A review of okara (soybean curd residue) utilization as animal feed: Nutritive value and animal performance aspects

Year by year, huge quantities of by-products are generated during the manufacturing process of soybean-based products. Okara is one of the by-products, and it is an insoluble portion of the soybean. It consists of high moisture (8.4–22.9%); on dry matter basis, it contains high metabolizable energy (9.0–14.2 MJ/kg) and other components that include crude protein (20.9–39.1%), crude fiber (12.2–61.3%), crude fat (4.9–21.5%), and ash (3.4–5.3%). Fermentation of okara improves its nutritional quality and reduces its anti-nutrient contents. Due to animals' palatability, okara can be used to replace the soybean meal/concentrate feed partially or completely in ruminant's diet and partially in nonruminant's diet. Okara feeding does not depress the intake, digestibility, growth, milk production, blood metabolic profiles, and meat quality of animals. However, this by-product decays quickly due to its high moisture content, and its heavy weight and sticky nature make it difficult to process and expensive to dry using conventional methods. This paper thoroughly summarizes the utilization of okara as animal feed in the cause of developing a general guideline with favorable levels of inclusion in the diets of animals for its exploitation and valorization. This review will encourage further research to develop eco-friendly and value added feed for animals.     

REVIEW: Properties of Cereal Brans: A Review

Cereal brans are functional ingredients with high nutritive value and enormous health properties. Cereal brans have not been fully utilized in food systems despite their health benefits. This review presents an overview on the physical, chemical, microbiological, functional, and sensory properties of cereal brans for possible comparisons and selection to enhance the utilization of this underutilized milling fraction.     

Epidemiologic investigation of an outbreak of goose parvovirus infection in Sweden

An outbreak of goose parvovirus (GPV) infection on a Swedish goose farm in the spring of 2004 increased the mortality rates from 2% in the early unaffected hatches to 90% and 99% respectively in the two hatches following virus introduction and 40% in goslings hatched later in the same breeding season. In this paper we describe the clinical observations, diagnostic procedures, and epidemiologic investigation carried out to elucidate the source of the infection. The diagnosis was confirmed by serology, virus isolation, and sequence analysis of a 493-bp-long fragment of the VP1 gene. Phylogenetically the causative virus was closely related to pathogenic GPV strains isolated in 2003 and 2004 from Poland and the United Kingdom, respectively. The Swedish isolate exhibited less homology with pathogenic strains from Hungary and Asia and with attenuated vaccine strains. The epidemiologic investigation showed that the virus was first introduced to a contract farm (farm A) and then was transferred with newly hatched goslings to the farm that had submitted the birds for necropsy (index farm). The exact time and source of the virus introduction to farm A could not be determined with absolute certainty. Possible sources of the infection included backyard goose eggs that had been delivered to farm A for subcontract incubation and hatching, wild geese that frequented the flock of breeding geese on pasture on farm A, and a clutch of Canada goose eggs (Branta canadensis) that had been produced by wild geese and was hatched in the same machine as the eggs produced by farm A.     

Effect of thermal stress on Hsp70 gene expression and female reproductive performance of giant freshwater prawn,Macrobrachium rosenbergii

Using Hsp70 as a biomarker, thermal stress impinges on reproductive organs, ovary and hepatopancreas were being analyzed by determining the expression of Hsp70 mRNA inside the organs after the adult inter‐molt females were subjected to thermal treatment at 35, 30 and 28°C (Control). Results showed the expression of Hsp70 mRNA under thermal treatment of 35°C after 2 hr recovery in ovary were upregulated at 2, 4, 6, 12, 24 hr and 30 days compared to control whereas in hepatopancreas under similar treatment, the expression of Hsp70 mRNA were significantly higher than control at 6, 24 hr and 30 days. Frequency of reproductive molt at 35°C showed the ovary of females were failed to develop and only entered common molt along three consecutive molt cycles. For 30°C thermal treatment, the expression of Hsp70 mRNA was significantly higher than control after 2 hr recovery but returned to normal afterwards until 30 days’ thermal treatment. Maternal heat shock for 2 hr at 35°C were found to give significantly lower frequency of reproductive molt and longer duration of ovarian development and incubation period whereas maternal heat shock for 2 hr at 30°C gave lower frequency of reproductive molt, slower development of embryo and lower hatching success compared to untreated control. This study suggests that short and long‐term thermal stress at 30 and 35°C were found to affect the induction of Hsp70 mRNA in reproductive organs of Macrobrachium rosenbergii and also influence their reproductive performance.     

Meat and Bone Meal as Partial Substitute for Fish Meal in Nursery Diet for Giant Freshwater Prawn, Macrobrachium rosenbergii (de Man)

An experiment was conducted for 60 d in a recirculatory system consisting of 20 glass aquariums (each of size 41 × 41 × 46 cm) to evaluate meat and bone meal (MBM) as partial replacement for fish meal (FM) in Macrobrachium rosenbergii postlarvae (PLs) with a view to develop an inexpensive nursery diet. Three nursery diets were formulated to contain 32% protein and to be as isoenergetic as possible. FM in these diets was progressively replaced with MBM (Diet 1 contained 25% FM and 14% MBM, Diet 2 contained 20% FM and 20% MBM, and Diet 3 contained 15% FM and 26% MBM). Inclusion levels of mustard oilcake and sesame meal in diets were kept fixed at 10%. A commercial shrimp nursery diet (30% protein) from Saudi Bangla Fish Feed Ltd., Mymensingh, was used as the reference diet (Diet 4). Each treatment had three replicates. PLs of M. rosenbergii (26 ± 0.02 mg) were stocked at the rate of 60 PLs (1 PL/L) in each aquarium. At the beginning, PLs were fed three times daily at a rate of 20% of the body weight and it was gradually reduced to 10% at Day 30. The ranges of water quality parameters in the system were as follows: temperature, 27–29 C; dissolved oxygen, 6.5–8.4 mg/L; pH, 6.8–8.3; and total ammonia, 0.01–0.15 mg/L. The result of the study showed that there was no significant difference (P > 0.05) between the weight gains of PLs fed Diet 1 (containing 14% MBM) and Diet 4 (reference diet) but was significantly higher than other dietary groups containing higher levels of MBM. Diets 1 and 4 showed significantly lower (P < 0.05) apparent food conversion ratios (1.74 and 1.73, respectively). The survival (%) of PLs ranged between 62 and 76% with PLs fed Diets 1 and 4 showing significantly higher (P < 0.05) survival. The result of the study demonstrated that the MBM could be included at 14% level in a nursery diet, and a diet containing 25% FM, 14% MBM, 10% sesame meal, and 10% mustard oilcake may be recommended for profitable production of PLs of M. rosenbergii.     

Characteristics of the organic matter in a slightly and in a severely crusted soil

Chemical and spectroscopic characteristics were determined on the organic matter in two Luvisolic soils from northwestern Alberta, Canada. One of these (the Debolt soil) is known to crust only slightly while the other (the Demmitt soil) exhibits severe crusting after heavy rainfalls. The Debolt top soil is richer in total C, total N, carbohydrates and proteinaceous materials than the Demmitt top soil. Humic acid extracted from the Debolt soil is more aliphatic, and contains fewer CO₂H groups than does humic acid extracted from the Demmitt soil. The quality of the organic matter appears to play a role in soil crusting.     

Relationship between in vitro production of auxins by rhizobacteria and their growth-promoting activities in Brassica juncea L.

Rhizobacteria were isolated from the rhizosphere of different Brassica species and assayed for their ability to produce auxins in vitro. The isolates varied greatly in their potential for auxin production (ranging from 0.33 to 11.40 µg ml^-1). L-Tryptophan (an auxin precursor) addition to the media increased the auxin production by several fold. Based upon in vitro auxin production and growth promotion of B. juncea seedlings caused by various isolates under gnotobiotic conditions, promising isolates were selected and tested in pot trial to observe their effects on growth, yield and oil content of the same Brassica species. Results showed that seed inoculation with different isolates of rhizobacteria significantly increased plant height (up to 56.5%), stem diameter (up to 11.0%), number of branches (up to 35.7%), number of pods per plant (up to 26.7%), 1,000-grain weight (up to 33.9%), grain yield (up to 45.4%) and oil content (up to 5.6%) over the uninoculated control. Isolate S54 gave the most promising and consistent results. Highly significant correlations between L-TRP-derived auxin production by plant growth-promoting rhizobacteria (PGPR) in vitro and grain yield (r =0.77^**), number of pods (r =0.78^**) and number of branches per plant (r =0.77^**) of B. juncea were found. It was hypothesized that these PGPR may influence the growth and yield of inoculated plants by production of auxins in the rhizosphere of inoculated plants from the L-TRP present in the root exudates, although other mechanisms of action might have also contributed.     

Differentiation of interglycosidic linkages in permethylated flavonoid glycosides from linked-scan mass spectra (B/E)

A series of per-O-methylated flavonoid di- and tri-glycosides, linked with 1-2 and/or 1-6 glycosidic bonds between sugar rings that were isolated from different plant materials were analyzed. It was demonstrated that the fragmentation behavior of permethylated flavonoid glycosides is dependent on the glycosidic bond placement between sugars. Y(n) type fragment ions, created after glycosidic bond cleavage with oxygen retention on sugar at the reducing end for permethylated compounds, were observed in the normal and linked-scan mass spectra recorded for alpha(1-2) bonded conjugates of flavonoid di- and tri-glycosides. Moreover, for alpha (1-6) linked glycosides, Y fragments created after rearrangement and elimination of internal sugar residues were observed in addition to Y(n) type ions, but these fragment ions were not registered in normal desorption ionization spectra. This second type of fragmentation was also reported previously in collision-induced dissociation tandem mass spectrometry (CID MS/MS) spectra of some oligosaccharides and flavonoid glycosides, but their presence was independent of the glycosidic bonds placement between sugar rings.