Primary sulfates in atmospheric sulfates: estimation by oxygen isotope ratio measurements

The relative amounts of primary and secondary sulfates in atmospheric aerosols and precipitation can be estimated from measurements of the stable oxygen isotope ratios. The oxygen-18 content of sulfates formed in power plant stack gases before emission into the atmosphere is significantly higher than that of sulfates formed from sulfur dioxide after emission. Results show that 20 to 30 percent of the sulfates in rain and snow at Argonne, Illinois, are of primary origin.     

Association of a Geminivirus with a Leaf Curl Disease of Sunn Hemp (Crotalaria juncea) in India

Natural occurrence of a geminivirus causing severe leaf curl disease on sunn hemp (Crotalaria juncea) was recorded in India. The association of a geminivirus with the disease was demonstrated by whitefly transmission tests and polymerase chain reaction (PCR) amplification of DNA fragments of expected sizes with three pairs of degenerate geminivirus primers. The PCR-amplified viral DNA fragments were further characterized by Southern hybridization with a geminivirus probe consisting of the cloned coat protein (CP) gene of Indian tomato leaf curl virus (ITLCV). Restriction fragment length polymorphism analysis of a PCR-amplified CP fragment revealed that the geminivirus from sunn hemp was different than ITLCV.     

Organic zinc and copper supplementation on antioxidant protective mechanism and their correlation with sperm functional characteristics in goats

Trace minerals feeding had significant effects on sperm production and fertility with better absorption and proper utilization within the body for optimum reproductive function. Several studies have shown that more influenced trace elements in the diets of animals are copper (Cu) and zinc (Zn). Bucks showing deficiency of this mineral might affect the quality of semen production which in turn would affect the fertility. This experiment was thus designed to test the effects of organic Cu and Zn supplementation on antioxidants enzyme activities and sperm functional attributes in fresh semen of bucks. Forty bucks (n = 40, Aged 5 months) were assigned to ten groups of four animals in each group, supplemented (for a period of 8 months) with different levels of organic Zn: 20 mg (T2), 40 mg (T3) and 60 mg (T4), organic Cu: 12.5 mg (T5), 25 mg (T6), 37.5 mg (T7) and combined organic Zn and Cu: 20 + 12.5 mg (T8), 40 + 25 mg (T9), 60 + 37.5 mg (T10), respectively, per kg dry matter and no additional mineral diet (control; T1). One hundred and sixty semen samples were collected through electro‐ejaculator and analysed for sperm quantity, quality, acrosome intactness and plasma membrane integrity and correlated with the catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase enzyme activities in seminal plasma. The results indicated organic Cu and zinc supplemented bucks produced more sperm cells, had higher sperm concentrations, maintained higher (p < .01) sperm livability, plasma membrane and acrosome integrities, more motility and velocity. The increased antioxidant enzyme activities, reduced oxidative stress and lowered lipid peroxidation were positively correlated (p < .05) with the sperm functional attributes. In conclusion, organic Cu and Zn supplement to male goats showed protective roles against oxidative damage and maintained better fresh semen characteristics.     

Effect of conservation agriculture on soil organic and inorganic carbon sequestration and lability: A study from a rice–wheat cropping system on a calcareous soil of the eastern Indo-Gangetic Plains

Increasing soil carbon (C) in arable soils is an important strategy to achieve sustainable yields and mitigate climate change. We investigated changes in soil organic and inorganic carbon (SOC and SIC) under conservation agriculture (CA) in a calcareous soil of the eastern Indo-Gangetic Plains of India. The treatments were as follows: conventional-till rice and wheat (CT-CT), CT rice and zero-till wheat (CT-ZT), ZT direct seeded rice (DSR) and CT wheat (ZT-CT), ZTDSR and ZT wheat without crop residue retention (ZT-ZT), ZT-ZT with residue (ZT-ZT+R), and DSR and wheat both on permanent beds with residue (PB-PB+R). The ZT-ZT+R had the highest total SOC in both 0–15 and 15–30 cm soil layers (20% and 40% higher (p < .05) than CT-CT, respectively), whereas total SIC decreased by 11% and 15% in the respective layers under ZT-ZT+R compared with CT-CT. Non-labile SOC was the largest pool, followed by very labile, labile and less labile SOC. The benefits of ZT and residue retention were greatest for very labile SOC, which showed a significant (p < .05) increase (~50%) under ZT-ZT+R compared with CT-CT. The ZT-ZT+R sequestered ~2 Mg ha⁻¹ total SOC in the 0–15 cm soil layer in 6 years, where CT registered significant losses. Thus, the adoption of CA should be recommended in calcareous soils, for C sequestration, and also as a reclamation technique.