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双价抗虫转基因大豆抗苜蓿夜蛾分析(英文) 总被引:4,自引:1,他引:3
[Objective] The aim of the research was to analyze the resistance of binary insect-resistant transgenic soybean to Heliothis viriplaca.[Method]In this experiment, resistance analysis of the stabilized binary insect-resistant transgenic soybean to Heliothis viriplaca was conducted in lab and in field conditions.[Result] The results indicated that the leaves of insect-resistant transgenic soybeans T5-150 and T5-195 showed lighter damage than those of non-transgenic soybeans. Meanwhile, the Heliothis viriplaca larvae fed on leaves of these two transgenic soybeans were characterized by less leaf consumption, shortening survival day, slower development and less pupation.[Conclusion]It was concluded that insect-resistance of transgenic soybean to Heliothis viriplaca was increased dramatically and the research provided a reference for selecting binary insect-resistant transgenic soybean to Heliothis viriplaca. 相似文献
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转CryIA和CpTI双价抗虫基因大豆的获得与稳定表达 总被引:2,自引:0,他引:2
【目的】将抗虫基因转入栽培大豆中,提高大豆的抗食心虫能力。【方法】利用大豆未成熟子叶体细胞胚发生系统高效转化体系,将携带有人工合成的CryIA杀虫基因和CpTI基因的高效双价杀虫基因植物表达载体pGBI121S4ABC转化到大豆主栽品种吉林20与吉林27中。【结果】GUS活性分析、PCR、Southern blot检测证明,目的基因已整合到受体大豆基因组中。T1代转基因大豆抗虫测定表明,转基因材料虫食率比对照显著降低,抗虫能力显著提高。【结论】T3~T5连续3代接虫测定结果表明,转基因大豆株系0-195和0-150的虫食率均比对照低大约30%,其抗虫能力已基本稳定。 相似文献
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