Quantification of Inorganic Anions in Tea (Camellia Sinensis (L) O. Kuntze) Tissues and Soil Using Ion Chromatography Coupled with Conductivity Detector

A simple ion chromatographic method is proposed for the simultaneous quantification of five inorganic anions in samples derived from tea system with a suppressed conductivity detector. For this purpose extracts of leaves, roots and soils of tea obtained at room temperature yielded chromatographic profiles with substantial differences in the relative contents of fluoride (F^?), chloride (Cl^?), nitrate (NO₃^?) and sulfate (SO₄ ^2-). Although Cl^? and SO₄^2- were common to each extracted sample in different categories and accounted for the majority of the anions present, one additional anion peak was detected in root and leaf tissues. Irrespective of the clone and sample investigated the average F^? content varied from 253 to 1664 mg kg^?1, Cl^? varied between 60 and 1789 mg kg^?1, NO₃^? varied from 119 to 188 mg kg^?1 and SO₄^2- varied between 55 and 6779 mg kg^?1. The anionic load was at its crest in clone UPASI – 3 in all the three categories of samples with few exceptions. Among the samples investigated, tea roots contained a considerable amount of major anions, while tea soils had the lowest amount of the detected anions. The recovery of all the quantified anions was above 95%. The correlation coefficient for all anions where above 99.50%. The concentration of nitrite (NO₂^?) was below the limits of detection based on the analytical technique adopted. Anion nitrate was not detected in tea root and tea leaf extracts.     

Development of a transformation protocol for Tecomella undulata (Smith) Seem from cotyledonary node explants

A protocol was optimized for genetic transformation of Rohida (Tecomella undulata) from cotyledonary node tissues using Agrobacterium tumefaciens strain GV2260 harboring binary vector pBinAR containing osmotin and nptII gene under control of CaMV35S promoter. This is the first report on the transformation of T. undulata. The effective concentration of selectable marker antibiotic used for screening of transformants was 85.97 μM in shooting media and 42.98 μM in rooting media. PCR and Southern blot confirmed integration of osmotin gene into genome of Rohida.     

Utilization of marine fisheries wastes for the production of the freshwater fish Cyprinus carpio

Tropical Animal Health and Production - The present study was aimed to assess the effects of complete replacement of fish meal with fisheries waste meals on survival, growth performance, digestive...     

Complement test for distinctiveness,uniformity and stability testing of kharif onion (Allium cepa L.) varieties

Genetic Resources and Crop Evolution - The distinctiveness, uniformity and stability (DUS) testing is important for varietal identification and protection of plant variety. It has become one of the...