopharynx was documented in all patients, and nasopharyngeal mass lesions accounted for the major presenting complaints in four. Signs referable to nasopharyngeal disease included snoring, stertor, inspiratory dyspnoea and aerophagia. Diagnoses were made by caudal rhinoscopy using a retroverted flexible endoscope, vigorous orthograde flushing with saline, or at necropsy. Concurrent cryptococcal rhinitis was present in all cases, although involvement appeared limited to the caudal nasal cavity in most cases. Typical signs of nasal cavity disease, such as sneezing and nasal discharge, were often absent. Treatment of nasopharyngeal cryptococcosis should include physical dislodgement or debulking of lesion(s) to provide immediate alleviation of upper airway obstruction, followed by systemic antifungal therapy to eliminate residual infection from the nasal cavity. Infections caused by Cryptococcus neoformans var gattii accounted for a disproportionately large number of these cases. 相似文献
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Summary The number of effective factors controlling the inheritance of a quantitative character can be estimated by analysis of F3 lines where the parental lines are homozygous. The procedure is illustrated with data from a study of pod length inheritance in common bean (Phaseolus vulgaris L.). The mean pod length of Sprite, the parent with longer pods, was compared statistically with the means of F3 progenies derived from F2 plants obtained from the appropriate tail of the F2 frequency distribution for pod length. The data indicate that analysis of F3 progenies avoids much of the underestimation of the effective factor number which is characteristic of other procedures. The number of effective factors was estimated to be a minimum of 4 and the correlation between random F2 parents and F3 progeny means was r=0.82.Florida Agricultural Experiment Station Journal Series No. 406. 相似文献
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Summary An experiment was conducted to develop a method for monoploid seedling detection which is more efficient than making cytological examinations of twin seedlings. A recessive seedling marker gene (basal stem color) was used to detect parthenogenesis in female plants. Out of 10 seedlings showing the marker character, 9 were monoploid as expected. The frequency of parthenogenesis among single seedlings was approximately 1 seedling in 2,400. The marker gene technique increases the efficiency of cytological examination from approximately 1 or 2% to 90%.Florida Agricultural Experiment Station Journal Series No 3681 相似文献
9.
Mark J. Bassett 《Euphytica》2005,141(1-2):139-145
The inheritance of the virgata pattern of partly colored seed coats found in common bean (Phaseolus vulgaris L.) Early Giant (EG) was studied by a series of test crosses with line 5-593 and genetic stocks developed by backcrossing selected genes into the recurrent parent 5-593, a Florida dry bean breeding line with a self-colored, black seed coat with genotype T Z Bip P [C r] J G B V Rk. Analysis of the F2 from the cross EG × 5-593 led to the hypothesis that the virgata pattern of EG has genotype t z bipvgt, where vgt stands for virgata. The test cross EG × t z virgarcus BC3 5-593 confirmed the hypothesis that EG carries t z from data recorded in F1, F2, and 27 F3 progenies from randomly selected F2 plants. The F3 segregation was also consistent with the hypothesis that a single recessive gene converts virgarcus into virgata. The test cross EG × t z bip bipunctata BC3 5-593 failed to show genetic complementation in F1 progeny, and the F2 segregated 3:1 for the parental phenotypes virgata and bipunctata, respectively. Including previously published data, all possible crosses were made among bipunctata, virgata, and virgarcus parents, supporting a multiple allelic series at Bip. We propose the gene symbol bipvgt for the new allele at Bip, where the allelic series has the order of gene dominance Bip > bipvgt > bip. Based on test crosses, the complete seed-coat color and pattern genotype of EG is tz bipvgt P [C r] J G B vlae rkd. 相似文献
10.
Previous predictions of sea-level change subsequent to the last glacial maximum show significant, systematic discrepancies between observations at Tahiti, Huon Peninsula, and Sunda Shelf during Lateglacial time (approximately 14,000 to 9000 calibrated years before the present). We demonstrate that a model of glacial isostatic adjustment characterized by both a high-viscosity lower mantle (4 x 10(22) Pa s) and a large contribution from the Antarctic ice sheet to meltwater pulse IA (approximately 15-meters eustatic equivalent) resolves these discrepancies. This result supports arguments that an early and rapid Antarctic deglaciation contributed to a sequence of climatic events that ended the most recent glacial period of the current ice age. 相似文献