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排序方式: 共有377条查询结果,搜索用时 15 毫秒
1.
山羊传染性胸膜肺炎支原体和绵羊肺炎支原体对抗菌药物敏感性的研究 总被引:4,自引:0,他引:4
测定了环丙沙星、氧氟沙星、单诺沙星、红霉素、罗红霉素、泰乐菌素、泰妙菌素、四环素等8种药物对羊肺炎支原体两个标准株Y-98和Y-goat的体外抑菌浓度以及红霉素与氧氟沙星、泰乐菌素对Y-goat和四环素与氧氟沙星、泰乐菌素对Y-98的联合药敏作用.结果表明,这8种抗菌药物对Y-goat和Y-98的MIC(μg/mL)分别为:环丙沙星0.223、0.002 23,氧氟沙星0.281、0.014 0,单诺沙星0.136、0.014 0,红霉素0.021 8、无效,罗红霉素0.032 7、无效,泰乐菌素0.042 2、0.039 0,泰妙菌素0.021 7、0.052 0,四环素0.195、0.052 0.红霉素与氧氟沙星的联合药敏指数为1,是相加作用;红霉素与泰乐菌素对Y-goat的联合药敏指数为1.5,是无关作用;四环素与氧氟沙星、泰乐菌素对Y-98的联合药敏试验指数均为0.375,是协同作用. 相似文献
2.
In vitro studies of Norwegian Red bovine semen immobilized and cryopreserved in alginate solid gel network
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AH Alm‐Kristiansen ER Gaustad G Bai FB Standerholen G Klinkenberg E Kommisrud KE Waterhouse 《Reproduction in domestic animals》2018,53(2):365-370
Development of new semen cryopreservation techniques improving sperm survival and ensuring availability of viable spermatozoa for a prolonged time‐period after AI is promising tools to reduce sensitivity of timing of AI and enhance overall fertility. The SpermVital® technology utilizes immobilization of bull spermatozoa in a solid network of alginate gel prior to freezing, which will provide a gradual release of spermatozoa after AI. The objective of this study was to compare post‐thaw sperm quality and in vitro sperm survival over time of Norwegian Red bull semen processed by the SpermVital® (SV) technology, the first commercialized production line of SpermVital® (C) and by conventional procedure applying Biladyl® extender (B). Post‐thaw sperm motility was not significantly different between SV, C and B semen (p > .05). However, sperm viability and acrosome intactness were higher for SV than C and B semen (p < .05). Small differences in DNA quality were observed (p < .05). Sperm viability after storage in uterus ex vivo was higher for SV than for C semen (p < .05). Furthermore, sperm survival in vitro over time at physiological temperature was significantly higher for SV semen than C semen as well as B semen during the incubation period of 48 hr (p < .05). In conclusion, the SpermVital® technology is improved and is more efficient in conserving post‐thaw sperm quality and results in higher sperm viability over time in vitro for SV than for C and B semen. 相似文献
3.
Intact spinach chloroplasts were used to determine if clomazone, 5-OH clomazone, and/or 5-keto clomazone inhibited the chloroplastic isoprenoid pathway. When isopentenyl pyrophosphate was used as a precursor, neither clomazone nor the clomazone metabolites (5-OH clomazone and 5-keto clomazone) inhibited the formation of products separated by HPLC in the organic phase. However, when pyruvate, a substrate for the first committed step of the pathway, was used as a precursor, both 5-keto clomazone and fosmidomycin reduced the formation of a non-polar product and increased the formation of a polar product in the organic phase. Only 5-keto clomazone, not 5-OH clomazone or clomazone, inhibited the formation of an additional product other than fosmidomycin in the aqueous phase from pyruvate incorporation. In an in vitro assay, 5-keto clomazone inhibited DXP synthase, the enzyme catalyzing the first committed step of the chloroplastic isoprenoid pathway. Therefore, our studies show that neither clomazone nor 5-OH clomazone inhibits the chloroplastic isoprenoid pathway, only 5-keto clomazone does. 相似文献
4.
Alejandro Merchan Elizabeth J. Barrett Dwayne H. Rodgerson 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(1):22
A technique for hand-assisted laparoscopic removal of cystic calculi in male horses is described. Three cystic calculi ranging from 5 to 10 cm in diameter were removed successfully using bilateral para-inguinal laparoscopic portals assisted by a human hand within the abdomen, effectively sealing the midline incision. Long-term follow-up information obtained from referring veterinarians and owners indicated that the patients returned to their previous activity level and had no recurrence of clinical signs in the following 6 to 12 months.Key clinical message:Hand-assisted recumbent laparoscopy is a satisfactory surgical technique for removal of cystic calculi in horses which cannot be operated on standing or whose body condition would make the parainguinal approach difficult. 相似文献
5.
6.
Effect of Pimobendan in Dogs with Preclinical Myxomatous Mitral Valve Disease and Cardiomegaly: The EPIC Study—A Randomized Clinical Trial
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A. Boswood J. Häggström S.G. Gordon G. Wess R.L. Stepien M.A. Oyama B.W. Keene J. Bonagura K.A. MacDonald M. Patteson S. Smith P.R. Fox K. Sanderson R. Woolley V. Szatmári P. Menaut W.M. Church M. L. O'Sullivan J.‐P. Jaudon J.‐G. Kresken J. Rush K.A. Barrett S.L. Rosenthal A.B. Saunders I. Ljungvall M. Deinert E. Bomassi A.H. Estrada M.J. Fernandez Del Palacio N.S. Moise J.A. Abbott Y. Fujii A. Spier M.W. Luethy R.A. Santilli M. Uechi A. Tidholm P. Watson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(6):1765-1779
7.
Sorenmo K Samluk M Clifford C Baez J Barrett JS Poppenga R Overley B Skorupski K Oberthaler K Van Winkle T Seiler G Shofer F 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(6):1347-1354
BACKGROUND: Canine splenic hemangiosarcoma (HSA) is a fatal malignancy, and most affected dogs die within a few months of diagnosis. Most dogs present with signs from tumor rupture, resulting in hemoabdomen and intra-abdominal dissemination. The abdomen is also the main site of disease recurrence. HYPOTHESIS: Intraperitoneal (IP) administration of doxorubicin will delay or prevent intra-abdominal tumor recurrence and prolong survival in dogs with HSA. ANIMALS: Fourteen dogs with splenic HSA. METHODS: A prospective, unmasked, uncontrolled clinical trial. After staging of disease status and splenectomy, pegylated liposomal encapsulated doxorubicin was administered intraperitoneally (1 mg/kg body weight) every 3 weeks for 4 cycles. All dogs were monitored for recurrence of HSA. Samples of plasma and abdominal fluid were collected for measurement of doxorubicin concentration and pharmacokinetic analysis. Nonlinear mixed-effect modeling was used to describe the pharmacokinetics of liposomal doxorubicin administered IP. RESULTS: All 14 dogs died, 12 because of HSA and 2 from other causes. Postmortem examination was performed on 12 dogs. All 12 dogs died because of HSA-related causes and had hepatic metastases and hemoabdomen. The IP-treated dogs had fewer serosal, mesenteric, and omental metastases than historical controls treated with systemic doxorubicin. Results of the postmortem examination and pharmacokinetic analysis confirmed that IP delivery of doxorubicin resulted in an effective drug concentration with a clearance comparable with that after i.v. delivery. CONCLUSIONS AND CLINICAL IMPORTANCE: IP pegylated liposomal encapsulated doxorubicin administration did not prevent intraabdominal recurrence of HSA in dogs. 相似文献
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9.
Contrasting soil management techniques were applied to a hardsetting red-brown earth (Alfisol) used for flood-irrigated wheat (Triticum aestivum) production at Trangie, N.S.W., Australia. The individual and combined effects of deep mouldboard ploughing to a depth of 0.45 m, gypsum application (5 t ha−1) and double cropping upon aggregate stability, bulk density, porosity, cone index and the non-limiting water range were evaluated. Dispersion and slaking of the surface soil were unaffected by the treatments when measured at the end of the second year of the experiment. Approximately 60% of the soil mass in the 0–0.15 m layer slaked on wetting, whereas less than 1% of the soil dispersed. Organic carbon (OC) levels of the surface soil were not affected by double cropping or gypsum application, but were reduced by mouldboard ploughing from 0.9% to 0.6% OC. The relationship between OC and macroaggregate (more than 250 μm) stability indicated that large increases in OC beyond 0.7% OC were required for relatively small increases in aggregate stability. Mouldboard ploughing increased clay content of the upper 0.15 m of the soil from 22% to 27%. This was associated with an increase in the frequency and depth of cracking which, however, diminished over time. The non-limiting water range (NLWR) was expanded in the uppermost 0.1 m by gypsum application from 0.15–0.30 to 0.09–0.28 m3 m−3. Mouldboard ploughing expanded the NLWR at a depth of 0.2 m. Penetrometer resistance, on average, exceeded the critical value for wheat root growth at a water content of 0.15 m3 m−3, which is substantially higher than the wilting point (0.09 m3 m−3). Excessive resistance to penetration as opposed to inadequate aeration or water availability is the main agronomic impediment in these soils, at least in the initial stages of crop development. Penetration resistance within the 0.05–0.3 m layer was reduced during a drying cycle in the order: mouldboard ploughing>gypsum>double cropping. The reduced penetration resistance associated with mouldboard ploughing was due to higher water content to a depth of 0.2 m and reduced bulk density below this depth. 相似文献
10.
Signs of ascites were observed in only nine of 1863 cattle examined over a period of five years. The ascites was most commonly associated with either primary or secondary cardiac disease; cattle with ascites have a poor prognosis because the condition is usually assocaited with terminal disease. Two animals had cor pulmonale with chronic pneumonia, three had cardiomyopathy, one had bacterial endocarditis, two had thrombosis of the caudal vena cava and one had diffuse abdominal epithelioid mesothelioma. 相似文献