Cucumber Mosaic Virus, or Infectious Chlorosis, was found in alarming proportions in all the districts of Tamil Nadu on the ‘Robusta’ (Dwarf Cavendish) and ‘Poovan’ (Mysore) cultivais. The disease was transmitted only through suckers from the diseased gardens. Among the genotypes screened, the clones with ABB genomes showed complete field resistance. 相似文献
1. The effect of probiotic supplementation on egg production, on serum and yolk cholesterol and on egg shell thickness in 24 White Leghorn layers was studied from 28–38 weeks of age.
2. In 3 treatments the diet was supplemented with 0, 100 and 150 mg probiotic/kg food.
3. In the 100 mg probiotic group, egg production improved by 5%, and shell thickness improved slightly, with fewer thin‐shelled eggs than in the control (8.6% compared to 18.6%).
4. The initial serum cholesterol concentration of 170.2 mg/dl in control birds remained similar throughout the 10‐week experimental period, whereas in the 150 mg group the initial value of 176.5 mg/dl decreased to 114.3 mg by week 10.
5. Yolk cholesterol concentration was 14.69 mg in the control group and 11.28 and 11.37 mg/g in the 100 and 150 mg probiotic groups respectively. Overall mean total egg cholesterol was thus reduced by probiotic supplementation. 相似文献
Our previous study evidenced that the 3D CORAGRAF loaded with PLGA microsphere constitutes PDGF-BB can support cell attachment and proliferation and can induce an osteogenic commitment of mesenchymal stromal cells in the in vitro condition. However, how this construct can perform in pathophysiological conditions in terms of repairing critical bone defects is yet to be understood. A study was therefore conducted to investigate the regeneration potential of calvaria critical-size defects using CORAGRAF + PLGA with PDGF-BB + mesenchymal stromal cells (MSCs) in a rat model. A 5 mm critical bone defect was created on calvaria of 40 male Sprague-Dawley rats. CORAGRAF incorporated either with or without PDGF-BB and seeded with rat bone-marrow-derived MSCs was implanted at the defect region. The bone regeneration potential of implanted constructs was assessed using micro-CT imaging and histological staining in weeks 4 and 8. The micro-CT images indicated a significant closure of defects in the cranial bone of the rats treated with 3D CORAGRAF + PLGA with PDGF-BB + MSCs on week 4 and 8 post-implantation. This finding, further supported with the histology outcome where the rat cranial defect treated with CORAGRAF + PLGA with PDGF-BB + MSCs indicated neo-bony ingrowth with organized and mature bone-like morphology as compared with other groups. The previous in vitro results substantiated with our pre-clinical findings demonstrate that the combination of CORAGRAF + PLGA with PDGF-BB + MSCs could be an ideal construct to support bone regeneration in critical bone defects. Hence, this construct can be further investigated for its safety and efficacy in large animal models, or it can be skipped to human trial prior for commercialization. 相似文献
The diagnosis and treatment of double intestinal intussusception in 3 pups with persistent vomiting, diarrhea, dehydration, anemia, leucocytosis, and electrolyte imbalance are described. Ultrasonography confirmed intussusception and laparotomy revealed double intussusceptions. Intussusceptions were corrected by manual reduction in 1 pup and intestinal resection and anastomosis in 2 pups. Two pups survived and 1 pup died on the 4th day after surgery. 相似文献
ABSTRACT Rice-Wheat rotation is the dominant land use in the state of Punjab, resulting in over exploitation of ground water resources. Thus, it is necessary to evaluate other land uses that requires less water and are sustainable. The present investigation was planned with four land uses viz., fallow (FLU), rice-wheat (ALU), grasses (GLU) and pear (PLU) with respect to their effect on soil organic carbon and soil physical characteristics in surface and subsurface depth in district Ludhiana, Punjab. The soil organic carbon (SOC) content was higher by 10, 30.9 and 24.9% under rice-wheat, grasses, and pear than that under fallow. The grasses showed higher soil moisture characteristics curve (SMCC) and lower bulk density (Db) than that under rice-wheat. The larger mean weight diameter (MWD) were observed under pear, grasses and fallow than that under rice-wheat by 0.21, 0.51 and 0.41 mm, respectively. The saturated hydraulic conductivity (Ks) was higher in magnitude by 56.1, 55.4 and 28%, respectively under PLU, GLU and FLU over ALU. Of the evaluated land uses, pear and grasses proved to be more sustainable by retaining more moisture, maintaining better soil physical characteristics and SOC under semiarid irrigated conditions in the state on long term gradual response. 相似文献
Grain legumes, the important constituents of sustainability‐based cropping systems and energy‐limited vegetarian diets have long been the subject of scientific research. Tremendous technological strides were made in the so‐called orphan crops, in terms of both varietal improvement and generation of basic information. Despite recalcitrancy and high genotype dependency, in vitro culture techniques such as organogenesis, in vitro mutagenesis, embryo rescue and in vitro gene transfer have been deployed for improvement of several grain legumes and these played an important role in introgression of desirable genes from related and distant species and creation of additional genetic variability. Stable and reproducible regeneration protocols resulted in the development of genetically modified chickpea, pigeon pea, cowpea, mungbean, etc., while embryo rescue was deployed successfully for recovery of interspecific recombinants, a few of them exploited for the development of commercial cultivars. Nevertheless, doubled haploidy witnessed limited success and protoplast regeneration and in vitro mutagenesis remained of academic interest. The present review focuses on the progress, achievements, constraints and perspectives of using in vitro technology in grain legume improvement. 相似文献
The quantity of water available for irrigation is getting scarce in many countries and it assumes great importance for assured crop production, especially in view of the erratic behavior of the monsoon. Thus, there is a pressing need to improve the water efficiency of irrigation systems. One-way of improving the efficiency of the irrigation system is reusing the return flow from the irrigation system. This task requires quantification of return flow, which still remains as a grey area in irrigation water management. The estimation of return flow from the irrigation system is usually obtained using thumb rules depending upon the site-specific conditions like command area conditions and soil properties. In this paper, a hierarchical modeling technique, namely, regression tree is developed for return flow estimation. Regression tree is built through binary recursive partitioning. The effective rainfall, inflow, consumptive water demand, and percolation loss are taken as predictor variables and return flow is treated as the target variable. The applicability of the hierarchical model is demonstrated through a case study of Periyar-Vaigai Irrigation System in Tamil Nadu, India. The model performance shows a good match between the simulated and the field measured return flow values. Results of statistical analysis indicated that the correlation coefficients are high for both single as well as double crop seasons. 相似文献
Abstract Strawberry shoot meristem cultures have been used for producing virus-free strawberry plants. Plantlet regneration from leaf mesophyll protoplasts, and Agrobacterium tumefaciens mediated genetic transformation, mark the beginning of strawberry improvement with biotechnologies. The use of biotechnological tools has a greater potential in producing strawberry plants with traits such as early maturation, frost and disease resistance, mite and nematode resistance, high yield, better quality, suitability for mechanical harvesting, shipping ability, ellgaic acid content, aroma compounds and cost-effective micropropagation. Gene identification and mapping with RFLPs or RAPDs would be desirable. Transgenic strawberries should be tested for their quality before releasing to the consumers. 相似文献
A new leaf spot disease of banana(Musa sp.) cv. ‘Rasthali’ (syn. Silk) was observed in Tamil Nadu, in 1987. The causal organism was isolated in pure culture and
identified asRhizoctonia solarii Kühn. The teleomorphic stage,Thanatephorus cucumeris (Frank) Donk, has also been induced artificially. This is the first record of this pathogen causing leaf spot disease onMusa sp. The pathogenicity of basidiospores on several other important crops was also tested. 相似文献