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排序方式: 共有239条查询结果,搜索用时 15 毫秒
1.
Sakamoto K Yano T Kobayashi T Hagino A Aso H Obara Y 《Domestic animal endocrinology》2007,32(4):260-272
Growth hormone (GH) plays a specific role to inhibit apoptosis in the bovine mammary gland through the insulin-like growth factor (IGF)-I system, however, the mechanism of GH action is poorly understood. In this study, we show that GH dramatically inhibits the expression of IGFBP-5, and GH along with IGF-I enhanced the phosphorylation of Akt through the reduction of IGF binding protein (IGFBP)-5. To determine how GH affects Akt through IGF-I in bovine mammary epithelial cells (BMECs), we examined the phosphorylation of Akt in GH treated BMECs and found that IGF-I induced phosphorylation of Akt was significantly enhanced by the treatment with GH. We demonstrated that GH reduces mRNA and protein expression of IGFBP-5 in BMECs, but it does not affect the expression of IGFBP-3. To determine that the enhanced effect of the Akt phosphorylation by the treatment of GH is due to the inhibition of the expression of IGFBP-5, we examined the effect of IGFBP-3 and -5 on the phosphorylation of Akt through IGF-I in the GH-treated BMECs. The phosphorylation of Akt was inhibited in a dose-dependent manner when IGFBP-5 was added at varying concentrations and was also inhibited in the presence of IGFBP-3. The results of this study suggest that GH plays an important role on mammary gland involution in bovine mammary epithelial cells. 相似文献
2.
We investigated the turnover time of microbial biomass-C in Japanese upland soils with various textures and examined the soil physicochemical properties influencing their turnover time. Samples from five different soil types (sand-dune regosol, light-colored Andosol, humic Andosol, brown forest soil, and dark red soil) were taken from upland concrete-frame plots in the experimental field of Chiba University. Each soil amended with [U -13C] glucose was incubated for 80 d at 25°C. Microbial biomass-C and -13C in soil were periodically determined by the fumigation-extraction method. The longest turnover time of microbial biomass-C was observed in the dark red soil (215 d) followed by the humic Andosol (134 d), brown forest soil (97 d), and light-colored Andosol (83 d) and the shortest in the sand-dune regosol (45 d). The turnover time of microbial biomass-C was significantly correlated with the value of soil clay (R: 0.917*), CEC (R: 0.921*), and macroaggregate (R: 0.907*) contents, but not with the total-C content. The amount of microbial biomass-C showed a close correlation with the turnover time of microbial biomass-C, suggesting that the turnover time of microbial biomass-C is an important factor influencing the accumulation of microbial biomass-C in soil. 相似文献
3.
Konomi Sakamoto Wataru Honto Masaharu Iguchi Nobuhiro Ogawa Kazuhiro Ura Yasuaki Takagi 《Fisheries Science》2009,75(1):91-98
This study examines the following in the Japanese mitten crab: (1) the structure of the exoskeleton with special reference
to its calcification; (2) the progression of post-molt cuticle formation and calcification. In the crab, the structure and
calcification state of the exoskeleton at the molt and during the inter-molt stage were similar to those of other crustaceans.
During the inter-molt, the exoskeleton consisted of four cuticle layers; the outermost epicuticle, the exocuticle, the endocuticle
and the innermost membrane layer. Intense calcification was observed in the exo- and endocuticle. At the molt, the synthesis
of the epi- and exocuticle was already complete, and the addition of the endocuticle began after the molt. Calcification of
the exocuticle initiated soon after the molt, but there was a delay between endocuticle matrix synthesis and calcification.
Histology showed that the process of calcification was similar to that in other crustaceans. However, calcium concentrations
within the exoskeleton continued to increase and never reached the levels of the inter-molt stage at the end of the experiment.
This suggests that the Japanese mitten crab is relatively slow to calcify compared to other crustaceans. 相似文献
4.
Sakamoto K Lewbart GA Smith TM 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2001,30(2):50-52
Abstract: Blood samples were collected from 29 juvenile red pacu ( Piaractus brachypomus ), ornamental freshwater fish, to establish baseline blood chemistry values. Mean (minimum-maximum) values, obtained by automated bichromatic analysis and ion selective electrode analysis, were as follows: sodium, 150.4 (146–159) mmol/L; potassium, 3.93 (2.7–5.0) mmol/L; chloride, 138.7 (128–150) mmol/L; total CO2 , 7.5 (6–10) mmol/L; albumin, 0.86 (0.5–1.0) g/dL; lactate dehydrogenase, 237.8 (65–692) IU/L; aspartate aminotransferase, 49.1 (0–125) IU/L; creatinine, 0.31 (0.2–0.4) mg/dL; calcium, 10.80 (9.5–12.5) mg/dL; anion gap, 6.89 (1.2–12.5) mmol/L; and phosphorus, 7.29 (4.1–8.9) mg/dL. 相似文献
5.
Ishikawa A Sakamoto H Katagiri S Takahashi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(1):99-102
The longitudinal changes in fecal steroid hormone concentrations and sexual behavior in 2 mated/pregnant and 3 non-mated female Hokkaido brown bears were investigated during the breeding season. Behavioral estrus (standing) lasted for 14 and 32 days in the mated females and for 25 to 36 days in the non-mated females. In non-mated females, sexual behavior, such as female-female mounting and masturbation, was observed for several days before and after the estrous period. In mated females, mean fecal estradiol-17beta concentrations were higher in the estrous period than in the post-estrous period, while fecal progesterone concentrations were higher in the post-estrous period than in the estrous period. The similar trends of steroid hormone changes were observed in the non-mated females. 相似文献
6.
Effects of propofol-sevoflurane anesthesia on the maternal and fetal hemodynamics blood gases, and uterine activity in pregnant goats 总被引:2,自引:0,他引:2
Setoyama K Shinzato T Misumi K Fujiki M Sakamoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1075-1081
To determine the effects of propofol and sevoflurane on hemodynamics, acid-base balance and uterine activity in pregnant animals, a prospective experimental study was designed by use of ten pregnant goats. Propofol was intravenously administered at a bolus dose of 5 mg/kg and then infused a rate of 0.3 mg/kg/min for 5 min. Following the induction, the animals were incrementally inhaled 2.7 and 4.1% of end-tidal concentration of sevoflurane each for 30 min, and then recovered. The maternal and fetal heart rate (HR), arterial blood pressure (BP) and acid-base balance, the intrauterine pressure (IUP), and the uterine blood flow (UBF) were measured. Following the pre-anesthetic data, the parameters were measured 7 times throughout the anesthetic and recovering periods. The propofol infusion induced 1.37 times of HR increase and produced decrease in PO(2) and a relevant metabolic acidemia in the mother, with no effect in the fetus. Sevoflurane reduced BP in the fetus from 30 (2.7%) to 60 (4.1%) min of inhalation. The uterine contractions disappeared throughout sevoflurane inhalation, and then recurred within 15 min after the cessation of sevoflurane. Propofol injection increases HR, and induces a moderate hypoxemia and metabolic acidemia associated with the suppressed ventilation for pregnant goats, with less effect on the fetal hemodinamics. Sevoflurane causes minimal change in maternal hemodynamics, but induces significant hypotension in the fetus and reduction of uterine activity. These data may be useful in making anesthetic choices combined with analgesia for Caesarian section in goats. 相似文献
7.
Setoyama K Ota H Miura N Fujiki M Misumi K Sakamoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):499-503
Milrinone, a therapeutic agent for acute congestive heart failure, has both inotropic and vasodilatory effects, but investigations of these effects of milrinone were almost all conducted under normoxia, and few reports have investigated how milrinone affects the hemodynamics and redistribution of regional blood flow under severe hypoxia. By using colored microspheres, we investigated how milrinone affects hemodynamics and the redistribution of regional blood flow under severe hypoxia. Twelve healthy mongrel dogs were divided into 2 groups. The milrinone group was infused with milrinone cumulatively at 25, 75 and 250 microg/kg for 5 min each. The intact group was infused with saline instead of milrinone. We measured the hemodynamics and cerebrum, cerebellum and kidney blood flow in both groups. Both groups were inspired with 10% oxygen. Milrinone induced significant decrease in mean pulmonary artery and pulmonary vascular resistance, compared with the intact group. In both groups slight decreases in mean arterial pressure, systemic vascular resistance and double-product were seen. In regional blood flow, milrinone-induced increases in blood flow were seen in the cerebrum, cerebellum, and especially in the kidneys. Milrinone's vasodilatory effects were sufficient even under hypoxia. And milrinone increased regional blood flow slightly in the cerebrum and cerebellum, and significantly in the kidneys. These results suggested that milrinone protects against hypoxia-induced organ damage especially in the kidneys. In addition, milrinone is very potent in improving severe congested hemodynamics which complicates hypoxic pulmonary vasoconstriction. 相似文献
8.
Newcastle disease virus (NDV), named MET95, was isolated from a non-vaccinated broiler flock in Japan in 1995. The MET95 strain was determined to be a lentogenic NDV. The strain has the properties of eluting rapidly at 4 C and has low thermostability in hemagglutinating activity with chicken erythrocytes. In these studies, no difference could be found between the MET95 strain and the Hitcher B1 vaccine strain. However, the chickens inoculated with the MET95 strain, as well as chickens that they were in contact with, had a much higher hemagglutination-inhibition antibody response than those inoculated with the B1 strain. Accordingly, the MET95 strain is thought to be a promising candidate as a live ND vaccine strain. In Japan, this is the first report on the isolation of lentogenic NDV from chickens since the paper on the Ishii strain isolated in 1966. 相似文献
9.
Uyanga Ariya Ken-Ya Hamano Takushi Makimoto Shu Kinoshita Yasuaki Akaji Yuko Miyazaki Muneto Hirobe Keiji Sakamoto 《Journal of Forest Research》2016,21(2):73-83
Dendrochronological approaches enable us to understand forest stand dynamics by estimation of disturbance history and age structure. The present study was conducted in an old-growth beech forest in a forest reserve in western Japan. Increment cores were taken for tree ring analysis from all canopy trees in a 50 m × 130 m study plot. Radial growth release criteria were developed to identify significant growth releases in each tree ring series and to characterize the disturbance history of the study site. The age structure of the forest was indicative of continuous establishment by Fagus crenata and simultaneous establishment by Magnolia obovata. A variety of low-intensity disturbances were identified in each decade, especially after the 1900s, but the occurrence of high-intensity catastrophic disturbance was rare, and likely played an important role in maintaining species diversity in the existing forest canopy. The results also suggest that F. crenata regenerates gradually before and after both large- and small-scale disturbances, whereas M. obovata and Betula grossa regenerate only after large-scale catastrophic disturbances. 相似文献
10.
Naoko?MikiEmail author Kosei?Otsuki Keiji?Sakamoto Takashi?Nishimoto Ken?Yoshikawa 《Journal of Forest Research》2003,8(3):153-161
To elucidate the differences in the leaf water relations of Pinus densiflora Sieb. et Zucc. growing in different soil moisture conditions, we examined the pressure-volume curve and the diurnal changes in the stomatal conductance, the transpiration rate, and the leaf water potential. The leaf water relations were compared using field-grown 40-year-old pine trees growing on the upper and lower parts of a slope. We also compared the leaf water relations of potted 4-year-old saplings growing at pF 4.2 and pF 1.8 soil moisture levels for almost 1 year. The values of the ratio of symplasmic water at turgor loss point to symplasmic water at saturated point (Vp/Vo) and bulk modulus of elasticity () of both the adult trees on the upper part of the slope and the potted saplings growing on pF 4.2 soil moisture were higher than those values of both the adult trees on the lower part of the slope and the potted saplings growing on pF 1.8 soil moisture, respectively. The field-grown adult tree and the potted saplings growing under long-term water stress tended to reduce their stomatal conductance in response to the acute soil drying. It is suggested that P. densiflora growing under long-term water stress rapidly closed its stomata in response to soil drying and avoided losing water, and could also rapidly absorb water with reducing water loss because of the decrease in the leaf pressure potential derived from the high values. 相似文献