紫甘薯多酚氧化酶性质探讨

甘薯是世界上一种重要的粮食和能源作物,由于加工过程中其组织中的多酚氧化酶易催化多酚类物质氧化成黑色素,严重影响了其加工品质.以济薯18为材料,研究了pH值、温度、底物浓度等对其多酚氧化酶(PPO)活性的影响.结果表明:紫甘薯济薯18中PPO活性在360 am处最大,其最适反应时间为11 min,最适pH值6.5,最适温...     

不同栽培模式下青贮玉米的农艺性状

为探究不同的栽培模式对青贮玉米(Zea mays)农艺性状及产量形成的影响,选用新饲玉19号在(30+60)、(10+66)、(17+50)、(60+60)、(76+76)、(40+60)和(30+90)cm 7种行距栽培模式下对比研究。结果表明,(60+60)cm的栽培模式中青贮玉米株高、穗位高等农艺性状以及单株叶面积、倒四叶SPAD值和单株干鲜重均高于其他栽培模式,随着栽培行距的增加,青贮玉米冠层中下部透光率有所增加,(60+60)cm等行距栽培下青贮玉米冠层结构布局合理,生物量显著高于其他栽培模式(P=0.003)。不同的栽培模式中,随着行距的增加玉米农艺性状表现良好,(60+60)cm等行距栽培时有利于青贮玉米产量的形成。     

Chitosan nanoparticles enhance developmental competence of in vitro-matured porcine oocytes

Oxidative stress is inevitable as it is derived from the handling, culturing, inherent metabolic activities and medium supplementation of embryos. This study was performed to investigate the protective effect of chitosan nanoparticles (CNPs) on oxidative damage in porcine oocytes. For this purpose, cumulus–oocyte complexes (COCs) derived from porcine slaughterhouse ovaries were exposed to different concentrations of CNPs (0, 10, 25 and 50 µg/ml) during in vitro maturation (IVM). Oocytes treated with 25 µg/ml CNPs showed significantly higher levels of GSH, along with a significant reduction in ROS levels compared to control, CNPs10 and CNPs50 groups. In parthenogenetic embryo production, the maturation rate was significantly higher in the CNPs25 group than that in the control and all other treated groups. In addition, when compared to the CNPs50 and control groups, CNPs25-treated oocytes showed significantly higher cleavage and blastocyst development rates. The highest concentration of CNPs reduced the total cell number and ratio of ICM: TE cells in parthenogenetic embryos, suggesting that there is a threshold where benefits are lost if exceeded. In cloned embryos, the CNPs25 group, as compared to all other treated groups, showed significantly higher maturation and cleavage rates. Furthermore, the blastocyst development rate in the CNPs25-treated group was significantly higher than that in the CNPs50-treated group, as was the total cell number. Moreover, we found that cloned embryos derived from the CNPs25-treated group showed significantly higher expression levels of Pou5f1, Dppa2, and Ndp52il genes, compared with those of the control and other treated groups. Our results demonstrated that 25 µg/ml CNPs treatment during IVM improves the developmental competence of porcine oocytes by reducing oxidative stress.     

Cellular Localization of Rice SUMO/SUMO Conjugates and in vitro Sumoylation Using Rice Components

正Many proteins are regulated by post-translational modifications,such as the reversible covalent attachment of ubiquitin and ubiquitin-like proteins in eukaryotes(Kerscher et al, 2006). Post-translational modification of proteins by the SUMO protein family is involved in diverse cellular processes, including development, hormonal responses, and biotic and abiotic stress signaling(Park et al, 2011). SUMO modification can modulate protein-protein interactions, intracellular localization or the activities of the protein(Gareau and Lima, 2010).     

Potential Anti-Aging Substances Derived from Seaweeds

Aging is a major risk factor for many chronic diseases, such as cancer, cardiovascular disease, and diabetes. The exact mechanisms underlying the aging process are not fully elucidated. However, a growing body of evidence suggests that several pathways, such as sirtuin, AMP-activated protein kinase, insulin-like growth factor, autophagy, and nuclear factor erythroid 2-related factor 2 play critical roles in regulating aging. Furthermore, genetic or dietary interventions of these pathways can extend lifespan by delaying the aging process. Seaweeds are a food source rich in many nutrients, including fibers, polyunsaturated fatty acids, vitamins, minerals, and other bioactive compounds. The health benefits of seaweeds include, but are not limited to, antioxidant, anti-inflammatory, and anti-obese activities. Interestingly, a body of studies shows that some seaweed-derived extracts or isolated compounds, can modulate these aging-regulating pathways or even extend lifespans of various animal models. However, few such studies have been conducted on higher animals or even humans. In this review, we focused on potential anti-aging bioactive substances in seaweeds that have been studied in cells and animals mainly based on their anti-aging cellular and molecular mechanisms.     

军犬幼犬阶段培训

一、培养幼犬良好的体质为了保证军犬有健壮的体质和持久的耐力,因此应对幼犬的体质进行科学的锻炼。其方法主要有三方面。1.培养幼犬的耐力素质在初期阶段幼犬5000米有一定速度的跑步,运动分10次进行,每次不少于400米,随着锻炼的深入以后每次不少于800米,在带犬运动时要由慢到     

农业机械化对种植业经济增长贡献的实证分析——以辽宁省为例

在解决"三农"问题和建设新农村过程中,农业机械化的重要作用越来越显著.为此分析了影响农业经济增长的主要因素,运用项目有无法分析模型,测算了2006年农业机械化对辽宁省农业经济增长的贡献率.研究结果表明:农业机械化对农业经济增长的贡献率为20.6%,影响农业经济增长的主要因素有农业生产成本、种植面积、农作物产量和农业机械作业效率等.     

不同改良剂对铅污染砖红壤的修复效果

在未污染的花岗岩砖红壤中加入重金属Pb，平衡两周后在污染土壤上施用不同水平的钙镁磷肥、牛粪、钙镁磷肥＋牛粪等改良剂，以小白菜作为指示作物，根据小白菜生长动态变化和收获后生物量、Pb吸收量、土壤pH、土壤中有效Pb含量等理化性质的差异，来评价各种改良剂对Pb污染砖红壤的改良效果，以便为海南花岗岩砖红壤Pb污染修复提供理论依据。实验结果表明：各改良剂对Pb污染土壤都有一定的修复效果，不同程度的减轻了小白菜的毒害症状，其中钙镁磷肥与牛粪配施处理效果最好，该处理的小白菜不仅生物量高，而且Pb含量较低；其次是钙镁磷肥处理，而改良效果最差的是牛粪，其植株叶片不仅稍有黄化症状，且Pb含量较高     

最小二乘法在水泵试验曲线拟合中的应用

根据目前国内外水泵测试软件中需要曲线拟合的要求,充分考虑了目前水泵试验软件曲线拟合的基础,我们将最小二乘法应用到水泵试验软件中.从软件中实现了流量-扬程曲线的拟合,流量一效率曲线的拟合,流量-功率曲线的拟合,以及流量-汽蚀余量曲线的拟合,并且拟合曲线的多项式方程的次方数和拟合精度可以手动或自动选择,有效避免了目前曲线拟合精度不高的问题.实际试验结果表明将最小二乘法应用在VB开发的水泵测试软件中,绘图准确,曲线美观大方,使用方便,符合国家相关标准的要求,保证了试验的测试精度.     

Variation and correlation analysis of flavonoids and carotenoids in Korean pigmented rice (Oryza sativa L.) cultivars

Flavonoids and carotenoids of pigmented rice ( Oryza sativa L.), including five black cultivars and two red cultivars, from Korea were characterized to determine the diversity among the phytochemicals and to analyze the relationships among their contents. Black cultivars were higher in flavonoids and carotenoids than the red and white cultivars. The profiles of eight phytochemicals identified from the rice grains were subjected to principal component analysis (PCA) to evaluate the differences among cultivars. PCA could fully distinguish between these cultivars. The Heugjinjubyeo (BR-1) and Heugseolbyeo (BR-2) cultivars were separated from the others based on flavonoid and carotenoid concentrations. Flavonoid contents had a positive correlation with carotenoid contents among all rice grains. The BR-1 and BR-2 cultivars appear to be good candidates for future breeding programs because they have simultaneously high flavonoid and carotenoid contents.