濒危植物羽叶丁香组织培养

以濒危植物羽叶丁香的芽和种子为外植体,采用植物组织培养法,研究了不同消毒方法对无菌体系建立、不同浓度的蔗糖和激素组合对种子初代培养、不同基本培养基和激素组合对增殖和生根的影响,以期为羽叶丁香的组培快繁、种质资源保护和开发利用提供依据。结果表明:新枝为较适宜的外植体,适宜的消毒方式为75%酒精消毒30s,0.1%升汞消毒7min,最适增殖培养基为MS+5.0mg·L~(-1) 6-BA+0.01mg·L~(-1) IBA;最适种子初代培养基为MS+20g·L~(-1)蔗糖汁+3.0mg·L~(-1) 6-BA+0.10mg·L~(-1) IBA,最适增殖培养基为MS+7.0mg·L~(-1) 6-BA+0.05mg·L~(-1) IBA;最适生根培养基为WPM+2.0mg·L~(-1) IBA。     

Assessment of gut microbiota in different developmental stages of Malaysian Mahseer (Tor tambroides)

Malaysian Mahseer (Tor tambroides) has a good prospect for aquaculture because of its high market demand. However, there is a scarce information on gut microbiota associated with Malaysian Mahseer unlike other fish species. Therefore, we constructed and compared gut microbiota in different developmental stages (larval, juvenile, fingerling, yearling, and adult) using culture dependent and PCR‐DGGE fingerprinting technique for better understanding of gut microbiota composition associated with T. tambroides. Culturable gut microbiota composition in all developmental stages were composed of β‐ and γ‐Proteobacteria, and Bacilli. Biodiversity analysis of culturable gut microbiota showed that larval, juvenile, and adult stages have higher diversity than fingerling and yearling stages. Ward's linkage cluster analysis showed that culturable gut microbiota composition in larval and juvenile stages were close to adult stages, whereas fingerling and yearling stage composed same cluster. PCR‐DGGE fingerprinting technique showed that unculturable gut microbiota were constituted by α‐and γ‐Proteobacteria, and Actinobacteria. Ward's linkage cluster analysis showed that unculturable gut microbiota composition in both larval and juvenile stages were distinct from other developmental stages. Our results revealed that gut microbiota composition were varied in different developmental stages of Malaysian Mahseer and continuous shifts of gut microbiota from larval to adult stages.     

运用土壤水盐运移模型优化棉花微咸水膜下滴灌制度

为优化设计棉花微咸水膜下滴灌灌溉制度,利用HYDRUS建立含根系吸水项的土壤水盐运移数值模拟模型,并用田间试验资料进行参数识别和模型验证。用验证认可的数值模型优化设计棉花微咸水膜下滴灌和非生育期洗盐灌溉制度,并预测棉花生育期水盐运移规律和长期效应。结果表明:模型仿真度较高、运行速度较快;T检验说明土壤含水率和电导率模拟值与实测值均无显著差异;模型参数中形状系数α、n对含水率影响较大,纵向弥散度DL对电导率影响较大;当地膜下滴灌适合生育期一膜双管、低额高频灌溉,并结合非生育期1年1次或2年1次大水漫灌洗盐,20a的模拟结果显示此灌溉制度下不会引起土壤次生盐渍化。     

华榛、羽叶丁香种子形态构造特点的研究

对两种珍稀濒危植物华榛（Ｃｏｒｙｉｕｓ ｃｈｉｎｅｎｓｉｓ Ｆｒａｎｃｈ）和忌叶丁香（Ｓｙｒｉｎｇａ ｐｉｎｎａｔｉｆｉａ Ｈｅｍｅｌ）种子的形态结构进行了研究;就其种子的形态结构特点与植物濒危的关系进行了初步探讨。     

珍稀濒危植物──蝟实果实形态构造特点研究

对国家三级保护植物实（ＫｏｌｋｗｉｔｚｉａａｍａｂｉｌｉｓＣｒａｅｂｎ）果实的形态结构特点进行了研究，并就其种子形态结构特点与植物珍稀濒危的关系进行了初步探讨。     

Evaluation of mulched drip irrigation for cotton in arid Northwest China

Field experiments were conducted in arid Southern Xinjiang, Northwest China, for 3 years to evaluate sustainable irrigation regimes for cotton. The experiments involved mulched drip irrigation during the growing season and flood irrigation afterward. The drip irrigation experiments included control experiments, experiments with deficit irrigation during one crop growth stage, and alternative irrigation schemes in which freshwater was used during one growth stage and relatively saline water in the others. The average cotton yield over 3 years varied between 3,575 and 5,095 kg/ha, and the irrigation water productivity between 0.91 and 1.16 kg/m³. Crop sensitivities to water stress during the different growth stages ranged from early flowering-belling (most sensitive) > seedling > budding > late flowering-belling (least sensitive), while sensitivities to salt stress ranged from late flowering-belling > budding > seedling > early flowering-belling. Although mulched drip irrigation during the growing season caused an increase in salinity in the root zone, flood irrigation after harvesting leached the accumulated salts to below background levels. Numerical simulations, based on the 3-year experiments and extended by another 20 years, suggest that mulched drip irrigation using alternatively fresh and brackish water during the growing season and flood irrigation with freshwater after harvesting is a sustainable irrigation practice that should not lead to soil salinization.     

双断根嫁接对番茄生长及果实产量、品质的影响

以野原2号为砧木,春棚1号为接穗,当砧木5片真叶期、接穗4片真叶期时,采用双断根嫁接方法进行嫁接,探究双断根嫁接对番茄生长及果实产量、品质的影响。试验结果表明,嫁接可以显著提高番茄植株长势和产量,对果实中VC、可溶性糖和可溶性蛋白质含量影响不大。与常规砧木未断根的嫁接相比,双断根嫁接显著提高番茄植株生长后期(坐果期、采收期)的株高及茎粗,显著提高了产量;2种嫁接方法的果实横径、纵径及单果质量均差异不显著。     

不同生育时期干旱胁迫对马铃薯生理生化指标的影响

研究不同生育时期干旱胁迫下马铃薯相关生理生化指标的变化,以期探讨干旱胁迫下马铃薯抗旱的生理机制。选取生产上推广的‘冀张薯8号’和‘夏波蒂’两个马铃薯品种,在旱棚内分区栽培。不同生育时期进行干旱处理,测定马铃薯超氧化物歧化酶（SOD）、丙二醛（MDA）、脯氨酸（Pro）的含量变化。结果表明,各生育时期干旱胁迫下,马铃薯MDA、Pro含量均增加,而SOD活性下降。花期干旱胁迫对马铃薯的生理生化指标影响最大;抗性强的品种MDA、脯氨酸含量增加的幅度较小,SOD的活力较高,而抗旱性弱的品种则相反。抗旱性强的品种在生理生化指标上具有抗旱的调节机制。     

Photosynthesis,growth and yield of soybean and maize in a tree-based agroforestry intercropping system on the Loess Plateau

Agroforestry is the most effective way to restore the disturbed lands on the Loess Plateau and to develop the poor local economy. In order to maximize the potential benefits of tree-based intercropping systems, photosynthesis, growth and yield of soybean and corn were studied by measuring photosynthetic active radiation (PAR), plant water deficit and soil moisture in a 4-year-old plantation of walnut (Juglans regia L.) and plum (Prunus salicina) grown at a spacing of 5 m × 3 m on the Loess Plateau. The effects of tree competition significantly reduced PAR, net assimilation (NA), growth and yield of individual soybean or corn plants growing nearer (1 m near tree row) to tree rows. NA was highly correlated with growth and yield of the both crops. These correlations were higher for corn than soybeans, with corn, rather than soybeans being more adversely impacted by tree shading. Plum, rather than walnut had the greatest competitive effect on PAR and NA. Daily plant water deficits were non-significantly and poorly correlated with NA and growth and yield of the both crops. However, soil moisture (20 cm depth) was significantly correlated with biomass and yield of both crops. Possible remediation strategies are discussed to reduce tree competitive interactions on agricultural crops.     

粉尘污染对侧柏叶部分生理指标的影响

对受污染后黄帝陵与药王山两地侧柏叶的一些生理指标的测定结果表明,水泥粉尘污染后侧柏叶的光合强度,呼吸强度和蒸腾强度均有不同程度的降低;气孔扩散阻力增大,方差分析显示,两地间污染程度差异极显著,药王山侧柏整体污染程度重于黄帝陵。现存污染条件下,黄帝陵侧柏不会死亡,但生长将受到影响,同时也严重影响黄帝陵的自然景观