Forest Type,Diversity and Biomass Estimation in Tropical Forests of Western Ghat of Maharashtra Using Geospatial Techniques

Tropical forests are the world’s largest terrestrial storehouses of carbon and are recognized as rich, diverse and highly productive ecosystems. The present study was conducted to characterize the land use, diversity and biomass of tropical forest in Western Ghat of Maharashtra State in India through satellite remote sensing and GIS. The study has been designed and implemented to promote analysis on Western Ghat biodiversity resources including trees, shrubs and herbs based on inventorying, monitoring and mapping. Field measured biomass is integrated with spectral responses of various bands and indices of the Landsat TM satellite image for estimation of above-ground biomass in a 36,046 km² area of relic forest in the Central Western Ghat. The above-ground biomass from field-based inventory varied from 30.2 to 151.1 ton/ha in moist deciduous forest, 9.2–99.1 ton/ha in dry deciduous forest, 42.1–158.6 ton/ha in semi-evergreen forest, and 160.9–271 ton/ha in evergreen forest. The total above-ground biomass of the study area was estimated to be 95.2 M tons. A regression equation between field above-ground biomass and a Normalized Difference Vegetation Index was used for spectral modeling to estimate and prepare the above-ground biomass map in the region. A total 120 plant species in 81 genera and 31 families were identified in the study area. This study emphasizes the importance of relic forests for their biodiversity, carbon sequestration and total biomass.     

Changes in transition: technology adoption and rice farming in two Indian villages

The economic impacts of the Green Revolution have been studied widely, but not its social-cultural effects on different farming communities. The adoption of high yielding varieties (HYVs) of rice changed the nature of rice farming in the two West Bengal villages of Padulara and Naigachi. The villages present an interesting contrast of socio-economic and cultural change due to the differences in the level of adoption of agricultural technologies. This study documents the social and cultural impacts of agricultural technology adoption, specifically the effect on rituals which guided the stages of traditional rice farming and communal life. Agricultural rituals are being modified to suit the processes of modern rice farming, while family rituals are holding strong. The study also shows the evolving nature of rituals as it reflects new found wealth, gender roles, and economic class in these villages.     

Molecular evidence for zoonotic transmission of Giardia duodenalis among dairy farm workers in West Bengal, India

No study in the past has examined the genetic diversity and zoonotic potential of Giardia duodenalis in dairy cattle in India. To assess the importance of these animals as a source of human G. duodenalis infections and determine the epidemiology of bovine giardiasis in India, fecal samples from 180 calves, heifers and adults and 51 dairy farm workers on two dairy farms in West Bengal, India were genotyped by PCR-RFLP analysis of the β-giardin gene of G. duodenalis followed by DNA sequencing of the nested PCR products. The overall prevalence of G. duodenalis in cattle was 12.2% (22/180), the infection being more prevalent in younger calves than in adult cattle. Zoonotic G. duodenalis Assemblage A1 was identified in both calves and workers although the most prevalent genotype detected in cattle was a novel Assemblage E subgenotype. These findings clearly suggest that there is a potential risk of zoonotic transmission of G. duodenalis infections between cattle and humans on dairy farms in India.     

Differential inhibition of digestive proteases by tannin in two size groups of rohu (Labeo rohita,Hamilton): A biochemical and zymography study

This study was intended to evaluate tannin mediated inhibition of digestive proteases in two different size groups (fingerlings, F, 4.5 ± 0.7 cm; advanced fingerlings, AF, 18.2 ± 1.6 cm) of rohu, Labeo rohita. Graded levels (50, 100, 150 and 200 nM) of tannin (Gallotannin, 99% purity) were added to the enzyme extracts (30°C, 1 hr) prior to determination of enzyme activities. Changes in the activity of trypsin, chymotrypsin and total protease in relation to the control sets were determined through biochemical assay of enzymes and SDS‐PAGE zymography. The study revealed that tannin significantly inhibited trypsin, chymotrypsin and total protease activities in a dose dependent manner as evident from the regression equations. The degree of the inhibition appeared to be significantly higher for the F in contrast to the AF (F_5,66 = 282.311; p < 0.0001). Trypsin activities were reduced by 8.04 ± 0.19% to 52.68 ± 0.72% and 5.61 ± 0.22% to 39.46 ± 0.19% in F and AF, respectively. The reduction in Chymotrypsin activities ranged between 16.11 ± 0.03% to 38.02 ± 0.27% in F and 6.31 ± 0.07% to 22.80 ± 0.32% in AF. Total protease activities were reduced by 10.9 ± 0.07% to 49.60 ± 0.32% in L. rohitaF, whereas, it ranged between 5.19 ± 0.06% to 32.60 ± 0.13% in AF. On a comparative scale, the difference in tannin induced inhibition in F and AF were more prominent for trypsin and chymotrypsin than total protease. Further, nine protease activity bands (15.9–69 kD) with different electromobility were noticed in both the size groups. Subsequent densitometry analysis revealed that the average densities of the protease activity bands were gradually decreased with increasing level of tannin exposure (50–200 nM). The study might indicate adaptive tolerance to tannin in larger size groups and emphasizes the need for removal of tannin in the plant feedstuffs, especially for feeding the fingerlings of L. rohita.