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1.
Puglisi CJ Daniel MA Capone DL Elsey GM Prager RH Sefton MA 《Journal of agricultural and food chemistry》2005,53(12):4895-4900
A series of four isomeric 3,9-dihydroxymegastigma-4,6,7-trienes, 8, has been prepared. The (3S,6R,9S) isomer of 8 proved to be identical to an isomer of this compound tentatively identified as an intermediate in the formation of damascenone from an allene triol. Each of the four isomers, when hydrolyzed independently of each other at pH 3.0 and 25 degrees C, produced product mixtures in which the major product was damascenone (1). Contrary to expectation, 3-hydroxydamascone (5) was not observed in any of the hydrolyses. Consequently, the mechanism of formation of damascenone proposed earlier requires modification. In each hydrolysis, the product mixtures showed the presence of a second isomer of 8, produced by epimerization during hydrolysis. Chiral analysis on a Cyclosil B column revealed that this epimerization was occurring at C(3) in each of the hydrolyses. 相似文献
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Development and validation of numerical indexes integrating enzyme activities of soils 总被引:1,自引:0,他引:1
Three numerical indexes, based on soil enzyme activities, which can discriminate between altered and unaltered soils, are presented and validated. Seven enzymes were measured at three different agricultural sites subjected to contamination by municipal and industrial wastes (site 1), intensive cultivation without either crop rotation or organic fertilization (site 2), and irrigation with brackish water (site 3). At each site neighbouring, unaltered soils were sampled and analysed as control soils.The three indexes were developed by means of canonical discriminant analysis (CDA) using data from two sites, while the third site was kept aside as an independent test. The first index is built up using all of the enzyme activities studied; the second, using the most discriminating enzyme activities only; and the third was developed for testing against data in published papers that deal with enzyme measurements in soils under different management regimes.The three indexes were able to discriminate between altered and control soils when applied to the data set from the third site, i.e., that not used for index development. When tested against published data, the third index was usually able to discriminate altered soils from controls by higher index scores. This index was successful in most cases: it was consistently able to classify soils according to their reported degree of alteration. Our results confirm that enzyme activities are suitable indicators of soil alteration and may be usefully integrated to develop soil alteration indexes suitable for monitoring soil status under different conditions. 相似文献
3.
T A Puglisi R W Green C L Hall W K Read R A Green C H Tangner F A Mann H P Hobson 《American journal of veterinary research》1986,47(8):1863-1869
A double-blind study, using metrizamide, iohexol, or Ringer's solution (control) as cisternal myelographic agents, was performed on 25 dogs. Before myelographic examination was done, each dog was subjected to physical, clinical pathologic, and neurologic examinations, as well as examinations by electroencephalography and computerized tomography. These were repeated 24 hours after completion of the myelographic examination. The group of dogs given metrizamide (group II) had a significantly greater occurrence of seizure activity (6 of 10) than did the control dogs (group I; 0 of 5) or dogs given iohexol (group III; 0 of 10; P less than 0.003). In group II, the CSF microprotein concentration was significantly greater 24 hours after myelography was done than were the values in groups I and III (P less than 0.003). Myelograms of the group II dogs (metrizamide) and group III dogs (iohexol) had similar diagnostic qualities. At 24 hours after myelographic examination was done, computerized tomography scan revealed that each dog given metrizamide and iohexol had myelographic contrast material in the brain and cervical spinal cord parenchyma. Seemingly, iohexol has good diagnostic quality, but is less epileptogenic than metrizamide when used in cervical myelographic examinations of dogs. 相似文献
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Muratore G Licciardello F Restuccia C Puglisi ML Giudici P 《Journal of agricultural and food chemistry》2006,54(3):860-863
The influence of concentration and water activity (a(w)) on the formation of 5-hydroxymethyl-2-furancarboxaldehyde (HMF) in thermally treated grape must was evaluated. Must was cryoconcentrated and then heated to study the pure effect of sugar concentration. Moreover, NaCl was added to the must to lower a(w), maintaining the same sugar concentration, with the purpose of evaluating the pure effect of a(w). Finally, the influence of minimal pH changes on the formation of HMF was evaluated by means of a model solution. The results showed that a(w) and sugar concentration are both determinant in the formation of HMF in grape must. Sugar concentration influences the reaction by supplying substrates; low a(w) enhances the formation of HMF by changing the equilibrium in the dehydration step of the reaction. 相似文献
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Janusz A Capone DL Puglisi CJ Perkins MV Elsey GM Sefton MA 《Journal of agricultural and food chemistry》2003,51(26):7759-7763
(E)-1-(2,3,6-Trimethylphenyl)buta-1,3-diene (TPB) was identified as a potent odorant in acid hydrolysates of crude glycoconjugate fractions isolated from grapes and grape vine leaves. TPB was also identified in a Semillon wine, using gas chromatography/mass spectrometry, by co-injection with an authentic sample. TPB had an aroma detection threshold of 40 ng/L in a neutral white wine and the concentration of TPB in four out of five white wines analyzed ranged from 50 to 210 ng/L. 相似文献
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F A Mann H W Boothe M S Amoss C H Tangner T A Puglisi H P Hobson 《Journal of the American Veterinary Medical Association》1989,194(11):1578-1580
Serum testosterone and estradiol 17-beta concentrations, and serum testosterone-to-estradiol ratio were evaluated in 15 dogs (greater than or equal to 5 years old) with perineal hernia (9 sexually intact males and 6 castrated males) and in 9 clinically normal sexually intact male dogs greater than or equal to 5 years old. There was no significant difference in serum testosterone-to-estradiol ratio between sexually intact male dogs with perineal hernia and clinically normal sexually intact male dogs. In castrated dogs with perineal hernia, serum testosterone concentration and testosterone-to-estradiol ratio were significantly (P less than 0.05) lower, compared with those values in sexually intact dogs with perineal hernia and in clinically normal sexually intact male dogs. There was no significant difference in serum estradiol 17-beta concentration among sexually intact male dogs with perineal hernia, castrated dogs with perineal hernia, and clinically normal sexually intact male dogs. Serum testosterone and estradiol 17-beta concentrations in dogs with perineal hernia did not differ from those values in clinically normal male dogs of the same age. Castration cannot be recommended for the treatment of perineal hernia unless a castration-responsive contributing factor such as prostatomegaly is identified, unless the pelvic diaphragm of dogs with perineal hernia has high sensitivity to normal or low serum testosterone and estradiol 17-beta concentrations, or unless there is documentation that other androgens and/or estrogens are involved. 相似文献
9.
The in vitro tests utilized to evaluate sperm quality represent an interesting and important approach to evaluate ejaculated fecundant capacity. In the present work, the oocyte-penetrating ability of sperm from two bulls with low and two with high in vivo fertility rate was investigated. Sperm-quality parameters, such as sperm concentration, total and progressive motility, acrosome and total sperm anomalies, proximal cytoplasmic drops and live : dead sperm ratio were assessed and batches of sperm homologues in these parameters were selected. In expt 1, a sperm : oocyte ratio of 3000 was used and the oocytes were fixed 5, 10, 15 and 18 h post-insemination (hpi). In expt 2, the sperm : oocyte ratio was reduced to 1000 and the eggs were fixed at 8 and 10 hpi. The results, analysed by chi-square test, showed significant differences (p <0.001) among bulls at 15 hpi in expt 1 and 8 hpi in expt 2; nevertheless, no accordance was found between sperm penetration rate and in vivo fertility. At 18 hpi, the low-fertility bulls exceeded the two high-fertility bulls, supporting previous reports that suggest an opposite correlation between in vivo and in vitro fertility rate at low doses of heparin. Furthermore, a more efficient zona binding ability of one high-fertility bull was pointed out in expt 2 after the reduction of sperm : oocyte ratio, as it reached the highest percentage of penetration when compared with all the others. 相似文献
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