Soil salinity related to physical soil characteristics and irrigation management in four Mediterranean irrigation districts

Irrigated agriculture is threatened by soil salinity in numerous arid and semiarid areas of the Mediterranean basin. The objective of this work was to quantify soil salinity through electromagnetic induction (EMI) techniques and relate it to the physical characteristics and irrigation management of four Mediterranean irrigation districts located in Morocco, Spain, Tunisia and Turkey. The volume and salinity of the main water inputs (irrigation and precipitation) and outputs (crop evapotranspiration and drainage) were measured or estimated in each district. Soil salinity (EC_e) maps were obtained through electromagnetic induction surveys (EC_a readings) and district-specific EC_a-EC_e calibrations. Gravimetric soil water content (WC) and soil saturation percentage (SP) were also measured in the soil calibration samples. The EC_a-EC_e calibration equations were highly significant (P < 0.001) in all districts. EC_a was not significantly correlated (P > 0.1) with WC, and was only significantly correlated (P < 0.1) with soil texture (estimated by SP) in Spain. Hence, EC_a mainly depended upon EC_e, so that the maps developed could be used effectively to assess soil salinity and its spatial variability. The surface-weighted average EC_e values were low to moderate, and ranked the districts in the order: Tunisia (3.4 dS m⁻¹) > Morocco (2.2 dS m⁻¹) > Spain (1.4 dS m⁻¹) > Turkey (0.45 dS m⁻¹). Soil salinity was mainly affected by irrigation water salinity and irrigation efficiency. Drainage water salinity at the exit of each district was mostly affected by soil salinity and irrigation efficiency, with values very high in Tunisia (9.0 dS m⁻¹), high in Spain (4.6 dS m⁻¹), moderate in Morocco (estimated at 2.6 dS m⁻¹), and low in Turkey (1.4 dS m⁻¹). Salt loads in drainage waters, calculated from their salinity (EC_dw) and volume (Q), were highest in Tunisia (very high Q and very high EC_dw), intermediate in Turkey (extremely high Q and low EC_dw) and lowest in Spain (very low Q and high EC_dw) (there were no Q data for Morocco). Reduction of these high drainage volumes through sound irrigation management would be the most efficient way to control the off-site salt-pollution caused by these Mediterranean irrigation districts.     

The southern route "out of Africa": evidence for an early expansion of modern humans into Arabia

The timing of the dispersal of anatomically modern humans (AMH) out of Africa is a fundamental question in human evolutionary studies. Existing data suggest a rapid coastal exodus via the Indian Ocean rim around 60,000 years ago. We present evidence from Jebel Faya, United Arab Emirates, demonstrating human presence in eastern Arabia during the last interglacial. The tool kit found at Jebel Faya has affinities to the late Middle Stone Age in northeast Africa, indicating that technological innovation was not necessary to facilitate migration into Arabia. Instead, we propose that low eustatic sea level and increased rainfall during the transition between marine isotope stages 6 and 5 allowed humans to populate Arabia. This evidence implies that AMH may have been present in South Asia before the Toba eruption.     

The essential oil of Eucalyptus tereticornis, and its constituents α- and β-pinene,potentiate acetylcholine-induced contractions in isolated rat trachea

The effects of the essential oil of Eucalyptus tereticornis (EOET), especially the effects of its constituents α- and β-pinene, were studied on rat trachea in vitro. In tracheal rings, EOET, α- or β-pinene potentiated the contractions induced by acetylcholine (ACh). Contractions induced by K⁺ (60 mM) were also potentiated by α- and β-pinene, but were reduced by EOET. Our findings show that EOET has myorelaxant effects on rat airways, but potentiates ACh-induced contractions. Monoterpenes α- and β-pinene are involved in its potentiating actions, but are not responsible for its myorelaxant effects. A putative inhibition of the acetylcholinesterase enzyme is involved.     

Development of EST-SSR markers and construction of a linkage map in faba bean (Vicia faba)

To develop a high density linkage map in faba bean, a total of 1,363 FBES (Faba bean expressed sequence tag [EST]-derived simple sequence repeat [SSR]) markers were designed based on 5,090 non-redundant ESTs developed in this study. A total of 109 plants of a ‘Nubaria 2’ × ‘Misr 3’ F₂ mapping population were used for map construction. Because the parents were not pure homozygous lines, the 109 F₂ plants were divided into three subpopulations according to the original F₁ plants. Linkage groups (LGs) generated in each subpopulation were integrated by commonly mapped markers. The integrated ‘Nubaria 2’ × ‘Misr 3’ map consisted of six LGs, representing a total length of 684.7 cM, with 552 loci. Of the mapped loci, 47% were generated from multi-loci diagnostic (MLD) markers. Alignment of homologous sequence pairs along each linkage group revealed obvious syntenic relationships between LGs in faba bean and the genomes of two model legumes, Lotus japonicus and Medicago truncatula. In a polymorphic analysis with ten Egyptian faba bean varieties, 78.9% (384/487) of the FBES markers showed polymorphisms. Along with the EST-SSR markers, the dense map developed in this study is expected to accelerate marker assisted breeding in faba bean.     

Adaptation progressive à la luzerne d'un isolat de Verticillium dahliae, originaire de la tomate

Un isolat de Verticillium dahliae provenant de la tomate s'est montré apte à vivre en parasite aux dépens de la luzerne connue par être attaquée uniquement par Verticillium alboatrum. Cette aptitude parasitaire ne s'accompagne d'aucune manifestation pathologique à la première inoculation. Des passages successifs de cet isolat chez la luzerne ont conduit à des lignées d'aptitudes parasitaire et pathogène nouvelles. Une réduction graduelle de son pouvoir pathogène à l'encontre de son hôte d'origine a été observée contrairement aux aptitudes parasitaires qui se sont maintenues. Les passages successifs sur la luzerne ont également entraîné des variations morphologiques qui ont touché la croissance radiale, le taux de sporulation et la forme de résistance. Des hypothèses sur la nature de ces variations sont proposées et les conséquences agronomiques de ces phénomènes sont discutées.     

First report of Pythium diclinum in Morocco

Pythium diclinum was isolated from a reservoir near the city of Rabat (Morocco). This is the first report of its presence in this country. Taxonomic and morphological details of the fungus are presented. The isolate grew on potato carrot agar (PCA) containing various concentrations of NaCl, up to 700 m m . Pathogenicity of the Moroccan isolate was demonstrated in vitro on lucerne seeds and seedlings.     

Biochemical Responses to True and Bicarbonate-Induced Iron Deficiency in Grapevine Genotypes

ABSTRACT

Biochemical responses to direct or bicarbonate-induced iron (Fe) deficiency were compared in two Tunisian native grapevine varieties, Khamri (tolerant) and Balta4 (sensitive), and a tolerant rootstock, 140Ru. Woody cuttings of each genotype were irrigated with a nutrient solution containing one of the following: 20 μM Fe (control), 1 μM Fe (direct Fe-deficiency), or 20 μM Fe + 10 mM HCO₃ ^? (indirect bicarbonate-induced Fe-deficiency). Under direct Fe-deficient conditions, lower leaf chlorosis score and higher chlorophyll and leaf Fe contents were found in Khamri and 140Ru compared with Balta4. Moreover, indirect Fe deficiency caused similar effects on these parameters, which were more pronounced in Balta4. Both tolerant genotypes, Khamri and 140Ru, showed higher roots-acidification capacity and phenol release under the direct Fe deficiency compared with the bicarbonate-induced condition. In the sensitive variety, Balta4, no significant changes were found between the control and Fe-deficient plants. Root Fe(III)-reductase activity was strongly stimulated by both types of Fe deficiency in Khamri and 140Ru, and displayed no significant changes in Balta4. In the three genotypes, root and leaf activities of two Fe-containing enzymes, catalase and guaiacol peroxidase, were significantly affected under Fe deficiency (either direct or induced), though to a greater extent in the sensitive variety, Balta4. The latter also displayed higher leaf malonyldialdehyde (MDA) content, traducing an important membrane lipid peroxidation.     

ROLE OF SALICYLIC ACID IN REGULATION OF CADMIUM TOXICITY IN WHEAT (TRITICUM AESTIVUM L.)

The present study is focused on the possible mediatory role of salicylic acid (SA) under cadmium (Cd⁺²) toxicity. Cadmium treatments resulted in the inhibition of root dry biomass, root elongation and increased Cd accumulation in roots. Pretreatment of seeds with SA (500 μM) for 20 hrs resulted in protection against Cd, increased root dry biomass, root elongation and minimal accumulation of cadmium. Cadmium chloride (CdCl₂; 0.0, 100, 400 and 1000 μM) produced a significant inhibition in chlorophyll, photosynthetic efficiency [carbon dioxide (¹⁴CO₂)-fixation], relative water content (RWC) and abscisic acid (ABA) content. Pretreatment of seeds with SA alleviated the Cd negative effect on these parameters. A drop in the activities of carboxylating enzymes, phosphoenol pyruvate carboxylase (PEPC, EC 4.1.1.31), and ribulose1,5-bisphosphate carboxylase (RuBPC, EC 4.1.1.39) were observed in Cd treated plants. Pretreatment with SA ameliorated the inhibitory effect of Cd on the studied enzymatic activities. Cadmium applied to salicylic acid free plants increased the level of stress metabolites, malondialdehide (MDA), hydrogen peroxides, and free proline content. Salicylic acid pretreatment decreased the toxic effect level of cadmium manifested by lower lipid peroxidation, lesser production of H₂O₂ and reduction in free proline content. The ultrastructural investigation of the root cells of wheat exposed to different concentrations of Cd for 15 days was carried out. The results indicated that Cd induced several obvious ultrastructural changes such as increased vacuolation, condensed cytoplasm with increased density of the matrix, reduction of mitochondrial cristae, severe plasmolysis, highly condensed nuclear chromatin and increment of disintegrated organelles. Electron dense granules appeared between the cell wall and plasmalemma. In vacuoles, electron dense granules encircled by the membrane were aggregated and formed into larger precipitates, which increase in number and volume as a consequence of excessive Cd exposure. An important issue arising from this study was how the short-term treatment with SA affected several physiological processes, such as plant growth, photosynthesis, and antioxidant defense system. We assume that the beneficial effect of SA during an earlier growth period could be related to avoidance of cumulative damage upon exposure to cadmium. One of the important roles of SA in inducing resistance to various environmental stresses is manifested by its ability to express genes that code for pathogenesis-related proteins or defense-related enzymes. Also, SA could form a complex with Cd that may provide Cd tolerance.     

Carbon content variation in boles of mature sugar maple and giant sequoia

At present, a carbon (C) content of 50% (w/w) in dry wood is widely accepted as a generic value; however, few wood C measurements have been reported. We used elemental analysis to investigate C content per unit of dry matter and observed that it varied both radially and vertically in boles of two old-growth tree species: sugar maple (Acer saccharum Marsh.) and giant sequoia (Sequoiadendron giganteum (Lindl.) Bucholz). In sugar maple there was considerable variation in tree ring widths among four radii for particular annual layers of xylem, revealing that the annual rate of C assimilation differs around the circumference and from the base of each tree to its top, but the observed variation in C content was unrelated to diameter growth rate and strongly related to the calendar year when the wood was formed. Carbon content in sugar maple wood increased in an approximately linear fashion, from < 50 to 51% from pith to cambium, at both the base and top of the boles. In giant sequoia, C was essentially constant at > 55% across many hundreds of years of heartwood, but it declined abruptly at the sapwood-heartwood boundary and remained lower in all sapwood samples, an indication that heartwood formation involves anabolic metabolism. Factors that may be responsible for the different C contents and trends with age between sugar maple and sequoia trees are considered. Tree-ring data from this study do not support some of the key assumptions made by dendrochronology.     

Total, particulate organic matter and structural stability of a Calcixeroll soil under different wheat rotations and tillage systems in a semiarid area of Morocco

Wheat production in Morocco is constrained by both scarce climate and degraded soil quality. There is an urgent need to revert production decline while restoring country’s soils. Among conservation tillage systems known for their improvement in yield, no-till technology was found to influence soil quality as well. Soil quality indices are also affected by wheat rotations at medium and long-terms. This paper discusses changes in selected properties of a Calcixeroll soil, including total and particulate soil organic matter (SOM), pH, total N and aggregation, subjected, for 11 consecutive years, to various conservation and conventional agricultural systems. Tillage systems included no-tillage (NT) and conventional tillage (CT). Crop rotations were continuous wheat, fallow–wheat, fallow–wheat–corn, fallow–wheat–forage and fallow–wheat–lentils. Higher aggregation, carbon sequestration, pH decline and particulate organic matter (POM) buildup are major changes associated with shift from conventional- to NT system. Better stability of aggregates was demonstrated by a significantly greater mean weight diameter under NT (3.8 mm) than CT system (3.2 mm) at the soil surface. There was 13.6% SOC increase in (0–200 mm) over the 11-year period under NT, while CT did not affect much this soil quality indicator. Another valuable funding is the stratification of SOC and total nitrogen in NT surface horizon (0–25 mm) without their depletion at deeper horizon compared to tillage treatments. Fallow–wheat system resulted in reduction of SOC compared to WW, but 3-year wheat rotation tended to improve overall soil quality. Benefits from crop rotation in terms of organic carbon varied between 2.6 and 11.7%, with fallow–wheat–forage exhibiting the maximum. Combined use of NT and 3-year fallow rotation helped to improve soil quality in this experiment.