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排序方式: 共有93条查询结果,搜索用时 15 毫秒
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Kingston JK Bayly WM Sellon DC Meyers KM Wardrop KJ 《American journal of veterinary research》2002,63(6):840-844
OBJECTIVE: To investigate the effects of formaldehyde fixation on equine platelets using flow cytometric methods to evaluate markers of platelet activation. SAMPLE POPULATION: Blood samples from 6 Thoroughbreds. PROCEDURE: The degree of fluorescence associated with binding of fluorescein isothiocyanate (FITC)-conjugated anti-human fibrinogen antibody and FITC-annexin V in unactivated and adenosine diphosphate (ADP)-, platelet activating factor (PAF)-, and A23187-activated platelet samples in unfixed and 0.5, 1.0, and 2.0% formaldehyde-fixed samples was assessed by use of flow cytometry. RESULTS: In samples incubated with FITC-anti-human fibrinogen antibody prior to fixation, addition of 2.0% formaldehyde resulted in a 30% increase in total fluorescence in ADP- and PAF-activated samples and a 60% increase in A23187-activated samples. Fixation for 24 hours prior to addition of antibody resulted in reduced fluorescence of samples containing antihuman fibrinogen antibody for all 3 concentrations of formaldehyde in PAF-activated samples. The addition of all 3 concentrations of formaldehyde after incubation with FITC-annexin V resulted in significant increases in fluorescence in unactivated and activated platelet samples. As length of fixation time increased, there was a gradual increase in fluorescence that was significant at 24 hours. CONCLUSIONS: Because fixation with 2.0% formaldehyde results in significant changes in fluorescence in activated platelet samples containing anti-fibrinogen antibody, lower concentrations of formaldehyde should be used to fix equine platelet samples. Formaldehyde-fixed platelet samples should be analyzed within 12 hours of fixation to avoid artifactual increases in fluorescence. Fixation of samples containing FITC-annexin V should be avoided because of significant increases in fluorescence that may interfere with interpretation of results. 相似文献
3.
Warwick JW Evans DR Romig JH Alexander JK Desch MD Kaiser ML Aubier M Leblanc Y Lecacheux A Pedersen BM 《Science (New York, N.Y.)》1982,215(4532):582-587
Planetary radio astronomy measurements obtained by Voyager 2 near Saturn have added further evidence that Saturnian kilometric radiation is emitted by a strong dayside source at auroral latitudes in the northern hemisphere and by a weaker source at complementary latitudes in the southern hemisphere. These emissions are variable because of Saturn's rotation and, on longer time scales, probably because of influences of the solar wind and Dione. The electrostatic discharge bursts first discovered by Voyager 1 and attributed to emissions from the B ring were again observed with the same broadband spectral properties and an episodic recurrence period of about 10 hours, but their occurrence frequency was only about 30 percent of that detected by Voyager 1. While crossing the ring plane at a distance of 2.88 Saturn radii, the spacecraft detected an intense noise event extending to above 1 megahertz and lasting about 150 seconds. The event is interpreted to be a consequence of the impact, vaporization, and ionization of charged, micrometer-size G ring particles distributed over a vertical thickness of about 1500 kilometers. 相似文献
4.
Warwick JW 《Science (New York, N.Y.)》1963,140(3568):814-816
5.
Warwick JW 《Science (New York, N.Y.)》1960,132(3435):1250-1252
Since the spring of 1960 a strong positive correlation between Jupiter's decametric emission and solar decametric continuum emission observed at Boulder has been evident. The time delay of 1 to 2 days, with solar emission preceding Jupiter's emission, suggests that fast solar corpuscles, at velocities of the order of 0.1 c, are directly involved in the planet's atmosphere or magnetic field. 相似文献
6.
The measurement sensitivity of the pointing direction of a laser beam is ultimately limited by the quantum nature of light. To reduce this limit, we have experimentally produced a quantum laser pointer, a beam of light whose direction is measured with a precision greater than that possible for a usual laser beam. The laser pointer is generated by combining three different beams in three orthogonal transverse modes, two of them in a squeezed-vacuum state and one in an intense coherent field. The result provides a demonstration of multichannel spatial squeezing, along with its application to the improvement of beam positioning sensitivity and, more generally, to imaging. 相似文献
7.
The effects of animal treading on denitrification in a mixed ryegrass-clover pasture were studied. A single treading event of moderate or severe intensity was applied in plots during spring by using dairy cows at varying stocking rates (4.5 cows 100 m−2 for 1.5 or 2.5 h, respectively). Treading caused a significant short-term 21 days) increase in denitrification. Denitrification rates reached a maximum of 52 g N2O-N ha−1 day−1 at 8 days after severe treading compared to 2.3 g N2O-N ha−1 day−1 under nil treading. Thereafter, denitrification rates declined, and were similar to non-trodden control plots after 28 days. Soil aeration, was significantly reduced by treading as expressed by water-filled porosity. In addition, soil NH4+-N and NO3−-N concentrations were also increased by treading. We propose that the underlying processes involved in increasing denitrification under treading were two-fold. Firstly, treading caused a temporary (e.g. 3 days after treading) reduction in soil aeration through soil physical damage, and secondly, reduced soil N utilisation prompted by reduced plant growth led to increased soil NH4+-N and NO3−-N availability. This study shows that treading, without the influence of other grazing animal factors (e.g. excretion), can cause a large short-term stimulation of denitrification in grass-clover pastures. 相似文献
8.
S. I. Warwick R. K. Gugel T. McDonald K. C. Falk 《Genetic Resources and Crop Evolution》2006,53(2):297-312
Information on genetic diversity and genetic relationships among genotypes of Brassica carinata is currently limited. The objectives of this study were to evaluate patterns and levels of genetic diversity in B. carinata based on amplified fragment length polymorphisms (AFLP) as compared with Brassica juncea and Brassica nigra, and to evaluate agronomic and seed quality data for plants grown in the field in western Canada. A total of 296 AFLP bands
were generated from four primer pair combinations and scored for presence/absence in 66, 20 and 7 accessions of B. carinata, B. juncea and B. nigra, respectively. B. carinata was less genetically diverse than the other two species. Differences in diversity were evident in the proportion of polymorphic
loci within each species: 23, 35 and 50% for B. carinata, B. nigra and B. juncea, respectively. Pair-wise similarity measures based on the Jaccard coefficient were highest among accessions of B. carinata and showed the narrowest range: 0.911 (0.810–0.981) compared to B. nigra: 0.569 (0.438–0.660) and B. juncea: 0.715 (0.345–0.951). AFLP-based genetic distance information can be used by plant breeders to select diverse genotypes.
AFLPs are also useful for fingerprinting cultivars and two primer pair combinations were sufficient to uniquely identify all
the accessions of B. carinata. More variation among accessions was identified in the agronomic trial than had previously been described in studies of B. carinata in western Canada, but the data were too limited to draw conclusions regarding specific accessions. Overall, the findings
were in agreement with other published work describing the favourable agronomic potential of this species. 相似文献
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