CdSe/ZnS核壳型量子点标记朊蛋白的研究

利用组氨酸与锌离子的特异性结合,用水溶性CdSe/ZnS核壳型量子点标记朊蛋白(PrPC).研究了量子点标记朊蛋白的荧光光谱、琼脂糖凝胶电泳以及二者结合比.标记后荧光强度增加两倍多,二者结合比为3∶1.这种方法能快速简便地实现朊蛋白的定位点标记,为进一步深入研究朊蛋白提供了一种新的标记手段.     

ZnS-AgInS2型量子点的水溶性修饰

采用二巯基辛酸修饰ZnS-AgInS2型量子点,建立了一种制备水溶性量子点的方法,得到了性能优良的水溶性量子点.该修饰方法简便易行,具有较高的转化率;得到的量子点荧光性能损失较小,可以稳定放置6个月.     

金属离子对二巯基辛酸修饰CdSe/ZnS量子点荧光的无规则影响

以二巯基辛酸为修饰试剂制备了水溶性CdSe/ZnS量子点.报道了Pb~(2+),Cu~(2+),Cd~(2+)和Ag~+对DHLA-QDs荧光的无规则影响.初步探讨了金属离子对二巯基辛酸修饰的CdSe/ZnS量子点荧光强度无规则影响的机理,推测量子点能否用作金属离子探针与其表面修饰基团密切相关.

Abstract：

Water-soluble dihydrolipoic acid capped CdSe/ZnS quantum dots (DHLA-QDs) were prepared by a slight modification of the procedure of precursors. DHLA-QDs are widely used in biological labeling for Water-soluble and biocompatible. Herein, we reported that Pb~(2+) , Cu~(2+) , Cd~(2+) and Ag~+ had great effects on the fluorescence of the DHLA-QDs, but these effects were ruleless. It was speculated that the modified ligands on the surface of QDs played an important role in whether water-soluble QDs were suitable to detect metal ions as fluorescence probes.The possible reason was discussed.