Cloning of plasma membrane H-ATPase gene in Populus euphratica Oliv.

For this paper, the plasma membrane (PM) H -ATPase gene has been cloned from Populus euphratica Oliv. through a homology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame (ORF) which encodes a putative H -ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H -ATPase of Primus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H -ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeⅠ, FbaⅠand BglⅡ, and Southern blot.     

果树覆膜结合贮肥水穴技术

<正>山地修成复式梯田的果园,根据当地条件,单株或几株一起覆盖地膜。覆膜前要精细整地,除去根蘖残茬、石块,打碎大土块。从树干周围向外围稍有坡度,呈中间高四周较低的弓形。在每株树外围不同方向挖4~6个穴,直径20~30 cm,深不超过40 cm。然后装入经尿液浸泡过的草把,填土时混入过磷钙和尿素,每穴50~100g。穴顶留一小洼,然     

苹果建园幼树栽植管理技术要点

正甘肃省正宁县最适宜栽培的苹果品种是红富士系的长富2号、秋富1号、寿红,烟富1号、烟富3号、烟富6号等,搭配品种是秦冠、新红星、金冠、王林。建园栽植技术如下。(1)栽植时间。分春栽和秋栽,春栽宜在土壤解冻后树苗发芽前进行,以3月中下旬最好。秋栽宜在树     

湫头镇苹果发展现存在问题及对策

正湫头镇地处甘肃省正宁县东南部子午岭的林缘,为优质果带中心区。全镇现有苹果面积1000hm~2,其中盛果期果园653.3hm~2,年产果品29400t,产值1470万元,人均果品收入3000元以上。有气调贮藏库2处,地下贮藏库3处,苹果专业合作社1个。新建乔化密植苹果示范园1处,矮化密植苹果园1处。湫头镇苹果产业发展中存在的问题。(1)果园土     

EFFECT OF HIGH LOADS IN THE BRIDGE LOAD SPECTRUM ON FATIGUE CRACK GROWTH OF 16 Mn STEEL

This paper studies an effect of high loads in Rayleigh distribution random load-ing on fatigue crack growth wi th 16 Mn steel compact tenion specimen,The results show that thehigh loads in the load spectrum close to service load of steel bridges accelerate crack growth and re-duce crack growth life,Lives predicted with constant amplitude da/dn data and equivalent loads arequite in agreement with test lives.     

ELLIPSOMETRIC SPECTROSCOPY OF ELECTROCHROMISM OF POLYANILINE FILMS

The electrochromism of polyaniline films, which is prepared by electropolymer-ization from aniline in acidic aqueous solution onto platinum,is investigated in 330-700nm through in-situ ellipsometric technique. The results indicate that the ellipsometric peak of the reduced state(-0. 2V) of the film appears at 390-400nm and the oxidized state(0. 55V) at 500-530nm. The elec-trochromic reaction from the reduced "yellow" state to the oxidized "green" state is suggested via intermediate state whose ellipsometric peak appears at 466-470nm.     

Complete Genome Sequencing and Analysis of Rehmannia Mosaic Virus Isolate from Shanxi Province

Using double-stranded RNA(dsRNA) technology and sequence-independent amplification(SIA), the molecular identification on infected Rehmannia glutinosa in the field with mosaic symptoms was performed and the whole-genome of the Rehmannia mosaic virus(ReMV) Shanxi isolate(ReMV-SX) was sequenced. Sequencing analysis showed that the virus that infected Rehmannia glutinosa was Rehmannia mosaic virus(ReMV). The full-length of the obtained ReMV-SX sequence(GenBank accession no. JX575184) was 6 395 nt, containing four open reading frames(ORFs). The sequence homology analysis of the complete nucleotide sequence showed that ReMV-SX was 93.8%-97.0% homologous to ReMV in Tobamovirus subgroup I, while only 49.8%-58.9% homologous to the isolates in subgroups II and III of the same genus. Phylogenetic analysis showed that ReMV-SX and ReMV-Henan formed a separate branch and had the closest genetic relationship. The results laid the foundation for ongoing researches in the taxonomic status and evolution of ReMV and for further investigating the pathogenic mechanism of ReMV infecting Rehmannia glutinosa.     

Application of quantum dot-antibody conjugates for detection of sulfamethazine residue in chicken muscle tissue

A new indirect competitive fluorescence-linked immunosorbent assay (cFLISA) method for the detection of sulfamethazine (SM2) in chicken muscle tissue was demonstrated using quantum dots (QDs) as the fluorescence label coupled with secondary antibody. The sensitivity of the cFLISA was compared with that of the HPLC method. The 50% inhibition value (IC50) and the limit of detection (LOD) of the cFLISA were 7.7 and 1.0 ng/mL, respectively. When SM2 was spiked at levels of 50, 100, and 200 ng/g, recoveries ranged from 80.6% to 117.4%, with a coefficient of variation of 6.9-9.6%. In the incurred sample analysis, the amounts of SM2 quantified by cFLISA were similar to the results obtained by the HPLC method. This study shows that cFLISA could be used as a screening method in practice.