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1.
Light microscopic observations revealed that in camel foetuses of 25 mm crown‐to‐rump length (CRL) the primordial tubular system of the prospective lung was formed of several tubules lined by undifferentiated columnar epithelium. Intra‐epithelial neuroendocrine cells were the first elements to be differentiated in the lining epithelium of the primordial tubular system of the prospective lung as early as 25 mm CRL. On reaching 50–67 mm CRL, the primordial tubular system started to differentiate into two systems of primordial tubules, the prospective bronchial or light tubules and the future respiratory or dark tubules. The lining epithelium of the prospective bronchial tubules revealed a clear evidence of ciliogenesis as early as 80 mm CRL. From 800 mm CRL onwards, the bronchial epithelium demonstrated ciliated and non‐ciliated secretory cells. The non‐ciliated secretory cells of the bronchial epithelium of fetal camel lung showed moderate reaction to AB/PAS technique, for the first time, in fetuses reaching 600 mm CRL. 相似文献
2.
Effects of five cryoprotectants on proliferation and differentiation‐related gene expression of frozen‐thawed bovine calf testicular tissue
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Hao Li Yi‐Lin Bian Nicola Schreurs Xiao‐Gang Zhang Sayed Haidar Abbas Raza Qian Fang Li‐Qiang Wang Jian‐Hong Hu 《Reproduction in domestic animals》2018,53(5):1211-1218
The cryopreservation of testicular tissue is a potential method for preserving male fertility. However, the effect of cryopreservation on bovine calf testicular tissue is scarce. This study investigated the effect of different cryoprotectants on bovine calf testicular tissue at the molecular level. Testicular tissue from ten immature bovine calves (6 months) was collected after slaughter and cryopreserved in an extender containing different concentrations of the following five cryopreservation solutions (CP): bovine serum albumin (BSA) with 5% dimethyl sulfoxide (DMSO), trehalose with 5% DMSO, DMSO and glycerol and ethylene glycol (EG). After 7‐day cryopreservation, the expression levels of three spermatogonial stem cell (SSC)‐related genes, octamer‐4 (OCT4), KIT ligand (MGF/SCF) and kit oncogene (C‐KIT), were investigated by quantitative PCR (qPCR). The cell viability was highest for the tissues preserved with 30 mg/ml BSA (77.82% ± 1.22) and 40 mg/ml trehalose (74.23% ± 1.16) compared with other groups (p < 0.05), and the level of expression of the three genes was highest with 30 mg/ml BSA (p < 0.05). Compared with other CPs, the 30 mg/ml BSA and 40 mg/ml trehalose have the better cryopreserve protection. The 30 mg/ml BSA is the most viable media for the cryopreservation of testicular tissue from cattle. 相似文献
3.
Ali Asghar Naghipour Seyed Jamaleddin Khajeddin Pejman Tahmasebi Majid Iravani 《African Journal of Range and Forage Science》2016,33(1):67-71
Fire products have been widely identified as germination cues for a number of species from both fire-prone and fire-free ecosystems. However, there is uncertainty about the effects of fire products on seed germination of semi-arid rangeland species. We examined the germination of seven dominant species from Central Zagros rangelands in the semi-arid region of Iran under various heat shock (60, 80 and 120 °C), aerosol smoke, ash and control treatments. Our findings showed that fire products could enhance, inhibit or not affect the seed germination of rangeland species in this area. The germination percentage of Astragalus adscendens increased following 60 °C heat treatment in comparison with the control. The germination of Astragalus verus, Bromus tectorum, B. tomentellus and Phlomis olivieri were increased significantly using smoke treatment (P<0.0001). The seed germination of B. tomentellus and B. tectorum was promoted by ash treatment, whereas the other species exhibited no response or were inhibited by fire products. In addition, smoke treatment shortened the overall germination time of B. tectorum. These findings suggest that fire products affect the germination of several species and can serve as potential tools for understanding the vegetation dynamics and restoration of disturbed semi-arid rangelands. 相似文献
4.
Mai H. ElNaggar Ghada M. Abdelwahab Omnia Kutkat Mohamed GabAllah Mohamed A. Ali Mohamed E. A. El-Metwally Ahmed M. Sayed Usama Ramadan Abdelmohsen Ashraf T. Khalil 《Marine drugs》2022,20(3)
Several natural products recovered from a marine-derived Aspergillus niger were tested for their inhibitory activity against SARS CoV-2 in vitro. Aurasperone A (3) was found to inhibit SARS CoV-2 efficiently (IC50 = 12.25 µM) with comparable activity with the positive control remdesivir (IC50 = 10.11 µM). Aurasperone A exerted minimal cytotoxicity on Vero E6 cells (CC50 = 32.36 mM, SI = 2641.5) and it was found to be much safer than remdesivir (CC50 = 415.22 µM, SI = 41.07). To putatively highlight its molecular target, aurasperone A was subjected to molecular docking against several key-viral protein targets followed by a series of molecular dynamics-based in silico experiments that suggested Mpro to be its primary viral protein target. More potent anti-SARS CoV-2 Mpro inhibitors can be developed according to our findings presented in the present investigation. 相似文献
5.
Hani A. Alhadrami Gaia Burgio Bathini Thissera Raha Orfali Suzan E. Jiffri Mohammed Yaseen Ahmed M. Sayed Mostafa E. Rateb 《Marine drugs》2022,20(3)
The COVID-19 pandemic and its continuing emerging variants emphasize the need to discover appropriate treatment, where vaccines alone have failed to show complete protection against the new variants of the virus. Therefore, treatment of the infected cases is critical. This paper discusses the bio-guided isolation of three indole diketopiperazine alkaloids, neoechinulin A (1), echinulin (2), and eurocristatine (3), from the Red Sea-derived Aspergillus fumigatus MR2012. Neoechinulin A (1) exhibited a potent inhibitory effect against SARS-CoV-2 Mpro with IC50 value of 0.47 μM, which is comparable to the reference standard GC376. Despite the structural similarity between the three compounds, only 1 showed a promising effect. The mechanism of inhibition is discussed in light of a series of extensive molecular docking, classical and steered molecular dynamics simulation experiments. This paper sheds light on indole diketopiperazine alkaloids as a potential structural motif against SARS-CoV-2 Mpro. Additionally, it highlights the potential of different molecular docking and molecular dynamics simulation approaches in the discrimination between active and inactive structurally related Mpro inhibitors. 相似文献
6.
W. Mubarak M. N. Saleh R. Sayed A. Ohtsuka T. Taguchi T. Murakami 《Anatomia, histologia, embryologia》2005,34(S1):34-35
Perineuronal nets (PNs) of condensed extracellular matrix (ECM) have been shown to characterize the microenvironment of individual neurons and the chemoarchitecture of some brain regions. In the present study, PNs in the hippocampus were visualized with a cationic iron colloid method for sulphated proteoglycan content and a plant lectin from Vicia villosa agglutinin (VVA) for N-acetylgalactosamine containing glycoconjugates. The ECM molecules were organized in reticular coats (PNs) around non-pyramidal cells in the Ammons horn (Corneu Ammons, CA) and subicular region, in addition to pyramidal neurons located in CA2 and CA3 regions. CA2 stratum pyramidale exhibited the most intense staining of its PNs and a diffuse intervening neutrophil labelling, while CA3 region showed a graded fashion of staining intensity. The subiculum displayed intensely stained perineuronal coats. Notably, the hippocampal perineuronal nets revealed overlapped staining characteristics with both staining methods. However, cell coats of the subicular neurons showed various degrees of labelling characteristics with both markers. It is suggested that the PNs in the hippocampus are correlated with the fast spiking inhibitory GABAergic neurons and their target pyramidal cells, and this is important to keep the excitatory elements under control and therefore control the information processes within the hippocampus. 相似文献
7.
Follicular epithelium of the thyroid gland of the one-humped camel was examined by light and electron microscopy. It consisted of a single type of epithelial cell which varied from flattened to columnar in shape. Follicular epithelial cells were characterized by the presence of markedly dilated cisternae of rough-surfaced endoplasmic reticulum, well developed Golgi apparatus, abundant small vesicles (150 nm to 200 nm in diameter) in the apical cytoplasm, and electron-dense colloid droplets measuring from 250 nm to 1600 nm. Follicular epithelial cells frequently showed apocrine secretion into colloidal lumens. Apocrine protrusions with smooth surface were dome-like or balloon-like structures and contained a fine granular matrix. These findings indicate that the morphological features of the follicular epithelial cells of the thyroid gland of the camel are essentially similar to those of mammals except for the presence of apocrine secretion, which is unique to the camel. 相似文献
8.
Inter-simple sequence repeat (ISSR) markers were used to study phylogenetic relationships among 33 citrus genotypes including several undefined local or native varieties as well as some known varieties in the Fars Province of Iran. 相似文献
9.
BACKGROUND: Busulfan, a cytotoxic drug, which is currently used as a chemotherapeutic agent, has many side effects on different body organs. In this research, the effects of busulfan on sperm parameters and microstructure of mouse testis were investigated. METHODS: Busulfan was injected intrapretoneally at 10, 20, 30, 40 and 50 mg/kg and testes were removed after 4, 6 and 8 weeks, weighed and processed for light microscopic examination. Transverse and cross section diameters of testes, seminiferous tubules diameters, percentage of different types of tubules, epithelium thickness, spermatogenic cell numbers and capsule thickness as well as the sperm parameters in epididymis were measured. RESULTS: There was a significant decline in sperm numbers and marked changes in testes structures. Almost 8 weeks after the injection of drug, some of the changes are reversed. Accordingly, the changes in percent of normal tubules without sperm, abnormal tubules and capsule thickness were increased until 6 weeks of drug administration, the changes declined thereafter. CONCLUSION: In general, busulfan caused a decrease in all analyzed parameters (except capsule thickness, normal tubules without sperm and abnormal tubules), probably due to the arrest of spermatogenesis. Our results also revealed that some of the changes are reversible and dose dependent. 相似文献
10.
Ahmed Amin Sayed ;Sobhy Ahmed Temerak ;Salah Mahmood Mohmed Gameel ;Amr Moussa 《农业科学与技术》2014,(6):529-534
Alternate two different modes of actions of green chemicals: spinosad & methoxyfenozide to combat the lesser date moth, Batrachedra amydraula and the Almond moth, Cadra cautella Walk on dates in Dakhla Oasis, New Valley, Egypt were done in 2010 and 201 I. Based on infestation of the early insect pest B. amydraula, the three-rotation program with basic 1st spray of spinosad 24 SC at 20 mL/100 L showed insignificant difference. Based on minimum effective rotation doses, alternating spinosad 24 SC at 20 mL/100 L, then methoxyfenozide 24 SC at 15 mL/100 L with two-week interval showed zero infestation and 1.8% at the last sample of 2010 and 2011, respectively. Based on infestation of the late insect pest, Cadra spp., tlhe same previous trend of the three-rotation results. Based on minimum effective rotation doses, alternating spinosad 24 SC at 20 mL/100 L, then methoxyfenozid 24 SC at 15 mL/100 L with three-week interval indicated less than 1% and 1% infestation during harvest for 2010 and 201 l, respectively. The last reflected important value when storing the fruit. Both products proved to be working under dry hot high temperatures. 相似文献