Single and Multistrain Rhizobial Inocula for Pinto and Black Bean Cultivars

ABSTRACT

Common bean (Phaseolus vulgaris L.) is relatively poor in dinitrogen (N₂) fixation, so selecting compatible host cultivar and Rhizobium strain combinations may offer an improvement. The effectiveness of six rhizobial strains was evaluated using five bean cultivars of bean (three pinto and two black bean) in a growth-room experiment. We subsequently selected the three best strains to assess whether multi-strain inoculation had advantages over single-strain inoculation in growth-room and field experiments. In the first-growth-room experiment, Rhizobium strains UMR 1899, RCR 3618, and USDA 2676 were selected for high nodulation, plant dry weight, shoot nitrogen (N), and N₂ fixation. In a second growth-room experiment, the individual strains and a mixture of the three strains generally did not differ in the parameters evaluated. Total shoot N accumulated ranged from 172.9 to 162.8 mg plant^?1, of which 32.1% to 33.6% (equivalent to 54.0 to 59.2 mg plant^{? 1}) was fixed. In field experiments, plant biomass and seed N₂ fixed did not differ among the inoculants at any site. These results suggest that the three strains were equally effective and that the multi-strain inoculant offered no consistent advantage over the single-strain inoculants.     

Response of Determinate and Indeterminate Common Bean Genotypes to Rhizobium Inoculant in a Short Season Rainfed Production System in the Canadian Prairie

ABSTRACT

Common bean (Phaseolus vulgaris L.) has been shown to be a poor di-nitrogen (N₂) fixer and nitrogen (N) fertilizers are usually recommended in bean production. Recent research results suggest that the success of the bean/Rhizobium symbiosis may depend, in part, on the specific bean genotype. Twelve dry bean genotypes differing in growth habit, commercial class, and maturity were evaluated for N₂ fixation in field experiments. Response to inoculant application was highly influenced by environmental conditions. Genotypes differed in nodule dry mass, seed yield, seed N yield, and in amount of N₂ fixed. Growth habit alone was not adequate in classifying bean genotypes for N₂ fixation. The actual amount of N₂ fixed was low ranging from 16 kg ha^?1 to 27 kg ha^?1, suggesting that the symbiotic process alone may not provide adequate N for optimum seed yield in dry environments.