莫能菌素浓度对混合瘤胃微生物体外发酵的影响

本试验研究了莫能菌素对混合瘤胃微生物体外发酵的影响 ,以期确定莫能菌素在瘤胃内容物中的适宜添加浓度。混合瘤胃微生物由饲喂花生秧基础日粮的阉公牛瘤胃内容物和瘤胃液制备 ,用简易人工瘤胃装置测定莫能菌素浓度对产气量、瘤胃乙丙酸比例、粗纤维降解率和真蛋白产量的影响。结果表明 ,莫能菌素浓度强烈地抑制产气量 ,两者呈线性负相关(P<0.05);粗纤维降解率开始时随莫能菌素浓度的升高而增加 ,在浓度为0.078mg/L时达到最大值 ,两者呈二次幂函数关系 ;添加莫能菌素降低了瘤胃乙丙酸比例 ,但瘤胃乙丙酸比例随莫能菌素浓度的提高有逐渐增加的趋势 ;在莫能菌素浓度为0.1～2.0mg/L的范围内 ,添加莫能菌素对蛋白质的产量没有明显影响     

茶黄素类抗肿瘤作用研究进展

简述了茶黄素类(Theaflavins，TFs)的化学结构，并从抗肿瘤、抗炎、抗氧化、抗病毒和抗心血管疾病五个方面综述了茶黄素类的药理作用，其中从肿瘤的发生、发展两个阶段着重分析了茶黄素类的作用机理，最后提出了TFs作为药品开发所存在的问题。     

鸡脑脊髓炎高免卵黄抗体的制备及治疗试验

鸡脑脊髓炎是由鸡脑脊髓炎病毒引起的主要侵害雏鸡的一种传染病 ,其主要特征是共济失调 ,头颈部震颤和非化脓性脑炎 ,近年来河南许多鸡场有本病的发生 ,为找到本病的有效治疗措施 ,我们开展了此项研究 ,报告如下。1　材料与方法1 .1　试验鸡　选择健康蛋用鸡 50只 ,经检查没有白痢等垂直传播的疾病 ,由郑州牧专微生物教研室饲养。1 .2　疫苗　鸡 AE弱毒苗 ,购自河南兽医防治站 ,鸡AE油乳剂灭活苗 ,由郑州牧专微生物教研室自制。1 .3　鸡 AE琼扩抗原及阳性血清　由郑州市畜牧工作总站提供。1 .4　试验鸡免疫　一免 ,每只鸡分别肌注 AE弱…     

耕牛血吸虫病检测方法比较试验

目的为了解几种检测耕牛血吸虫病的敏感性进行本试验。方法将经粪便毛蚴孵化法检出血吸虫病阳性的水牛(6头)，同时进行干卵聚酯薄膜环卵沉淀试验、间接血凝试验及快速诊断试纸盒检验的测试。结果以其检测结果与粪便毛蚴孵化法相比，阳性符合率分别为100％、100％和83％，3种方法间无显著差异。结论快速诊断试纸盒检测法以其操作简便且准确、快速、特异性高的特点，在动物血防普查工作中具有广阔的应用前景。     

猪血浆蛋白(酶)多态性与杂种优势的关系

为探讨血浆蛋白 (酶 )多态性与杂种优势的关系 ,测定了杜洛克、长白、大白、杜×长大、大×长大、长×大、大×长共 7个品种 (组合 )的 8个血浆蛋白 (酶 )位点的多态性及部分生长和胴体性状 ,计算了平均基因杂合度与部分经济性状实测值和杂优率的相关关系 .结果表明 ,平均基因杂合度与遗传距离呈正相关 ,与日增重、屠宰率、背膘厚、后腿比例的实测值或杂优率呈正相关 ,与眼肌面积的实测值和杂优率呈负相关 .平均基因杂合度和亲本间遗传距离可为预测杂种优势提供依据 .     

水分管理方式对香根草生长发育的影响

对四种不同的水分管理条件下香根草生长研究发现：香根草最适宜在土壤水分饱和的湿润环境中生长,这时它的成活率最高,分蘖速度最快,植株高度生长也最快,定植后3个月,其对应的根体积、根干生物量、茎叶干生物量和根茎比也最高。     

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.     

Effect of mutant β-catenin gene on the proliferation of hepatocytes

AIM:The β-catenin is a key molecule in the Wnt signal pathway, which plays a critical role in normal development and tumorigenesis. However, the mechanisms of the β-catenin on the cell growth control are still not completely defined. The aim of this study was to test the hypothesis that the mutant β-catenin may regulate the hepatocyte proliferation. METHODS: The immortalized murine hepatocyte cell line, AML12, was used for this study. A plasmid that contain mutant β-catenin S33Y was transfected into the AML12 cells and a stable cell line AML12S33Y was established. The cell growth property of this cell line and the parental cell were compared by flow cytometry analysis and direct cell count. The cells were also tested for the ability to form soft agar colonies, and the ability to form tumors in the severe immune deficient mice (SCID). RESULTS:1. The mutant β-catenin containing cell line AML12S33Y has higher proliferating index compared with the parental AML12 cells (P<0.01), suggesting that mutant β-catenin promotes cell growth. 2. The mutant β-catenin cells formed small colonies in soft agar after 4 weeks of culture, but did not generate tumor in SCID mice. CONCLUSION:The mutant β-catenin promotes liver cell growth.     

Effect of antifibrotic herb serum on rat hepatic stellate cell proliferation and collagen synthesis

AIM: To investigate the effect of Chinese herbs, Ganxianfang(GXF), on rat hepatic stellate cells (HSC) proliferation and collagen synthesis. METHODS: Two types of herb serum, portal venous serum and circumferential venous serum, were prepared from rats infused intragastrically with 16, 8, 4 times adult dose of GXF decoction. HSC isolated from rat liver were processed with the above sera in vitro. Then we mensurated the radioactivity of HSC admixed with [[³H]H]proline and [[³H]H]thymine to judge the effect on proliferation and collagen synthesis of HSC. RESULTS: Both two types of serum collected 0.5, 1, 2 h after intragastrical infusion inhibited HSC proliferation (P＜0.05), and the serum collected 1 h after intragastrical infusion had the strongest effect (P＜0.05). Portal serum decreasea collagen synthesis (P＜0.05), but circumferential serum had no effect (P＞0.05). CONCLUSION: Inhibition of HSC proliferation and decrease of collagen synthesis may contribute to the GXF antifibrotic action.