甜椒始花期氮素分配动态的研究

 利用¹⁵N 示踪技术研究了营养液培养甜椒始花期吸收的氮素在体内的动态分配规律。结果表明: 根与果实中氮的含量在始采期以后保持稳定; 而叶片中氮的含量则随生育进程迅速下降, 盛采期时与果实和根相近, 且两倍高于茎和侧枝。始花期由根吸收的标记氮主要贮存在叶片中, 2 周后向新生器官的运转率高达50. 6%, 4 周后达到57. 1%, 6 周后为58. 0%, 说明越是新近吸收的氮素越容易被再度运转到其它器官, 随着在体内时间的延长, 氮的再运转能力逐渐降低。开花后2 周收获的果实中标记氮占始花期吸收总量的3. 24%, 第3、4、6 周分别为11. 12%, 9. 49%和4. 75%。果实是甜椒体内氮的强力库, 氮素竞争力最强。     

沼泽地改造实验的调查与研究

调查研究结果表明，实验所采取的各项措施是可行有效的，沼泽地生境发生了明显变化，地下水平均下降０．６－１．０ｍ，地温５－２０ｃｍ处提高６－８℃，土壤的理化性质得到明显改善，水、气、热趋于协调。林木生长良好，６年生落叶松树高、胸径生长量分别为０．４６ｍ和０．５０ｃｍ，已达到速生丰产林标准。     

Growing situation of afforested trees and damage to lower-story trees caused by felling in multi-storied forest management in Peninsular Malaysia

The growing condition of afforestation trees and damage to lower-story trees caused by felling and hauling of upper-story trees in a multi-storied forest in Peninsular Malaysia was surveyed. Seventy months have passed since the planting ofAcacia mangium upperstory trees, which have grown 233 m³/ha in volume. The growth ofShorea leprosula thirty-five months after planting of lower-story trees have reached 5.54 m in height. However, as the ratio of height to basal diameter exceeds 100:1, trees are tapering to a considerable degree. A backhoe was installed in order to fell the upper-story trees. From the results of logging, the damage ratio estimation of the lower-story trees was simulated by using the Monte Carlo method. The damage ratio differed considerably depending on planting patterns, height of upper-story trees and felling skills. The results of this survey proved that the felling and hauling of upper-story trees would be technically feasible in the future, except when one row upper-story trees and one row lower-story trees method were employed.     

Improvement of Wheat Water Use Efficiency in Semiarid Area of China

The greatest fear of global climate change is drought since in most areas where wheat is grownwater is the most important factor influencing wheat yield. Average wheat yield throughout the world is only30 -60% of the attainable yield potential because water shortage is the major factor preventing the realizationof maximum yield. Periods of drought alternating with short periods of available water are common conditionsto influence wheat productivity. Such conditions include variable frequency of dry and wet periods, intensityof drought, rate of drought onset and patterns of soil water deficit and/or atmospheric water deficit. It is thisdeficit and variable water conditions in semiarid environments that influence wheat productivity variously.This paper reviewed the physiological adaptation and benefits associated with deficit and variable water condi-tions. In addition, it also highlights the compensative effect of limited irrigation and breeding of new varietiesfor high water use efficiency (WUE) that could improve wheat productivity under water-limited environmentsin the semiarid regions. Considerable potential for further improvement in wheat WUE and productivity insemiarid environments seems to depend on effective conservation of moisture and effici ent use of this limitedwater such as soil fertility improvement, conservation tillage, residues and film mulch, rain water harvestingfor limited irrigation, and breeding for water saving varieties. Different crop, soil and water managementstrategies should be adjusted according to the conditions that prevail in various semiarid areas. By combiningsoil and water conservation approaches and adjusting the cropping system by growing drought-tolerant and wa-ter-saving cultivars, increase in wheat WUE and productivity could be achieved.     

Method for rapid detection of the PvCesA3 gene allele conferring resistance to mandipropamid, a carboxylic acid amide fungicide, in Plasmopara viticola populations

BACKGROUND: The occurrence of carboxylic acid amide (CAA)‐fungicide‐resistant Plasmopara viticola populations is becoming a serious problem in the control of grapevine downy mildew worldwide. RESULTS: The authors have developed a method, which utilises PCR‐RFLP, for the rapid detection of resistance to the CAA fungicide mandipropamid in P. viticola populations. With this method, a glycine‐to‐serine substitution at codon 1105 of the cellulose synthase gene PvCesA3 of CAA‐fungicide‐resistant P. viticola was easily detected, although no resistant P. viticola was detected from 398 isolates in Japan. CONCLUSION: It is proposed that the PCR‐RFLP method is a reliable tool for the rapid detection of CAA‐fungicide‐resistant P. viticola isolates. Only 4 h was required from the sampling of symptoms to the phenotyping of fungicide resistance. Copyright © 2011 Society of Chemical Industry     

河北省黑龙江地区旱地小麦生产限制因子分析

本文研究了1989-1993年不同降水年型(干旱、正常、湿润)条件下灌水和施肥对黑龙港地区旱作小麦产量的影响，结果表明，在正常和湿润年份，土壤贫瘠是限制产量的主要因子，施肥可以大幅度提高产量。但在干旱年份，土壤水分缺乏制约产量的增加，一次灌水可使产量翻一番，在灌一次水的基础上，每公顷施入100公斤氮和70公斤磷，产量则可以再翻一番。可见，增加肥料投入是旱作小麦产量提高的重要手段之一。     

Allelopathy variation in dryland winter wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.) accessions grown on the Loess Plateau of China for about fifty years

In this study, we investigated the genetic variation of allelopathic potential, its grey correlation with important agronomic characters, and rank analysis on allelopathic stability of fifteen wheat accessions commonly grown in arid regions of the Loess Plateau in China. The genetic variation of allelopathic property in dryland winter wheat showed significant differences between accessions. Allelopathic effects exhibited high heritability (55–95%) throughout the life cycle of wheat. Heritability was highest in the tillering stage and weakest in the seed filling stage. Allelopathic potential varied and was discontinuous throughout the wheat life cycle. Grey correlation analysis indicated a close relationship between allelopathic potential dependant on growth phase and agronomic characters. Allelopathic expression during some growth periods induced a partial correlation effect on some important agronomic characters that affected wheat yield. Allelopathic heritability and its degree of influence on yield were more evident in the vegetative growth stage compared to the reproductive stage. A multiple linear regression was built between allelopathic potential during different growth periods and agronomic characters pursued in wheat breeding. Allelopathic potential had a linear effect on production traits cultivated in wheat breeding. When allelopathic intensity varied from 0 to 1 in the reproductive stage, plant height ranged from 44 to 108 cm, spike length from 6.4 to 9.2 cm, number of spikelets with seeds from 13.4 to 21.0, mean seed number per spike from 41.5 to 50.3 and thousand seed weight from 36.2 to 38.3 g. Based on rank analysis, we concluded that there was a synergistic relationship between allelopathic potential in wheat and genetic, chemical and ecological factors. Triticum aestivum L. ‘No 6 Lankao’ and T. aestivum L. ‘No 22 Xiaoyan’ were identified as stable and relatively strong allelopathic accessions, whereas T. aestivum L. ‘Lankao 95–25’ was a stable but relatively weak allelopathic cultivar. Other varieties showed unstable allelopathic potential.     

Enzyme-linked immunosorbent assay for detection of canine chromogranin A by use of immunological cross-reactivity of rabbit anti-bovine chromogranin A antibody

Bovine and canine chromogranin A were extracted and purified from each specie's adrenal glands. Isolated bovine 70 kDa protein showed 100% identity to bovine CgA reported previously, whereas isolated canine 68 kDa protein showed 83.3% identity to bovine CgA by the NH(2)-terminal amino acid sequence analysis. Rabbit antibody to purified bovine protein (CgA) was found to immunologically cross-reacted with purified canine protein (CgA). In sandwich ELISA with anti-bovine CgA, concentration-dependent curves were obtained ranging from 0.3 to 20 mug/ml for canine CgA. From these findings, sandwich ELISA with anti-bovine CgA is found to be useful to determine the concentration of canine CgA.     

Passive transfer of antibodies to Shiga toxin-producing Escherichia coli O26, O111 and O157 antigens in neonatal calves by feeding colostrum

To study whether or not passive immunity of neonatal calves against Shiga toxin-producing Escherichia coli (STEC) O26, O111, and O157 was obtained by colostrum administration, serum antibodies in calves after the feeding were determined by enzyme-linked immunosorbent assay (ELISA) in comparison with antibodies in colostrum and sera from donor dams. The highest antibody titers to STEC in colostrum from dams were detected soon after parturition. The antibody titers were found to be elevated in sera of neonatal calves (4-9 hr after birth) orally administered with colostrum with high antibody titers, suggesting that passive immunity of neonatal calves to STEC infection may be obtained by feeding colostrum. These results suggest that colostrum administration to neonatal calves may play an important role in elevating serum antibodies against STEC in neonatal calves.     

Antigenic differences between H5N1 human influenza viruses isolated in 1997 and 2003

To assess whether the antigenic properties of H5 hemagglutinin (HA) change over time due to antigenic drift, we produced a panel of monoclonal antibodies (mAbs) against the HA of the index H5N1 human influenza A virus, A/Hong Kong/156/97. By immunizing mice with a plasmid expressing this HA and boosting the initial immunization with cell lysates transfected with the plasmid, a total of six hybridomas producing HA-specific mAbs were established: four to the HA1 subunit with hemadsorption-inhibiting activity and two to the HA2 subunit. None of the mAbs to HA1 could bind to the HA of a recent human isolate, A/Hong Kong/213/2003, indicating that there are substantial antigenic differences between the H5N1 human influenza virus isolated in 1997 and that isolated in 2003.