落叶松人工林地位指数级导向曲线模型的研究

本文用约1000块样地资抖,系统地研究了黑龙江省落叶松人工林的地位指数级导向曲线模型。通过林业统计分析软件Statistica6.0对各落叶松人工林数据进行拟合、分析比较,结果为：从拟合精度、曲线走向与散点分布趋势、残差分布等综合考虑,舒马切尔曲线函数比较适合落叶松人工林地位指数级导向曲线模型的拟合。     

欧盟共同农业政策面临的挑战及最新改革措施

共同农业政策作为联系欧盟成员国农业的纽带,在经历2003年的改革后,虽然在价格补贴、农村发展等方面取得一定的成效,但仍然面临财政负担、食品安全、新成员国加入等来自内外部的挑战和压力.为应对这些挑战和压力,欧盟进行了降低政策价格、微调配额等一系列的最新改革.     

提高新红星苹果品质的栽培技术

前些年新红星等元帅系短枝型以其丰产易管的优点,受到广大果农的欢迎,大大增加果农的收益,也使许多果农管理粗放,造成新红星口味淡、色泽差。但随着果品总量的增加和人民生活水平的提高,对苹果的质量提出了更高的要求,所以我们采取以下栽培管理技术,取得了较好效果,改善了红星苹果的品质。1、加强土肥水管理由于许多农民片面追求产量,大量偏施氮肥,使果实个头小,风味淡和着色差问题越来越     

Inhibition of K562 cell growth by antisense drug targeting with VEGF mRNA in vitro

AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs.     

Can treatment of <Emphasis Type="Italic">Brachiaria decumbens</Emphasis> (signal grass) improve its utilisation in the diet in small ruminants?—a review

Brachiaria decumbens is an extremely productive tropical grass due to its aggressive growth habit and its adaptation to a varied range of soil types and environments. As a result of the vast availability, treated B. decumbens demonstrates as a promising local material that could be utilised as an improved diet for sheep and goats. Despite the fact that the grass significantly increases weight gains in grazing farm animals, there were many reports of general ill-thrift and sporadic outbreaks of photosensitivity in livestock due to the toxic compound of steroidal saponin found in B. decumbens. Ensiling and haymaking were found to be effective in removing toxin and undesirable compounds in the grass. Biological treatments using urea, activated charcoal, polyethylene glycol, and effective microorganisms were found to be useful in anti-nutritional factor deactivation and improving the nutritive values of feedstuffs. Besides, oral administration of phenobarbitone showed some degree of protection in sheep that fed on B. decumbens pasture. In this review, we aim to determine the effect of B. decumbens toxicity and possible treatment methods on the grass to be used as an improved diet for small ruminant.     

Genomic characterization of the first oral avian papillomavirus in a colony of breeding canaries (<Emphasis Type="Italic">Serinus canaria</Emphasis>)

Papillomaviruses are non-enveloped, DNA viruses that infect skin and mucosa of a wide variety of vertebrates, causing neoplasias or simply persisting asymptomatically. Avian papillomaviruses, with six fully sequenced genomes, are the second most studied group after mammalian papillomaviruses. In this study, we describe the first oral avian papillomavirus, detected in the tongue of a dead Yorkshire canary (Serinus canaria) and in oral swabs of the same bird and other two live canaries from an aviary in Madrid, Spain. Its genome is 8,071 bp and presents the canonical papillomavirus architecture with six early (E6, E7, E1, E9, E2, E4) and two late open reading frames (L1 and L2) and a long control region between L1 and E6. This new avian papillomavirus L1 gene shares a 64% pairwise identity with FcPV1 L1, so it has been classified as a new species (ScPV1) within the Ethapapillomavirus genus. Although the canary died after showing breathing problems, there is no evidence that the papillomavirus caused those symptoms so it could be part of the oral microbiota of the birds. Hence, future investigations are needed to evaluate the clinical relevance of the virus.     

Knowledge,attitudes and practices towards <Emphasis Type="Italic">cystic echinococcosis</Emphasis> in livestock among selected pastoral and agro-pastoral communities in Uganda

A cross-sectional study was done from March 2013 to May 2014 to assess knowledge, attitudes, and practices towards cystic echinococcosis (CE) or hydatidosis among selected pastoral and agro-pastoral communities in Uganda. A structured questionnaire was administered to 381 respondents. Multivariate logistic regression analysis was done to find the relationship between knowledge about CE and factors such as age, sex, and level of education across all regions. The odds ratio and confidence interval were used to determine the difference in responses across regions. It was shown that age above 36 years was significantly (p < 0.001) associated with awareness about CE in livestock. Likewise, uneducated (p < 0.0001) and agro-pastoralists (p = 0.01) were significantly less knowledgeable than the educated and pastoralists across all regions. The overall knowledge towards CE in livestock was low 17.8% (95% CI = 14.0–21.6). Dog ownership was high and they never dewormed their freely roaming dogs. Dogs shared water with livestock. In conclusion, knowledge about CE in livestock was low across all regions. Therefore, public health education and formulation of policies towards its control by the relevant stakeholders should be done. Also, the true prevalence of CE in livestock needs to be done so that the magnitude and its public health significance are elucidated.     

日本北海道北部寒温带森林流域林冠层凋落物及其养分归还量的景观格局(英文)

Within a forested watershed at the Uryu Experimental Forest of Hokkaido University in northern Hokkaido, overstory litterfall and related nutrient fluxes were measured at different landscape zones over two years. The wetland zone covered with Picea glehnii pure stand. The riparian zone was deciduous broad-leaved stand dominated by Alnus hirsuta and Salix spp., while the mixture of deciduous broadleaf and evergreen conifer dominated by Betula platyphylla, Quercus crispula and Abies sachalinensis distributed on the upland zone. Annual litterfall averaged 1444, 5122, and 4123 kg.hm^-2·a^-1 in the wetland, riparian and upland zones, respectively. Litterfall production peaked in September-October, and foliage litter contributed the greatest amount （73.4%-87.6 %） of the annual total litterfall. Concentrations of nutrients analyzed in foliage litter of the dominant species showed a similar seasonal variation over the year except for N in P glehnii and A. hirsuta. The nutrient fluxes for all elements analyzed were greatest on riparian zone and lowest in wetland zone. Nutrient fluxes via litterfall followed the decreasing sequence： N （11-129 kg.hm-2.aq） 〉 Ca （9-69） 〉 K （5-20） 〉 Mg （3-15） 〉 P （0.4-4.7） for all stands. Significant differences were found in litterfall production and nutrient fluxes among the different landscape components. There existed significant differences in soil chemistry between the different landscape zones. The consistently low soil C：N ratios at the riparian zone might be due to the higher-quality litter inputs （largely N-fixing alder）.     

Concentration variations of several ions in stream after a wildfire

In May 2006, a high intensity wildfire occurred in Songling forest region in Daxing＇an Mountains, China. The concentration changes of eight ions （K^＋, Na^＋, Ca^2＋, Mg^2＋, Cl^-, Br^-, NO3^- and SO4^2-） were measured in burned and tmbumed streams after fire from May to Oct., 2006. Results show that the most ions flux were higher in burned stream than that in unburned stream during the sampling period, and the greatest concentrations of most ions transported from burned stream occurred in July. After fire, the most amplitude chemical ion was Ca^2＋, whose average concentration was 5.50 mg·L^-1 higher than that in unburned stream, and the total concentration of every chemical ion presents a trend Ca^2＋〉SO4^2- 〉Na^＋〉Mg^2＋〉NO3^- . The average concentrations of Ca^2＋, SO4^2- , Na^＋＇Mg^2＋,NO3^- showed an increase trend, but those of K^＋, Cl^- , Br had a decreased trend. SO4^2- had the largest loss among these anions, followed by NO3^-. Overall, the increase degree of cation was greater than that of anion after burning.     

BCG诱导RAW264.7细胞脂肪酸氧化对细胞自噬和促炎因子表达的调控作用

旨在探究脂肪酸氧化（fatty acid oxidation,FAO）对BCG介导的RAW264.7细胞自噬和促炎因子表达的调控作用。用BODIPY染色和游离脂肪酸定量试剂盒检测BCG感染后RAW264.7细胞中脂滴聚集情况以及脂肪酸含量;Western blot检测BCG感染对肉毒碱棕榈酰基转移酶1A （CPT-1A）表达的影响;Etomoxir （100 μmol·L^-1）预处理细胞2 h后,BCG感染细胞6 h,检测RAW264.7细胞中BCG存留量,并用Western blot方法检测自噬相关蛋白（Beclin1、LC3-II）和溶酶体蛋白（Rab7）的表达情况;用免疫荧光方法和mRFP-GFP-LC3荧光双标腺病毒分别检测自噬小体聚集和自噬流;荧光定量PCR和ELISA分别检测促炎因子IL-1β、IL-6和TNF-α mRNA表达情况以及在细胞培养上清中的含量。结果显示,BCG感染促进RAW264.7细胞中脂滴聚集和CPT-1A的表达,而游离脂肪酸含量降低;Etomoxir预处理抑制了细胞中BCG存活,并上调了Beclin1、LC3-II和Rab7表达,且细胞中出现大量自噬小体聚集,自噬流增强,却抑制了促炎因子IL-1β、IL-6和TNF-α mRNA表达与分泌。综上表明,抑制FAO可促进BCG感染诱导的RAW264.7细胞自噬,并抑制BCG感染引起的炎症反应。