Detection of Candida species by nested PCR method in one-humped camels (Camelus dromedarius)

Tropical Animal Health and Production - Systemic fungal diseases are the infections caused by false treatment protocols and generally are not taken into consideration especially in the veterinary...     

A comparative study on stability and decay resistance of some environmentally friendly fire-retardant boron compounds

Environmental concern accelerated development of new wood preservatives having low mammalian toxicity in addition to high protective effectiveness. PHN 130 and PHN 130 G have been developed recently by KOHMIX Ltd. in Japan as environmentally safe, fire-proof boron containing compounds. Beyond their proved fire-proof properties, stability of loaded chemical in wood at humid service conditions and decay resistance are required to be known for effective and broad use of these compounds in wood preservation. Results indicated that PHN imparted complete decay resistance to wood against brown- and white-rot fungi Tyromyces palustris and Coriolus versicolor, respectively. Protection efficacy of PHN also continued despite severe weathering conditions provided that impregnation is done by an undiluted solution. PHN 130 G appeared more effective than PHN 130 in this respect. However, both compounds were leachable from treated wood regardless of retention level or physical restriction of water access into wood by compression.This revised version was published online in April 2005 with a corrected date of receipt of the article.

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Clavibacter michiganensis subsp. michiganesis: Tracking strains using their genetic differentiations by ISSR markers in Southern Turkey

There is no available data published related to the dissemination of bacterial canker caused by Clavibacter michiganensis subsp. michiganensis (Cmm) and its genetic diversity in Turkey. It is important to control new introduced inoculum sources by commercial seeds and seedlings. Pathogens were identified by morphological features and the identity was confirmed by PCR amplification using a specific primer PSA-4, PSA-R in addition to microbiological tests. ISSR markers showing high polymorphism ratios were selected and used to characterize Cmm strains. The collected strains were classified into different groups on the basis of ISSR-PCR fingerprints, which showed remarkable genetic specificity and diversity not previously identified in Cmm, suggesting that genetic differences are related to dissemination of the pathogen in the region. This is the first ever study carried out on the characterization of Cmm using ISSR. The selected ISSR primers to characterize Cmm can be used to determine genetic differences in further studies.     

Supercritical CO(2) extraction of beta-carotene and lycopene from tomato paste waste

Lycopene and beta-carotene were extracted from tomato paste waste using supercritical carbon dioxide (SC-CO(2)). To optimize supercritical fluid extraction (SFE) results for the isolation of lycopene and beta-carotene, a factorial designed experiment was conducted. The factors assessed were the temperature of the extractor (35, 45, 55, and 65 degrees C), the pressure of the extraction fluid (200, 250, and 300 bar), addition of cosolvent (5, 10, and 15% ethanol), extraction time (1, 2, and 3 h), and CO(2) flow rate (2, 4, and 8 kg/h). The total amounts of lycopene and beta-carotene in the tomato paste waste, extracts, and residues were determined by HPLC. A maximum of 53.93% of lycopene was extracted by SC-CO(2) in 2 h (CO(2) flow rate = 4 kg/h) at 55 degrees C and 300 bar, with the addition of 5% ethanol as a cosolvent. Half of the initially present beta-carotene was extracted in 2 h (flow rate = 4 kg/h), at 65 degrees C and 300 bar, also with the addition of 5% ethanol.     

The Effect of Kefir as a Dietary Supplement on Nonspecific Immune Response and Disease Resistance in Juvenile Rainbow Trout,Oncorhynchus mykiss (Walbaum 1792)

In this study, juvenile rainbow trout fed with commercial pellets containing kefir provided increased nonspecific immune response and improved disease resistance against lactococcosis and yersiniosis. Kefir was used as a feed supplement at 2, 5, and 10% inclusion rates and several nonspecific immune parameters were observed at day(s) 1, 7, 14, 21, 28, and 35 following the treatment. A total of four experimental groups, including control, was established. The various parameters including hematocrits, nitroblue tetrazolium positive neutrophils, total leukocytes, serum lysozyme activity, total serum protein, and immunoglobulin M (IgM) levels were examined. As a result of this study, kefir‐fed fish had an increase in measured nonspecific immune parameters, especially in the group received the 10% kefir treatment. The challenged fish fed with kefir‐supplemented diet showed a better survival rate against Lactococcus garvieae than Yersinia ruckeri. Kefir supplementation reduced fish mortality significantly against L. garvieae.     

Antimicrobial,Antioxidant, and Antimutagenic Activities of Five Turkish Pear Cultivars

The pear (Pyrus spp.) is one of the most important fruits consumed in daily life. The aim of this study was to determine the total phenolic and ascorbic acid contents, as well as the antimicrobial, antioxidant, and antimutagenic activities, of various pear cultivars. The fruits of five pear (Pyrus communis L.) cultivars (‘Deveci’, ‘Kizil’, ‘Egirsah’, ‘Gugum’, and ‘Banda’) were used in this study. It was determined that the peel and pulp of the ‘Kizil’ pear had the highest total phenolic content (402.5?mg/100?g and 215.2?mg/100?g, respectively), while those of the ‘Banda’ pear had the lowest total phenolic content (326?mg/100?g and 126.1?mg/100?g, respectively). Additionally, the ‘Kizil’ pear showed the highest antioxidant capacity in the 2,2’-azino-bis(3-ethylbenzothiazoline-6-6-sulphonic acid) (ABTS) and ferric-reducing ability assay (FRAP) (1.72 μmol TE/g FW and 161.25?μmol Fe II/g FW, respectively) and the highest ascorbic acid content (16.02?mg/100?g). The ‘Banda’ pear showed the highest antibacterial activity against the test bacteria. However, none of the pear extracts displayed antifungal activity. While all doses of the ‘Kizil’, ‘Gugum’, and ‘Banda’ pear extracts used in this study, except 80?μL/plate, exhibited antimutagenic activities, only the lowest dose (10?μL/plate) of the ‘Deveci’ pear extract showed the antimutagenic activity against induced mutagenesis in the Salmonella typhimurium TA 98 strain. Consequently, the five Turkish pear cultivars used in this study exhibited different levels of antimicrobial, antioxidant, and antimutagenic activities.     

Effect of log seasonality on reproductive potential ofMonochamus galloprovincialis reared in scots pine logs under laboratory conditions

Insect reproduction may be affected by a number of factors including seasonal changes in larval or adult nutrition. The effect of season on the reproductive potential ofMonochamus galloprovincialis (Olivier) females reared inPinus sylvestris L. (Scots pine) logs was investigated by constructing fertility tables for each log that differed only in the season they were cut. Population parameters were compared among three seasonal cohorts. The intrinsic rate of increase and most of the associated population parameters of beetles that emerged from logs cut during spring were higher than for beetles emerged from summer and autumn logs. Slight differences were found between summer and autumn cohorts. We suggest that seasonal differences in the nutritional quality of logs caused differences inM. galloprovincialis survival and reproductive potential. http://www.phytoparasitica.org posting March 16, 2008.     

Effects of oregano essential oil with or without feed enzymes on growth performance,digestive enzyme,nutrient digestibility,lipid metabolism and immune response of broilers fed on wheat–soybean meal diets

1.?The study was conducted to determine the effects of dietary supplementation of enzyme and oregano essential oil at two levels, alone or together, on performance, digestive enzyme, nutrient digestibility, lipid metabolism and immune response of broilers fed on wheat–soybean meal based diets.

2.?The following dietary treatments were used from d 0 to 21. Diet 1 (control, CONT): a commercial diet containing no enzyme or oregano essential oil, diet 2 (ENZY): supplemented with enzyme, diet 3 (EO250): supplemented with essential oil at 250 mg/kg feed, diet 4 (EO500): supplemented with essential oil at 500 mg/kg feed, diet 5 (ENZY + EO250): supplemented with enzyme and essential oil at 250 mg/kg, and diet 6 (ENZY + EO500): supplemented with enzyme and essential oil at 500 mg/kg.

3.?Birds fed on diets containing ENZY, EO250 and ENZY + EO250 had significantly higher weight gain than those given CONT diet from d 0 to 7. No significant effects on feed intake, feed conversion ratio, mortality, organ weights except for jejunum weight and intestinal lengths was found with either enzyme or essential oil, alone or in combination, over the 21-d growth period. The supplementation of essential oil together with enzyme decreased jejunum weight compared with essential oil alone.

4.?Supplementation with enzyme significantly decreased viscosity and increased dry matter of digesta, but did not alter pH of digesta. There was no effect of essential oil alone at either concentration on viscosity, dry matter or pH of digesta. A significant decrease in viscosity of digesta appeared when essential oil was used with together enzyme.

5.?The supplementation of essential oil at both levels with or without enzyme significantly increased chymotrypsin activity in the digestive system, and improved crude protein digestibility.

6.?The higher concentration of essential oil with and without enzyme significantly increased serum total cholesterol concentrations. No significant effect on immune response was found with either enzyme or essential oil, alone or together.

7.?Enzymes and essential oil had different modes of actions. The supplementation of enzyme with essential oil in diets is likely more effective in view of performance, nutrient digestibility, enzyme activities and immune system.     

Fusarium oxysporum f. sp. lycopersici races and their genetic discrimination by molecular markers in West Mediterranean region of Turkey

Fusarium oxysporum f. sp. lycopersici (FOL) races and F. oxysporum f. sp. radicis lycopersici (FORL), the causal agents of root rot and crown rot diseases, respectively, cause serious economic losses in tomato greenhouses where production is intensive in the West Mediterranean region of Turkey. The isolates were collected from West Mediterranean region of Turkey and were characterized by specific primers based on three races (r1, r2, r3), besides pathogenicity tests in in vivo conditions Additionally, a scheme was developed using newly tested ISSR and SRAP markers to a genotyping database and to determine the possible origin of these pathogens. The present study provided new information on these pathogens based on their races and their dominant existence in this region that has not been reported before. Genetic diversity detected in the same races of the pathogen may be associated with difficulties in controlling the pathogen and a possible resistance formation effort exerted by the pathogen to chemicals used in plant protection in tomato greenhouses. Molecular analyses indicated genetic diversity in pathogen isolates identified as r3, r2 and FORL, which may be associated with abiotic stress to which the pathogens were exposed.