Effect of Citrate Concentration on the Extraction Efficiency of Selected Micronutrients from Soil

This study was carried out with the objective of evaluating the effect of citrate concentration on the extraction efficiency of some micronutrients from soil. Composite surface soil samples (0–20 cm) were collected from Eastern Harage Zone (Babile and Haramaya Districts), Wolaita Zone (Damot Sore, Boloso Bombe, Damot Pulasa and Humbo Districts) and Dire Dawa Administrative Council in purposive sampling. The treatments were arranged in completely randomized design (CRD) with three replications. A greenhouse pot experiment with soybean plant was conducted to determine the correlation between soil test methods and the selected micronutrients, such as iron (Fe), copper (Cu), manganese (Mn) and zinc (Zn) in the leaves of the plant. The results showed that, among the different citrate concentrations with strontium chloride (SrCl₂) tested for the determination of available Fe, Mn and Zn, the highest correlation coefficients (r = 0.82, p < 0.05), (r = 0.96, p < 0.001) and (r = 0.98, p < 0.001) were found between the diethylenetriamine pentaacetic acid (DTPA) method and 0.02 M strontium chloride (SrCl₂)-0.025 M citric acid extractant, respectively. Therefore, 0.02 M SrCl₂-0.025 M citric acid extractant is considered to be the most effective for the determination of Fe, Mn and Zn in soils of the studied areas. Similarly, high correlation coefficients (r = 0.97, p < 0.001) were found between DTPA and 0.02 M SrCl₂-0.05 M citric acid and (r = 0.88, p < 0.01) between DTPA and 0.02 M SrCl₂-0.025 M citric acid extractants for the determination of available Cu from soils. Hence, the 0.02 M SrCl₂-0.05 M citric acid extractant was shown to be the best for the determination of Cu in soils of the studied areas. However, considering the use of universal extractant, the 0.02 M SrCl₂-0.025 M citric acid extractant could easily be adopted as a procedure for the determination of Fe, Cu, Mn and Zn for both agricultural and environmental purposes. The greenhouse experiment confirmed the result.     

The effect of potassium on yield,nutrient uptake and efficiency of teff (Eragrostis tef Zucc. Trotter) on vertisols of North Western Ethiopian Highlands

Vertisols are characterized by deficiency of nutrients and recently, potassium (K), a major plant nutrient in crops, is gaining attention because of crop removal, fixation by clay minerals and leaching. A field experiment was conducted during the 2015 and 2016 main cropping seasons to test the effect of potash fertilizer on Vertisols of East Gojjam at Gudalima and Dejen/Tik sites using teff crop. The K rates (applied as muriate of potash) were 0, 50,100, and 150?kg ha^?1. The experiment was laid out in a randomized complete block design in three replications. The results indicated that the plant height, panicle length, number of effective tillers, dry matter and grain yield of teff increased significantly (P?<?0.05) with applied K. The highest dry matter and grain yield (6966.4 and 2418.2 kg ha^?1, respectively) were obtained from the application of 100 kg ha^?1 KCl. Total uptake of N, P, and K were enhanced significantly with K treated plots than those without and K efficiency was improved due to the rate of K. The present study demonstrated the importance of K application to supplement NPS for optimum dry matter and grain yield of teff on Vertisols of the study sites.     

Long-Term Phosphorus Desorption Using Dialysis Membrane Tubes Filled with Iron Hydroxide and its Effect on Phosphorus Pools

Many agricultural fields that have received long-term applications of phosphorus (P) often contain levels of P exceeding those required for optimal crop production. Knowledge of the effect of the P remaining in the soil (residual effect) is of great importance for fertilization management. Plant P availability of residual P in soils is usually estimated using successive cropping experiments carried out in field or greenhouse studies. As this approach is very expensive and time consuming, more rapid soil test methods that can approximate this biological measure are required. The objective of this paper was to use a different approach to evaluate P availability (desorption) over a long period of time instead of the classical means of extraction. Thus, a modified sequential P extraction procedure using dialysis membrane tube filled with ferric hydrate solution (DMT-HFO) was used on the long-term P fertilized soils that received differential P treatments (PoLo, P₁L₁ and P₂L₁) to determine the changes in the different P pools and to relate these P fractions with maize yield. In this study, the contribution of both the labile and non-labile Pi fractions in replenishing the solution Pi was significant where as the organic fractions appeared to have limited contributions in replenishing the solution P. Highly significant correlations were observed between dry matter yield and the P pools extracted by HFO-P_i (0.997*), HCO₃-P_i (r = 0.994**), OH-P_i (r = 0.969**), OH-P_o (r = 0.944**), D/HCl-Pi (0.991**), and C/HCl-P_i (r = 0.997**). Strongly significant correlations were also observed between the different P fractions and plant P uptake. The C/HCl-Pi was the fraction that decreased most especially for the high P treatments indicating that this fraction contributed significantly to the P extracted by DMT-HFO. This suggested that this fraction might be a buffer to more labile P fractions. The combined method employed here could act as an analytical tool to approximate successive cropping experiments carried out under green house condition. But the applicability of this method at a field level should also be assessed. Data from a wider range of soils is also needed to evaluate the universality of this method.     

Genetic diversity of African wild rice (Oryza longistaminata Chev. et Roehr) at the edge of its distribution

Ethiopia is at the edge of the distribution for African wild rice, Oryza longistaminata. Here, chloroplast (cp) and simple sequence repeat (SSR) markers were applied to wild rice accessions in Ethiopia to evaluate how they differ from control O. longistaminata, O. barthii and O. glaberrima accessions which originated from African countries. Based on the cp genomes of African wild rice species, maternally inherited cpINDEL markers were developed. The cp indels helped to elucidate 20 plastid types. African cultivated rice shared a particular plastid type with one of annual O. barthii. Parts of northern wild rice in Ethiopia shared Type 6 with control O. longistaminata. The north group shared another type with parts of the south group. The 16 SSR markers amplified a total of 155 alleles in 215 rice accessions, with mean allelic richness of 9.688 per locus, observed heterozygosity of 0.241, expected heterozygosity of 0.724, polymorphic information content of 0.700, and a significant genetic differentiation of 0.215. Both cpINDEL and nuclear markers analyses suggested that wild rice in Ethiopia belongs to O. longistaminata. However, they carry both a unique plastid type and different population structure from control O. longistaminata collected from other areas in Africa. We concluded that the edge of its distribution maintains unique variation. These populations are regarded as valuable genetic resources for future rice breeding.

     

Application of Neutron Activation Analysis Technique for the Analysis of Soil Samples from Farmlands of Yebrage Hawariat,East Gojjam,Ethiopia

Farmers may not be conscious for their farmland＇s nutrients, soil organic matter, water and air because they simply concerned only for their labor availability and soil fertility losses. The composition and proportion of these components greatly influence soil physical properties, including texture, structure and porosity, the fraction of pore space in a soil. The soil of this farmland must be able to supply adequate amount of plant nutrients, in forms which can be absorbed by the crop, within its lifespan. Deficiencies or imbalances in the supply of any of essential elements can compromise growth, affecting root development, cell division, crop quality, crop yield and resistance to disease and drought. This study was conducted to fill this knowledge gap in order to develop economically vital and environmentally accepted nutrient management strategies for the use of soils in agricultural lands. The objective of this study is to assess the elemental contents and concentration of soil samples collected from farmlands of ＂Yebrage＂ using neutron activation analysis （NAA） techniques regardless of oxidation state, chemical form or physical locations. NAA is used to determine the elemental composition and concentrations present in a soil. The macro/micronutrient and organic matter deficiencies have been verified in agricultural soils through increased use of soil testing and plant analysis. The challenge for agriculture over the coming decades will meet the world＇s increasing demands for food in a sustainable way. Current issues and future challenges point out that as long as agriculture remains a soil based industry, major decreases in productivity likely to be attained ensuring that plants do not have adequate and balanced supply of nutrients.