Acquisition capability of the grapevine Flavescence dorée by the leafhopper vector Scaphoideus titanus Ball correlates with phytoplasma titre in the source plant

Flavescence dorée (FD) is one of the most economically important grapevine diseases in Southern Europe, and it is associated with phytoplasmas, phloem-limited wall-less bacteria. Recovery from disease naturally occurs in infected grapevines during the following seasons after infection. The capability of the leafhopper vector Scaphoideus titanus to acquire FD phytoplasma (FDP) from recovered and infected grapevines of Barbera and Nebbiolo varieties was investigated in North-western Italy vineyards monitored from 2007 to 2011. Pathogen concentration was quantified by real-time PCR in FDP-infected grapevines and broad beans, also used as source plants under controlled conditions, to correlate acquisition capabilities and phytoplasma titre in source plants. S. titanus acquired FDP from infected, but not from recovered, grapevines. FDP titre was higher in Barbera than in Nebbiolo and higher in summer than in spring, and acquisition efficiency and pathogen titre in source plants were positively correlated, both in field and laboratory conditions. Recovered plants do not represent a source of inoculum for the vector and therefore do not contribute to FDP spread. The inability of recovered plants to serve as FDP acquisition sources for the vector as well as the effect of the season and of the two grapevine varieties on the FDP acquisition efficiency are relevant results to re-design disease management practices, especially since insecticide treatments against the vector are not fully effective, and newly designed successful control strategies are required.     

Molecular detection of Theileria spp. in sheep and vector ticks in the North Khorasan Province, Iran

A survey was carried in North Khorasan Province, Iran in 2010–2011, designed to identify Theileria spp. infections of both sheep and ticks. The tick species were also examined. Ninety sheep from different flocks were clinically examined, and blood samples and ixodid ticks were collected. Light microscopy of blood smears revealed Theileria spp. infection in 37 (41.1 %), while 74 (82.2 %) of blood samples were positive using semi-nested PCR. Theileria ovis, Theileria lestoquardi, and mixed infection were detected in 63/90 (70 %), 5/90 (5.5 %), and 6/90 (6.6 %) of samples, respectively. Of the 434 ticks that were collected, the most prevalent species was Rhipicephalus turanicus (69.3 %) followed by Hyalomma marginatum turanicum (18.4 %), Dermacentor marginatus (6.4 %), and Rhipicephalus bursa (5.7 %). The ticks were separated into 42 tick pools, and the salivary glands were dissected out in 0.85 % (w/v) saline under a stereomicroscope and examined using semi-nested PCR. Three pools of H. marginatum turanicum salivary glands were infected with T. ovis and T. lestoquardi, and one pool of R. turanicus was infected with T. ovis. Based on these results, it is concluded that the prevalence of T. ovis is higher than T. lestoquardi and that H. marginatum turanicum and R. turanicus are likely vectors of T. lestoquardi and T. ovis in this area.     

Effects of feeding roasted safflower seeds (variety IL-111) and fish oil on dry matter intake, performance and milk fatty acid profiles in dairy cattle

Safflower seed has the highest concentration of linoleic acid among 80 oilseeds but little information exists on the effective use of SS for lactation cows. It was hypothesised that a diet supplemented with an Iranian SS variety (IL-111) in combination with fish oil (FO) would result in higher concentrations of trans-18:1 (including vaccenic acid) and conjugated linoleic acids in milk fat than feeding an unsupplemented control diet. Our objective was to determine the effects of feeding diets containing: (i) Control: (C); (ii) 25 g of roasted SS IL-111 (RSS); (iii) 20 g FO and (iv) 25 g RSS + 10 g FO (RSS + FO) per kilogram of dietary DM on feed intake, ruminal fermentation, milk production and fatty acid profile. Eight multiparous Holstein cows were used in a replicated 4 × 4 Latin square design study. The experiment had four periods of 21 days. Milk Fat percentage was lower (p < 0.01) with FO supplementation and averaged 19.0 and 21.5 g/kg milk with FO and RSS + FO compared with 30.3 and 32.5 g/kg with C and RSS. Feed intake also was lower (p < 0.01) with FO vs. C (23.1 vs. 24.5 kg/day) but feeding RSS resulted in greater feed intake compared with other treatments (26 kg/day). Despite lower feed intake with FO, milk production did not change from controls but feeding RSS + FO resulted in greater milk yield than controls (42.6 vs. 39.3 kg/day). Ruminal pH was greater (p < 0.01) in cows fed FO than other treatments. Supplemental FO alone or in combination with RSS resulted in dramatic increases (p < 0.01) in c9,t11-18:2 in milk fat (12.7 and 13.2 g/day vs. 5.8 and 7.02 with C and RSS). It was surprising to note that 25 g/kg RSS can improve feed intake.     

Effects of replacing fish meal by soybean meal along with supplementing phosphorus and magnesium in diet on growth performance of Persian sturgeon, Acipenser persicus

Looking for replacing fish meal by cheaper and more sustainable protein sources is essential for reducing the cost of fish feeds. Soybean meal is a suitable alternative protein sources for carnivorous fish such as Persian sturgeon, Acipenser persicus. However, it contains antinutritional factors that may affect bioavailability of minerals and lead in reduced growth. Achieving a cost-effective diet that does not have negative effects on growth is the goal of aquaculture programs. A 10-week experiment was conducted with Persian sturgeon (352.07 ± 5.51 g) to determine the combined effects of phosphorous (SP), magnesium and phosphorous (SPMg), phytase (SF), phytase and magnesium (SFMg), phosphorous and phytase (SPF), phosphorous, magnesium and phytase (SPMgF) on weight gain, feed efficiency, specific growth rate, and condition factor. A control diet was prepared with fish meal as a control group. Inclusion of P, Mg, and phytase contents within soybean diets did not improve feed efficiency, and still, the control diet containing fish meal showed better weight gain and feed efficiency. Among soybean meal groups, feed efficiency and specific growth rate were significantly improved for fish fed the diet containing just phytase (SF) and both phytase and phosphorus (P ≤ 0.05). It was true for specific growth rate and condition factor. Phytase significantly enhanced growth whether included with or without phosphorous. This study showed that fish meal is more sufficient for Persian sturgeon, and soybean meal could be partly an alternative protein source if phosphorous supplied for fish by incorporation with microbial phytase or phosphorous.     

Nitrate Removal from Waters Containing Sulfate Using Autotrophic Denitrification with Sulfide Return

In this study, a process has been proposed whereby the sulfide required for autotrophic denitrification is supplied by reducing the sulfate of influent water without the need to add an external sulfide source. The molar ratio of nitrate-to-sulfide was maintained at 1.6. The proposed system was operated continuously for 6 months, including two anoxic and anaerobic reactors with upward flow. The results indicate that the average amount of nitrate declined by 74%. The pH of 7–8 was more effective than a pH of 6 in removing the nitrate. As the hydraulic retention time was prolonged from 1.5 to 3 and was further prolonged to 5 h, the system efficiency was enhanced by removing the nitrate. An alkalinity consumption rate of 1.15 mg (as CaCO₃) per mg of removed NO₃ ^?-N was achieved. In the effluent water, the increased sulfate was 6.7 mg per mg of removed NO^? ₃-N, while the hardness was diminished by 2.85 mg (as CaCO₃).     

Molecular identification of a phytoplasma associated with Russian olive witches’ broom in Iran

Russian olive trees (Elaeagnus angustifolia) showing witches’ broom symptoms typical of phytoplasma infection were observed in the Urmia region of Iran. A phytoplasma named Russian olive witches’ broom phytoplasma (ROWBp-U) was detected from all symptomatic samples by amplification of the 16S rRNA gene and 16S/23S rDNA spacer region using the polymerase chain reaction (PCR) which gave a product of expected length. DNA from symptomless plants used as a negative control yielded no product. The sequence of the 16S rRNA gene and 16S/23S rDNA spacer region of ROWBp-U showed 99% similarity with the homologous genes of members of the aster yellows group. We also detected a phytoplasma in neighboring alfalfa plants (AlWBp-U) showing severe witches’ broom symptoms. An 1107 bp PCR product from the 16S rRNA gene showed 99% homology with the corresponding product in ROWBp-U, suggesting the presence of the same phytoplasma actively vectored in the area. Further observations showed that Russian olive trees with typical ROWB symptoms were present in an orchard near Tehran which is located over 530 km south-east of the original Urmia site. The corresponding sequence of this phytoplasma (ROWBp-T) showed 99% homology to that of the ROWBp-U. A sequence homology study based on the 16S rRNA gene and 16S/23S rDNA spacer region of ROWBp-U and other phytoplasmas showed that ROWBp-U is most closely related to the 16SrI group. To our knowledge, this is the first report of a phytoplasma infection in a member of the Elaeagnaceae.     

Effects of onion on serum uric acid levels and hepatic xanthine dehydrogenase/xanthine oxidase activities in hyperuricemic rats

The aim of this study was to investigate the effects of onion on serum uric acid levels and hepatic Xanthine Dehydrogenase/Xanthine Oxidase activities in normal and hyperuricemic rats. Hyperuricemia was induced by intraperitoneal injection of 250 mg kg(-1) potassium oxonate in rats. Oral administration of onion at 3.5 and 7.0 mg kg(-1) day(-1) for 7 days was able to reduce serum uric acid levels in hyperuricemic rats with no significant effects on the level of this compound in the normal animals. In addition, onion when tested in vivo on rat liver homogeneities elicited significant inhibitory actions on the Xanthine Dehydrogenase (XDH) and Xanthine Oxidase (XO) activities. This effect resulted less potent than that of allopurinol. However, the hypouricemic effect observed in the experimental animal did not seem to parallel the change in XDH and XO activities, implying that the onion might be acting via other mechanisms apart from simple inhibition of enzyme activities. Such hypouricemic action and enzyme inhibitory activity of onion makes it a possible alternative for allopurinol, or at least in combination therapy to minimize the side-effects of allopurinol, in particular in long-term application.     

Humoral and cellular immune responses against Type I cysteine proteinase of Leishmania infantum are higher in asymptomatic than symptomatic dogs selected from a naturally infected population

Canids are natural reservoirs of Leishmania infantum and have been promoted as experimental hosts to decipher the pathogenesis of human visceral leishmaniasis (VL). In this study, the presence of IgG antibodies as well as the presence of mononuclear leukocytes reactive to different cysteine proteinases (CPs) were examined in 13 L. infantum-infected dogs (six with symptoms, seven asymptomatic). Cysteine proteinases which belong to papain-like enzymes known as clan CA are the most studied CPs of parasite protozoa. These molecules are expressed by the intracellular stages of the parasite and could be immunogenic. We studied Type II CP (CPA) and Type I CP (CPB) with its long C-terminal extension (CTE) which could be highly immunogenic. We showed that the level of antibodies reactive to rCPA is low in both symptomatic and asymptomatic dogs. In contrast, when CPB and CTE were used as antigens, the level of total IgG (with IgG2 superior to IgG1) reached higher values in asymptomatic dogs than in dogs with VL. While the peripheral blood mononuclear cell (PBMC) reactivity was significant when cultured in the presence of freezed/thawed (F/T) lysate, it remained low in presence of CP although always higher for PBMC recovered from asymptomatic dogs. We showed the importance of CPB and CTE in particular as a target of immune response and their potential use for serodiagnosis in asymptomatic dogs.     

The pathology of goat paratuberculosis: gross and histopathological lesions in the intestines and mesenteric lymph nodes

From a pathological examination of the intestinal tracts of 1590 goats killed at slaughterhouses in the Fars Province of Iran, 59 cases (3.71%) were suspected, on gross examination, of having paratuberculosis. The diagnosis was confirmed by histopathological study and Ziehl-Neelsen staining of direct smears of rectal faeces. On the basis of severity of involvement of the terminal ileum and mesenteric lymph nodes, the microscopic lesions were classified to mild, moderate and severe forms. Caseous necrosis and calcification were observed only in the mesenteric lymph nodes. High numbers of acid-fast organisms were present in the epithelioid macrophages of the intestine but were inapparent or sparse in the mesenteric lymph nodes. On microscopic examination, 13.5% of the suspected animals were found to have paratuberculosis, in comparison with 3.38% by direct faecal smears. In addition, 30.5% and 15.3% of the animals were diagnosed as having eosinophilic enteritis and linguatulosis, respectively. These findings stress the importance of a careful histopathological examination of the intestines and mesenteric lymph nodes for the diagnosis of paratuberculosis in goats.     

Production of the Malawi chambo, Oreochromis karongae (Trewavas, 1941) association, and Oreochromis shiranus (Boulenger, 1896) in polyculture with the African catfish, Clarias gariepinus (Burchell, 1822)

Results are reported of the growth and yield of the Malawi chambo, Oreochromis karongae (Trewavas, 1941) association, and Oreochromis shiranus (Boulenger. 1896) in polyculture with Clarias gariepinus (Burchell. 1822). Six ponds of 150 m² were assigned to two replicates of three treatments: C. gariepinus and O. shiranus; C. gariepinus and chambo spawned on station; C. gariepinus and chambo collected from the lake. Total fish stocking density was 3 m^?2, and stocking ratio 1:2 (C. gariepinus: titapias). Initial mean body weights were 10.9 g for O. shiranus. 13.2 g for C. gariepinus and 13.3 g for chambo. The ponds were fertilized with chicken manure at the rate of 13.5 kg dry matter ha^?1 day^?1, representing the daily NPK loading of 0.618.0.186 and 0.191 kg ha^?1, respectively. Maize bran was applied at 3% body weight day^?1 for 168 days. Fish growth rate for C. gariepinus was significantly higher (P < 0.05) than that of the tilapias. Weight gains were 0.48-0.57 g day^?1 for C. gariepinus, 0.30-0.31 g day^?1 for chambo and 0.24 g day^?1 for O shiranus. There was no difference (P > 0.05) in the growth of chambo despite the source of fingerlings. More fish biomass came from C. gariepinus stocked at 1 m^?2 than from the tilapias stocked at 2 m^?2. Extrapolated fish yields in the range of 4.37-4.75 t ha^?1 year^?1 were obtained from the polyculture of chambo and C. gariepinus. Oreochromis shiranus and C. gariepinus gave 3.95 t ha^?1 year^?1. Low water temperatures for 112 days slowed the growth of tilapias but did not seem to inhibit the growth of C. gariepinus. Chicken manure and maize bran, applied at smallholder farmer's level, has produced higher yields than by using O. shiranus in monoculture at optimal temperatures. The yields obtained show that the polyculture system is feasible during the cold season and may justify investment in aquaculture at semi-intensive level in Malawi.