Antitumor activity of compounds isolated from leaves of Eriobotrya japonica

In a search for possible antitumor agents from natural sources, megastigmane glycosides and polyphenolic constituents isolated from the leaves of Eriobotrya japonica (Rosaceae) were found to inhibit the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced activation of Epstein-Barr virus early antigen in Raji cells. Roseoside and procyanidin B-2 were among the active compounds found in an in vitro assay; these compounds were further assessed for antitumor activity in vivo in a two-stage carcinogenesis assay on mouse skin. Roseoside significantly delayed carcinogenesis induced by peroxynitrite (initiator) and TPA (promoter), and its potency was comparable to that of a green tea polyphenol, (-)-epigallocatechin 3-O-gallate, in the same assay.     

小麦籽粒钙元素含量的研究进展

Increasing the mineral content is becoming the important research direction and major target for crops breeding in the world. Calcium is an essential mineral element for human health and plays a pivotal role in skeletogenesis and metabolism. It is estimated that about 3.5 billion people was suffered from calcium deficiencies. Calcium deficiency has become a major international problem harming human health. The staple food is an optimal and safe way to mineral supplement. Wheat, one of the main food crops in China and even in the world, is the main source of food for 35%-40% of global population, a main source for human’s calcium intake as well as an important crop of mineral element biofortification. Improving the calcium content in wheat grains through genetic improvement is considered to be the most economical, effective and sustainable measure to solve the calcium deficiency, which has aroused great concern from international scholars. This paper summarized the recent advances in the study of calcium content in wheat grains, mainly including the genetic variation, affecting factors, the relationship with related traits and regulation mechanism of calcium content in grain. Furthermore, we also put forward the direction of future research on calcium-fortified wheat, which provides solutions for accelerating the effective calcium supplementation through staple food, promoting the healthy and nutritious dietary pattern, ensuring the food security to meet the transition from “quantitative” to “qualitative” demands, improving people’s health, and reducing economic losses caused by calcium deficiency.     

基于动态模拟模型分析冬小麦干物质积累特征对产量的影响

为揭示冬小麦产量形成与干物质积累的定量关系,2009—2011年利用3个田间试验建立了不同播种期、基本苗、底肥用量、追氮时期和用量、冬前灌水量、返青后灌水量和灌水时期等的冬小麦群体,分析了冬小麦干物质积累过程特征对产量及其构成因素的影响机制和定量关系。冬前干物质积累特征用单株干物重和冬前茎蘖数来表示,越冬后特征由基于相对积温(R_GDDi)的Logistic曲线特征来描述。结果表明,群体干物质积累最大速率、干物质积累速率的2个拐点等特征量与产量及其构成因子有紧密的联系,所建立的关系模型均达到极显著水平(P<0.01)。对有关模型进一步用独立样本进行配对t-检验,结果模拟值与实际值差异不显著(P>0.05)。基于相对积温(R_GDDi)的Logistic模型不仅能较好地描述小麦冬后干物质积累过程,而且其曲线特征也能用于分析冬小麦产量及其构成因素的形成规律,以此为基础的产量关系方程可用于冬小麦生产群体调控的理论分析。     

Polyketides from a Marine-Derived Fungus Xylariaceae sp.

Eighteen polyketides (1–18) including six citrinin derivatives, two phenol derivatives, one cyclopentenone, two naphthol derivatives, and seven tetralone derivatives were isolated from the culture broth of a marine-derived fungal strain Xylariaceae sp. SCSGAF0086. Five of these compounds (1, 2, 8, 9, and 10) were new, and their structures were determined by spectroscopic methods. Compounds 4, 6, 7, and 17 showed enzyme-inhibitory activities towards several tested enzymes, and 6 and 7 showed strong antifouling activity against Bugula neritina larvae settlement. This is the first time that the antifouling and enzyme-inhibitory activities of these compounds has been reported.     

冬小麦低温处理叶片细胞膜透性的QTL定位

为探索冬小麦抗寒性的分子机制,以168个花培3号×豫麦57的双单倍体株系为作图群体,利用已构建含有324个SSR标记的遗传图谱,对电导法测定低温(−18℃)处理后的叶片膜透性进行QTL定位。利用完全区间作图法,在3种环境下共检测到21个与叶片膜透性相关的加性QTLs,分布于1B、2A、3A、3B、5B、6A、6B、6D、7B和7D染色体上,其中4个位点(qCMP-1B-1、qCMP-3B-2、qCMP-5B-1和qCMP-5B-4)遗传贡献率大于10%,属主效基因,其余QTL的遗传贡献率较小,属微效基因。3种环境条件下在5B染色体的Xgwm213–Xswes861.2区间检测到共同位点,与Xswes861.2的遗传距离为0 cM,其中qCMP-5B-1 (环境1)和qCMP-5B-4 (环境3)的贡献率高达17.5%和14.0%。研究结果对于小麦抗寒标记选择和抗寒育种具有应用价值。     

河北省农业环境面源污染现状及影响因素与对策建议

河北省是全国重要农产品生产基地之一,保护农业环境和水资源是河北省支撑京津冀协同发展的必然选择。通过分析河北省农业环境污染的现状以及影响因素,针对性地提出了河北省防控农业环境面源污染的建议。     

Isoquinoline Alkaloids as Protein Tyrosine Phosphatase Inhibitors from a Deep-Sea-Derived Fungus Aspergillus puniceus

Puniceusines A–N (1–14), 14 new isoquinoline alkaloids, were isolated from the extracts of a deep-sea-derived fungus, Aspergillus puniceus SCSIO z021. Their structures were elucidated by spectroscopic analyses. The absolute configuration of 9 was determined by ECD calculations, and the structures of 6 and 12 were further confirmed by a single-crystal X-ray diffraction analysis. Compounds 3–5 and 8–13 unprecedentedly contained an isoquinolinyl, a polysubstituted benzyl or a pyronyl at position C-7 of isoquinoline nucleus. Compounds 3 and 4 showed selective inhibitory activity against protein tyrosine phosphatase CD45 with IC₅₀ values of 8.4 and 5.6 µM, respectively, 4 also had a moderate cytotoxicity towards human lung adenocarcinoma cell line H1975 with an IC₅₀ value of 11.0 µM, and 14, which contained an active center, -C=N⁺, exhibited antibacterial activity. An analysis of the relationship between the structures, enzyme inhibitory activity and cytotoxicity of 1–14 revealed that the substituents at C-7 of the isoquinoline nucleus could greatly affect their bioactivity.     

西南喀斯特山区农果草系统耦合及生产效益分析——以广西平果县果化镇龙何屯为例

本研究设计通过生态功能的转换,引入高效生态农业技术,提出了山顶优良树种封植、山腰果草 果药套种、山洼地粮果(草)结合、庭院畜牧圈养和半圈养、联动节水的水分贮存和输送灌溉网的农业生态系统空间发展的农果草耦合模式,引入地草轮作技术、果草(药)复合技术、秸草秆垫圈沤肥技术、石山人工草地的建植和管理技术、以及石山区水资源开发和节水灌溉技术等关键技术。通过家畜消耗农田输出的作物籽粒、饲草等,实现饲草生产供应与家畜生长需求的平衡配置;同时利用家畜圈肥调节玉米和果树地、草地的物质循环与能量流动,调控农果草地的耦合生产,最大限度提升岩溶峰丛洼地区域土地资源的综合利用效率,以获取最佳生态和经济效益。该研究经济效益成效显著,如火龙果(投产第1年)套种板兰根收入可达44775元/hm2,比单纯种植玉米和黄豆增收260.5%,而火龙果套种牧草,再耦合养山羊,则收入可达60330元/hm2,比玉米大豆耦合养猪增收230.2%。     

基于土壤传递函数的土壤持水特性间接估计及其空间变异性研究

A total of 107 soil samples were taken from the city of Qingdao,Shandong Province,China.Soil water retention data at 2.5,6,10,33,100,300,and 1 500 kPa matric potentials were measured using a pressure membrane apparatus.Multiple linear regression (MLR) was used to develop pedotransfer functions (PTFs) for single point estimation and van Genuchten parameter estimation based on readily measurable soil properties,i.e.,MLR-based point (MLRP) PTF and MLR-based parametric (MLRV) PTF.The double cross-validation method was used to evaluate the accuracy of PTF estimates and the stability of the PTFs developed in this study.The performance of MLRP and MLRV PTFs in estimating water contents at matric potentials of 10,33,and 1 500 kPa was compared with that of two existing PTFs,the Rawls PTF and the Vereecken PTF.In addition,geostatistical analyses were conducted to assess the capabilities of these PTFs in describing the spatial variability of soil water retention characteristics.Results showed that among all PTFs only the Vereecken PTF failed to accurately estimate water retention characteristics.Although the MLRP PTF can be used to predict retention characteristics through traditional statistical analyses,it failed to describe the spatial variability of soil water retention characteristics.Although the MLRV and Rawls PTFs failed to describe the spatial variability of water contents at a matric potential of 10 kPa,they can be used to quantify the spatial variability of water contents at matric potentials of 33 and 1 500 kPa.     

Territrem and Butyrolactone Derivatives from a Marine-Derived Fungus Aspergillus Terreus

Seventeen lactones including eight territrem derivatives (1–8) and nine butyrolactone derivatives (9–17) were isolated from a marine-derived fungus Aspergillus terreus SCSGAF0162 under solid-state fermentation of rice. Compounds 1–3 and 9–10 were new, and their structures were elucidated by spectroscopic analysis. The acetylcholinesterase inhibitory activity and antiviral activity of compounds 1–17 were evaluated. Among them, compounds 1 and 2 showed strong inhibitory activity against acetylcholinesterase with IC₅₀ values of 4.2 ± 0.6, 4.5 ± 0.6 nM, respectively. This is the first time it has been reported that 3, 6, 10, 12 had evident antiviral activity towards HSV-1 with IC₅₀ values of 16.4 ± 0.6, 6.34 ± 0.4, 21.8 ± 0.8 and 28.9 ± 0.8 μg·mL⁻¹, respectively. Antifouling bioassay tests showed that compounds 1, 11, 12, 15 had potent antifouling activity with EC₅₀ values of 12.9 ± 0.5, 22.1 ± 0.8, 7.4 ± 0.6, 16.1 ± 0.6 μg·mL⁻¹ toward barnacle Balanus amphitrite larvae, respectively.