寄主和非寄主种子分泌物中游离氨基酸和可溶性糖含量及其对大豆疫霉的作用

采用HPLC技术分析大豆疫霉抗病品种Williams 82、感病品种Sloan及非寄主菜豆紫花油豆、玉米绥玉23种子分泌物中游离氨基酸和可溶性糖种类和含量,测定相对原位浓度氨基酸混合液和糖混合液对大豆疫霉菌丝生长、卵孢子形成及游动孢子趋化性的影响。结果表明,不同作物品种种子分泌物中氨基酸和糖的种类及含量差异显著。其中,大豆抗病品种种子分泌物中氨基酸和可溶性糖含量显著高于感病品种Sloan和其他两种非寄主作物。对于菌丝生长,除Williams 82氨基酸处理表现为抑制外,其他处理均有促进。对于卵孢子形成,氨基酸处理表现为紫花油豆=Williams 82>Sloan=绥玉23,可溶性糖处理表现为紫花油豆>Sloan>Williams 82>绥玉23。对于游动孢子趋化性,表现为Sloan>紫花油豆>Williams 82>绥玉23。4个品种种子分泌物差异主要表现在对游动孢子差异性趋化作用上,表明种子分泌物中游离氨基酸和可溶性糖在一定程度上作为信号分子参与大豆疫霉寄主选择性和寄主抗病性,研究为防治大豆疫霉根腐病提供理论依据。     

三种侵染百香果的马铃薯Y病毒属病毒外壳蛋白比较

<正>0 Introduction The family Potyviridae accounts for approximately 30%of know n plant viruses, infecting an extensive range of dicots and monocots and causing enormous damage in agriculture^[1]. The genus Potyvirus is the largest genus in this family and all members of the Potyvirus share similar common characteristics and a same genome organization. Virions of potyviruses are non-enveloped,flexuous,