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排序方式: 共有105条查询结果,搜索用时 15 毫秒
1.
从患病缢蛏(Sinvnovacula constricta)中分离到一株病原菌,暂命名为12#菌.对该株的16SrDNA的全序列进行PCR扩增并测序,然后用NCBIBLAST对测序结果进行比对.16S rDNA序列分析表明:12#菌与多株气单胞茵的16S rDNA的同源性均在95%vx以上.在细菌系统分类学中应归属于气单胞茵属.从构建的系统发育树中可以看出:12#茵与点状产气单胞茵(Aeromonas punctata)共同构成一个分支,且该菌株与点状产气单胞菌(Aeromonas punctata)的16S rDNA同源性达到99%以上,由此可以初步认为该菌为点状产气单胞茵(Aeromonas punctata). 相似文献
2.
宁夏引黄灌区为了加强灌区管理,充分体现农户的参与权、知情权、监督权和管理权,在政府的引导下成立了灌区农民用水者协会。农民参与灌溉管理改革的效果越来越明显。协会在农业用水管理中发挥了积极的作用,使宁夏的灌区逐步走向科学、规范的管理模式。 相似文献
3.
Saliva-activated transmission of Borrelia afzelii Canica, Nato, du Merle, Mazie, Baranton et Postic, 1993 was demonstrated using salivary gland extract (SGE) from Ixodes ricinus (L., 1758) ticks and C3H mice. Injection of Borrelia spirochaetes together with SGE increased the level of bacteraemia and accelerated the appearance of bacteria in the urinary bladder, compared with the injection of spirochaetes alone. More I. ricinus nymphs became infected when feeding on mice inoculated with B. afzelii plus SGE. Analysis of cytokines produced by cells of draining lymph nodes from SGE-treated mice showed a suppression of proinflammatory cytokines IFN-gamma, IL-6 and GM-CSF following a transient upregulation in comparison with the control mice infected without SGE. 相似文献
4.
Stefancíková A Stĕpánová G Derdáková M Pet'ko B Kysel'ová J Cigánek J Strojný L Cisláková L Trávnicek M 《Veterinary research communications》2002,26(8):601-611
In the course of epizootological research on Lyme borreliosis in domestic and farm animals, the serological evidence for the occurrence of this zoonosis in cattle was screened. An ELISA showed that 25.2% of cattle from seven geographical areas in Slovakia were positive for anti-Borrelia IgG antibodies. In particular localities, the seroprevalence ranged from 0.6% to 34.3%. Of 33 cases with clinical signs, 20 ELISA-positive samples were also confirmed in Western blots. The most frequent clinical signs were lameness and swollen joints, but most of the cases were asymptomatic. The occurrence of Borrelia burgdorferi antibodies and suspected clinical signs in cattle of Slovak regions indicates that veterinarians should pay attention to this disease in their clinical practice and include it within the differential diagnosis. 相似文献
5.
为提高水利水电工程截流施工过程的施工效率,提出了基于三维图像的可视化仿真方法,利用高效的图形渲染引擎,将仿真结果与可视化系统相结合,为施工系统分析和管理提供了一个有力的工具,为制定合理的施工组织计划提供了科学依据。 相似文献
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该文应用绿量、容量、景观质量(视环境)、使用频率(耐踏率)等绿化评价指标,研究植物群落(种群)空间分布、结构特征与生态效应的关系,探讨了区域范围内生态效应优先,人性空间优先的理论与实践问题,希望通过绿量的探索,寻找植物优化设计,发挥绿化最大综合效益的途径。 相似文献
9.
The construction of
aboveground biomass and biomass model of Dendrocalamus latiflorus plantation in South
Fujian are studied in this paper. The results are showed as follows (1)The aboveground
biomass and the biomass of culm of D.latiflorus may reliably be calculated by the model,
m=a*(D2H)b,but the biomass of its culm and leaf may not. In order
to calculate it, it is necessary to introduce the factor, clear-height,and the
models,m=a*Db(H-h)c and m=a*Db*[(H-h)/h]c.
(2)The total aboveground biomass of D.latiflorus plantation in South Fujian is 39.518 t*hm-2.The
biomass of 3-year-old is the highest,accounting for 59.17 percent of the total, followed
by that of 2-,4- and 5-year-old successively. The biomass of culm is the highest in terms
of organ, accounting for 62.81 percent of the fotal, followed by that of branch and leaf
successively. The total aboveground biomass and the biomass of culm gradually decrease
along the height, and that of branch and leaf gradually decreases from the section of 6~8
m to the top and to the bottom. 相似文献
10.
Senocq D Dupont D Rolet-Répécaud O Faurie F Levieux D 《Journal of agricultural and food chemistry》2001,49(3):1571-1577
To investigate plasmin activity in cheese, we produced antibodies to bovine beta-casein with controlled specificity, suitable as markers of the integrity of the major bonds involved in its initial breakdown. Sixteen rabbits were immunized with synthetic substitutes for six plasmin-sensitive peptides. Antisera raised to the peptides (f20-39), (f40-56), (f94-113), (f184-202), and (f193-209) recognized beta-casein in ACP-ELISA, Western-blot, and biosensor assays. Casein in vitro hydrolysis by plasmin or chymosin reduced the detection of these determinants in ACP-ELISA, in agreement with the enzymatic sensitivity of bonds included within the binding sites, or in their neighborhood. Antiserum to (f20-39) in particular allowed the specific detection of plasmin cleavage at the bond generating gamma1-CN. Antisera to C-terminus preferentially detected the cleavage by chymosin. Immunoassays using these antibodies would allow in situ monitoring of significant proteolysis events without bias originated in the secondary degradation of the released peptides. 相似文献