全文获取类型
收费全文 | 296篇 |
免费 | 9篇 |
国内免费 | 1篇 |
专业分类
林业 | 21篇 |
农学 | 14篇 |
基础科学 | 1篇 |
58篇 | |
综合类 | 12篇 |
农作物 | 12篇 |
水产渔业 | 13篇 |
畜牧兽医 | 153篇 |
园艺 | 5篇 |
植物保护 | 17篇 |
出版年
2023年 | 3篇 |
2022年 | 8篇 |
2021年 | 16篇 |
2020年 | 8篇 |
2019年 | 6篇 |
2018年 | 5篇 |
2017年 | 6篇 |
2016年 | 9篇 |
2015年 | 11篇 |
2014年 | 20篇 |
2013年 | 32篇 |
2012年 | 16篇 |
2011年 | 30篇 |
2010年 | 13篇 |
2009年 | 15篇 |
2008年 | 23篇 |
2007年 | 17篇 |
2006年 | 18篇 |
2005年 | 9篇 |
2004年 | 9篇 |
2003年 | 15篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 1篇 |
1995年 | 1篇 |
1992年 | 2篇 |
1987年 | 1篇 |
1984年 | 1篇 |
1978年 | 1篇 |
排序方式: 共有306条查询结果,搜索用时 15 毫秒
1.
Seasonal variation in pigmentation and anthocyanidin phenetics in commercial Eustoma flowers 总被引:2,自引:0,他引:2
A. F. M. Jamal Uddin Fumio Hashimoto Toshiki Miwa Katsuhiro Ohbo Yusuke Sakata 《Scientia Horticulturae》2004,100(1-4):103-115
The seasonal change in petal color and pigmentation of 29 commercial Eustoma cultivars was studied. The flowers are basically divided into four groups according to the major anthocyanidin phenotype in association with petal coloration, i.e., delphinidin (Dp)-based (purple flower), cyanidin (Cy)-based (reddish purple flower), pelargonidin (Pg)-based (pink flower), and none (white flower) groups. The constitution of petal anthocyanidins was not changed by forcing treatment in most of the flowers. Lightness (L*) and chroma (C*, color saturation) showed a change along with the increase/decrease of hue angle difference (ΔH*), thus simultaneously the chromatic tonalities tended to move to redder and bluer, respectively. Floral pigment clustering described two flower groups in a dendrogram, based on anthocyanidin constitutions as phenetic markers, which are apparently the Dp- and Pg-based phenotypes of anthocyanidin syntheses. The Cy-based flowers made a subcluster with the Pg-based flowers, indicating a close relationship in the biosynthesis of the two anthocyanidins, and suggesting the Dp- and Pg-syntheses complement one another. 相似文献
2.
Satoshi Furukawa Naho Tsuji Seigo Hayashi Yusuke Kuroda Masayuki Kimura Chisato Hayakawa Kazuya Takeuchi Akihiko Sugiyama 《Journal of toxicologic pathology》2022,35(1):37
We investigated the morphological effects of testosterone on placental development in a rat model of polycystic ovarian syndrome (PCOS). Testosterone propionate (TP), which was subcutaneously administered to pregnant rats with 5 mg/animal from gestation day (GD) 14 to GD 18, induced a maternal weight reduction without mortality or clinical signs from GD 19 onwards. A decrease in fetal and placental weight, an increase in intrauterine growth retardation (IUGR) rates, and histological changes in the placenta were observed on GD 21 but not on GD15 or 17. Histopathologically, on GD 21, the trophoblast septa thickened, and the maternal sinusoids were narrowed in the labyrinth zone, resulting in a small placenta. Additionally, the placental weight, thickness, and histological morphology in the labyrinth zone on GD 21 in the TP-treated group were nearly identical to those on GD 17 in the control and TP-treated groups. Therefore, it was assumed that the testosterone-induced small placenta was induced in association with the developmental inhibition of the fetal part of the placentas from GD 17 onwards. 相似文献
3.
Takashima H Sakai H Yanai T Masegi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(2):171-174
To detect serum antibody against Pasteurella multocida (P. multocida) in infected rabbits. a modified immunoperoxidase assay was applied. An outbreak of P. multocida infection in rabbits started from sudden death. The infected rabbits had severe fibrinous and purulent pneumonia with hemorrhage, and a large number of P. multocida (A:12) was isolated from the trachea and lungs of the animals. Antibodies of IgM and IgG to P. multocida were assessed by immunohistochemical staining using the sera of the animals as primary antibodies and applying them to formalin-fixed, paraffin-embedded sections of P. multocida attached to calf fibrin. IgM antibodies to P. multocida were first detected 7 days after the onset of the disease. IgG antibodies began to rise on the 7th or 14th day. These results suggested that the modified immunoperoxidase assay could detect antibodies against P. multocida. 相似文献
4.
Katsuji UETAKE Toshie ISHIWATA Naoshige ABE Yusuke EGUCHI Toshio TANAKA 《Animal Science Journal》2005,76(6):581-586
The differential variables for carcass grades were identified from morphological, behavioral and physiological measurements of young steers at a commercial farm. Thirty‐five Japanese Black × Holstein steers aged 6–10 months were randomly assigned to three pens. The steers had free access to ryegrass hay and a restricted amount of concentrate diet for the first 6 months of trial. They were then given oat straw ad libitum and an additional concentrate diet until slaughter. Behavioral observations (15 categories) were carried out once every 2 weeks for 2 h after feeding. Bodyweight was measured, blood sampled (for seven metabolic hormones and five metabolites), ultrasonic scanning conducted and physical measurements taken (10 parts for each animal) in months 1, 3 and 5 (early fattening stage, EFS) and in months 7, 9 and 11 (middle fattening stage, MFS) of the trial. Temperament scores during these procedures, entry order into restraint stalls, social rank and average daily gain were also assessed. A factor analysis and one‐way anova were used to identify clusters of variables which had different factor scores between carcass grades. As for the carcass yields, a cluster of chest girth and depth, bodyweight, withers and hip height, and rump length (P < 0.01), and a cluster of the frequency of scratching the body with facilities (P < 0.05) were identified as differential variables in the EFS. In the MFS, a cluster of concentrations of cortisol and epinephrine, and the frequency of eating straw were identified (P < 0.10). As for the carcass quality grade, a cluster of social rank, triglyceride concentration and the frequencies of stand‐chewing cud and eating hay, and a cluster of the frequency of investigating facilities (both P < 0.10) were identified in the EFS. In the MFS, a cluster of hip height, bodyweight, cannon circumference, chest depth, withers height and chest girth, and a cluster of the frequencies of eating a concentrate diet and stand‐chewing the cud, and social rank (both P < 0.10) were identified. In conclusion, bodyweight, chest girth and depth, withers and hip height were identified as good differential variables for future carcass grades of young steers. Facility enrichment that encourages steers to eat hay in the EFS, and to eat a concentrate diet in and after the MFS would be effective in upgrading carcass grades. High incidence of investigating and scratching the body with facilities and stand‐chewing the cud might lead to lower carcass grades. 相似文献
5.
Okabayashi K Kano R Sirouzu K Yanai T Mizuno M Yamamura H Saegusa S Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(8):953-955
Two feline cases were diagnosed as systemic cryptococcosis due to Cryptococcus neoformans (teleomorph: Filobasidiella neoformans) by PCR assay with CAP59 gene primers using urine, serum and biopsy samples. The results of molecular analysis were consistent with the mycological findings. 相似文献
6.
Suzuki M Uchida K Morozumi M Hasegawa T Yanai T Nakayama H Tateyama S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(11):1233-1239
Canine necrotizing meningoencephalitis (NME) and granulomatous meningoencephalomyelitis (GME) were compared pathologically. Gross observation exhibited lateral ventricular dilation and discoloration, malacia and/or cavitation of the cerebrum in NME. On the contrary, gross changes were milder in GME, except for occasional visible granulomatous mass formation. Histopathologically, the lesions of NME were distributed predominantly in the cerebral cortex and various degrees of inflammatory and necrotic changes were observed according to clinical stages. Besides, microscopic lesions of GME were mainly distributed in the white matter of the cerebrum, cerebellum and brainstem, which are characterized by perivascular cuffing, multiple granulomas and leptomeningeal infiltrates. Although macrophages and lymphocytes were predominant in the inflammatory lesions of both disorders, macrophages in GME transformed into epithelioid cells and exhibited more massive infiltration. Although lectin RCA-1-reactive cells were numerous in both disorders, lysozyme immunoreactive cells in NME were fewer than that in GME. Parenchymal infiltration of MAC387-positive cells was common in GME and limited in NME. The number of CD3-positive lymphocytes in the GME lesions tended to be greater than in NME, though the difference was not statistically significant. Morphological and immunohistochemical differences of the lesions, in particular, the characteristics of infiltrative macrophages may reflect these different pathogeneses of the two disorders. 相似文献
7.
Sakai H Nakano H Yamaguchi R Yonemaru K Yanai T Masegi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):731-735
A new canine cell line, named CCT, was established from the cutaneous malignant histiocytosis in a 4-year-old male Borzoi. CCT proliferated with loose adherence and doubling time was approximately 30 hr. When co-cultured with latex beads, CCT phagocytized beads vigorously. Lysozyme and vimentin were positive by immunostaining, and non-specific esterase and acid phosphatase were positive by cytochemical staining. These features indicated the cells had a histiocytic nature. Furthermore, by subcutaneous injection to nude mice CCT could successfully form tumors with the morphological and immunohistochemical features similar to the original tumor. 相似文献
8.
Sawada Y Yanai T Nakagawa H Tsukamoto Y Tamagawa Y Yokoi S Yanagi M Toya T Sugizaki H Kato Y Shirakura H Watanabe T Yajima Y Kodama S Masui A 《Pest management science》2003,59(1):49-57
Nineteen analogues were synthesized by modifying the tert-butylhydrazine moieties of N'-tert-butyl-N'-(3,5-dimethylbenzoyl)-5-methyl-2,3-dihydro-1,4-benzodioxine-6-carbohydrazide and N'-tert-butyl-N'-(3,5-dimethylbenzoyl)-5-methylchromane-6-carbohydrazide (chromafenozide), and the synthesized analogues were evaluated for their insecticidal activity against Spodoptera litura F. While all of the synthesized analogues had insecticidal activity inferior to those of the lead compounds, several of the analogues nonetheless showed high insecticidal activity. Chromafenozide has shown very high selectivity toward lepidopteran species. 相似文献
9.
Tadahisa MASHITA Hiroaki KAMISHINA Yuya NAKAMOTO Yosuke AKAGI Ataru NAKANISHI Yusuke HARASAKI Tsuyoshi OZAWA Takashi UEMURA Yui KOBATAKE Shunsuke SHIMAMURA Naoki KITAMURA Sadatoshi MAEDA Yuji UZUKA Gerry SHAW Jun YASUDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):433-438
The aim of this study was to evaluate the prognostic value of concurrent measurement of
serum phosphorylated neurofilament heavy subunit (pNF-H) concentration and intramedullary
T2W hyperintensity in paraplegic to paraplegic dogs. Our hypothesis was that concurrent
measurement of these would provide a more accurate prediction of functional outcome in
dogs with thoracolumbar intervertebral disc herniation (IVDH). A prospective case-control
clinical study was designed using 94 dogs with acute onset of thoracolumbar IVDH. The
association of serum pNF-H concentration, T2W hyperintensity on sagittal MRI (T2H/L2),
deep pain perception and surgical outcome were evaluated with logistic regression analysis
after three months for all 94 surgically treated dogs. Sensitivity to predict
non-ambulatory outcome was compared among pNF-H and T2H/L2 and combination of both.
Logistic regression analysis indicated that serum pNF-H concentration and T2H/L2 were
significantly correlated with surgical outcome (P<0.05); however, deep
pain perception was not (P=0.41). The results of logistic regression
analysis indicated that the odds ratios of unsuccessful long-term outcome were 2.6 for
serum pNF-H concentration, 1.9 for T2H/L2 and 2.3 for deep pain sensation. The sensitivity
and specificity to predict non-ambulatory outcome for using serum parameter pNF-H>2.6
ng/ml, using T2H/L2 value of>0.84 and using both
serum pNF-H and T2H/L2, were 95% and 75.7%, 65% and 86.5%, and 90.0% and 97.5%,
respectively. Therefore, combined measurements of serum pNF-H and T2H/L2 might be useful
for predicting long-term outcome in dogs with thoracolumbar IVDH. 相似文献