排序方式: 共有30条查询结果,搜索用时 15 毫秒
1.
2.
Melatonin reduces apoptotic cells,SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts
下载免费PDF全文
![点击此处可从《Reproduction in domestic animals》网站下载免费的PDF全文](/ch/ext_images/free.gif)
TC Marques EC da Silva Santos TO Diesel LO Leme CF Martins MAN Dode BG Alves FPH Costa EB de Oliveira ML Gambarini 《Reproduction in domestic animals》2018,53(1):226-236
Effects of adding different concentrations of melatonin (10?7, 10?9 and 10?11 M) to maturation (Experiment 1; Control, IVM + 10?7, IVM + 10?9, IVM + 10?11) and culture media (Experiment 2; Control, IVC + 10?7, IVC + 10?9, IVC + 10?11) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10?9 M) from Experiments 1–2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM + 10?9, IVC + 10?9, IVM /IVC + 10?9). In Experiment 1, maturated oocytes from Control and IVM + 10?9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC + 10?7 (43.5%; 56.7%) and IVC + 10?9 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC + 10?9 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC + 10?9 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM /IVC + 10?9 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC + 10?9 treatment also had a higher mRNA expression of antioxidant gene (SOD 2) compared to the Control, as well as the heat shock protein (HSPB 1) compared to the IVM + 10?9. Reactive oxygen species production was greater in the IVM /IVC + 10?9 treatment group. In conclusion, the 10?9 M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality. 相似文献
3.
4.
5.
6.
Quadratic fitting was used to regress semen characteristics of 1441 samples consisting of 12‐month collection from 58 Duroc boars against animal age varied from 10 to 80 months. Data was divided into two groups of cool (14.0–22.7°C, RH 81.5%) and hot season (22.9–29.9°C, RH 86.6%), to test effects of age, season and their interactions. Results revealed that young boars of around 1 year old could endure the hot season. The endurance gradually diminished as animals grew. In the hot season animals exhibited peak performance at age around 33 month and it remained for 1 month, while cool‐season kept boars could last for 48 months from 16 months old onward. The reproductive longevity should be 51 month in a subtropical environment and it may extend to 70 month if heat stress can be avoided. The estimated total sperm contribution of a Duroc boar would be 1.8 times more when kept below 22°C than in a natural subtropical environment. It is concluded that to maintain Duroc boars as semen donor to at least 4 years of age is feasible in a subtropical environment and boar longevity could reach 6 years old if well kept in a temperate region. 相似文献
7.
笋用竹害虫天敌资源研究 总被引:1,自引:0,他引:1
本文报道了浙江省笋用竹害虫天敌资源调查的结果,共查明天敌资源44种,经鉴定属于螳螂目1科2种,半翅目1科2种,蛇蛉目1科1种,脉翅目1科2种,鞘翅目2科9种,双翅目2科2种,膜翅目9科22种;蛛形纲蜱螨目1科1种,蜘蛛目3科3种,指出了天敌的寄主和分布。 相似文献
8.
9.
Etelvina M. C. G. Nunes Maria M. G. Pereira Amilton P. R. Costa Mara N. B. A. Araripe Rodrigo M. Calvet Carina M. Pereyra Maria L. X. Azevedo Raizza E. E. Pinheiro Verbena C. Alves Airton M. Conde Jnior Lidiane S. N. Ramos Joao B. Lopes Camila G. Marinho Mara C. S. Muratori 《International Aquatic Research》2019,11(1):73-83
The detection of mycotoxins in feeds and their ingredients in aquaculture gained prominence due to losses caused in production and animal health, mainly the occurrence of aflatoxin (AFB1). The aim of this study was to evaluate the effects of AFB1 on the performance of tambaqui fingerlings (Colossoma macropomum). Four hundred tambaqui were used. Four different treatments were evaluated: treatment T1, considered as the control treatment (CT) with 3.84 μg kg−1; treatment T2, treatment T3 and treatment T4 with 500, 1000 and 2000 μg kg−1 of AFB1, respectively. The AFB1 of the samples (muscle, liver and kidney) was detected by high-pressure liquid chromatography. Four fingerlings from each treatment for histological analysis were examined. Moreover, the performance parameters (weight gain, feed conversion and feed intake) were studied. The levels of toxins used in T2, T3 and T4 represent a reduction in the growth of 14%, 35% and 45%, respectively. The T3 and T4 showed the lowest weight gain (78%) and the worst feed conversion. Aflatoxin B1 in muscle (3.28 μg kg−1) and kidneys (8.8 μg kg−1) in the T3, as well as liver (4.4 μg kg−1) and kidney (4.08 μg kg−1) in T4, was detected. Histopathological changes in liver and kidney tissues of fingerlings were more pronounced in T3 and T4. Fingerlings that consume feed contaminated with AFB1 in concentrations higher than 500 μg kg−1 present decreases in growth, reduction in weight gain and feed intake with increased feed conversion. The consumption of feed contaminated with 1000 and 2000 μg kg−1 of AFB1 caused severe deterioration in the hepatic and renal tissues. 相似文献
10.