黔白1号施用“容大丰”生态肥的效果

“容大丰”高效复合生态肥是最新一代以腐植酸为基质的可溶性叶面肥料，它集腐植酸、氮、磷、钾和中、微量元素为一体，经生化技术生产为膏状体。在合理使用基肥、正确使用“容大丰”高效复合生态肥的前提下，可有效提高单位面积产量，提早采收。为研究“容大丰”高效复合生态肥在生产上的应用效果，笔者于2004年、2005年进行了大白菜应用“容大丰”高效复合生态肥的试验。     

沙棘叶中4，4′-二羟基-2′-甲氧基查耳酮的分离与鉴定

利用硅胶柱和葡聚糖凝胶柱层析，从沙棘叶子中分离并鉴定出黄酮类成分，进一步分离得到4，4′-二羟基-2′-甲氧基查耳酮，并对其结构进行了结构鉴定。     

从甜瓜子叶中提取总DNA

用分子标记方法检测甜瓜杂交种子纯度,如果从真叶中提取DNA,检测周期至少需要15d。为了缩短检测周期,我们分别以甜瓜的成熟干种子、吸胀水后“露白”种子、3d龄刚转绿子叶、6d龄子叶、9d龄子叶和12d龄子叶为材料,进行了提取总DNA的研究。结果表明:除了干种子和吸胀水后“露白”种子外,其他的材料都可以提取到DNA,但是DNA的质量因子叶日龄不同而存在很大的差异。不同日龄的子叶中,以3d龄子叶提取的DNA质量好。子叶6d龄时,提取的DNA质量次于3d龄,少量DNA开始出现降解,以后随着子叶日龄的增加,DNA降解加重。另外,与以真叶为材料提取的DNA样品相比较,子叶DNA样品中的蛋白质等杂质含量高,应增加氯仿抽提纯化次数。     

Long-term effects of TCV116 on left ventricular remodeling and heart function after myocardial infarction in rats

AIM: To investigate the effects of long-term TCV116 on left ventricular remodeling and heart function after myocardial infarction. METHODS: Myocardial infarction (MI) was caused by ligation of the left anterior descending coronary artery in rats. One week after the surgical performance, the surviving rats were randomly assigned to the following treatment protocols: (1) MI rats with no therapy; (2) MI rats treated with TCV116 2 mg/kg per day; (3) Sham-operated control; (4) Sham-operated rats, treated with TCV116 2 mg/kg per day. At 22 weeks, cardiac hemodynamic parameters such as MAP, LVSP, dp/dt_max and LVEDP, and histomorphometric parameters such as LVW/BW and LVCA/BW were measured, mRNA of cardiac genes such as βMHC, BNP, TGF-β₁, collagen I and III were quantified, and survival rates were calculated. RESULTS: Compared with sham-operated rats, MI rats without therapy showed significant increases in histomorphometric parameters as well as in mRAN expressions of cardiac genes (P<0.01); While their hemodynamic parameters were significantly impaired (P<0.01), and survival duration shortened (P<0.05). Compared with MI rats without therapy, MI rats treated with TCV116 showed significant attenuation of mRAN expression of cardiac genes (P<0.01); While their hemodynamic parameters were significantly improved (P<0.05 or P＜0.01), and survival duration extended (P<0.05). CONCLUSION: Treatment with long-term angiotensin II type 1 receptor antagonist may improve left ventricular remodeling and cardiac function after MI in rats.     

实验动物微生物学质量控制及效果观察

以SPF级（无特殊病原体）动物为种源，采取综合防治措施，在屏障设施饲养清洁级实验小鼠和大鼠。经过两年饲养繁育的结果表明：动物微生物学质量符合清洁级动物国家标准，质量控制效果显著。     

SUPEROXIDE DISMUTASE FAMILY GENES IN WATERMELON AND THEIR RESPONSES TO DIFFERENT ABIOTIC STRESSES

• A total of 8 SOD genes from watermelon were identified and bioinformatically analyzed. • The SOD proteins from watermelon and other different plant species can be classified into five groups consistent with their metal cofactors. • ClSOD genes exhibited distinctive tissue-specific and abiotic stress responsive expression patterns. Superoxide dismutase (SOD) is an important enzyme in the antioxidant system of plants and plays a vital role in stress responses by maintaining the dynamic balance of reactive oxygen species (ROS) concentrations. Genome-wide analysis of the SOD gene family in various plant species has been conducted but little is known about this gene family in watermelon (Citrullus lanatus). Here, eight SOD genes were identified in the watermelon genome and are designated ClCSD1-5, ClFSD1-2 and ClMSD according to their metal cofactors. Phylogenetic analysis shows that SOD proteins from various plant species can be classified into five groups and members in the same group possess the same metal cofactor and similar subcellular localizations. Expression analysis of the ClSOD genes indicates that they had tissue-specific expression patterns with high expression in different tissues including the leaves, flowers and fruit. In addition, the expression of ClSOD genes differed appreciably under salinity, drought and abscisic acid (ABA) treatments, indicating that they may be involved in ROS scavenging under different abiotic stresses via an ABA-dependent signaling pathway. These results lay the foundation for elucidating the function of ClSOD genes in stress tolerance and fruit development in watermelon.     

海-陆交互作用与海岸带碳中和农业驱动

海岸带既是生态脆弱区也是低碳农业发展“前哨”。推动和发展碳中和农业符合时代所需,也符合绿色、可持续发展理念。基于此,本文阐述了在海岸带区域发展碳中和农业的相关问题和重要性。首先,本文阐释了海陆交互作用以及海岸带碳中和农业发展理念;然后,分析了海岸带碳中和农业驱动中的碳中和农业生物、人工光合作用与农业驱动以及该理念的工程化应用问题;最后,深入剖析了如何识别海岸带农业核心碳汇,包括原位监测、同位素标记与统计学分析方法,并在此基础上提出了农业碳汇增强技术,为后续海岸带碳中和农业的构建、发展和实践提供参考,同时对促进脆弱的海岸带生态系统的保护和绿色低碳农业的发展具有重要意义。     

Zn2+浓度对鸡腿菇菌丝体生长的影响

将不同浓度的Zn2 加入鸡腿菇JM-08的发酵液中,研究其对菌丝体生长的影响.结果表明,不同浓度的Zn2 对菌丝体生物量、富集Zn2 量以及POD活性均有促进作用,且与对照组达到显著性差异(P＜0.05).当Zn2 浓度为200mg·L-1时,菌丝体生物量和POD活性均达到最大值,Zn2 浓度为300mg·L-1时,菌丝体Zn2 含量达到最大值.     

移栽秧龄对杂交中稻抽穗进程和成熟期的影响

以4个熟期不同的杂交组合为材料进行田阃试验,研究了这些品种在4种秧龄处理下抽穗进程和成熟期的变化.结果表明,在较短秧龄处理下,水稻见穗期有所提前且见穗至完全齐穗所经历的时间较短,抽穗相对集中;在长龄或超长龄处理下,水稻见穗期推迟,见穗至完全齐穗所经历的时间拉长.但生育期不同的品种抽穗进程对秧龄的反应有较大差异.移栽秧龄对同一品种成熟期的影响很小.     

紫苏子油纳米乳的制备及其对小鼠急性高血脂症的影响

通过绘制伪三元相图优选处方,采用滴定法制备紫苏子油纳米乳,用透射电子显微镜(TEM)、激光粒度测定仪分别考察其形态和粒径,通过恒温加速试验考察其稳定性,并研究其对急性小鼠高血脂症的影响。结果显示,以小麦胚芽油为油相,聚氧乙烯氢化蓖麻油(RH40)为表面活性剂制备的紫苏子油纳米乳外观澄明,有淡蓝色乳光;电镜下呈圆球形,平均粒径为58 nm,高速离心、高温下无絮凝或药物析出。与紫苏子油软胶囊对抗急性小鼠高血脂症的药效比较,紫苏子油纳米乳有更强的抗高血脂功效。结果表明,制备的紫苏子油纳米乳是一种质量稳定、高效的抗高血脂乳剂。