Note: New records of flea beetle (Coleoptera: Chrysomelidae) from Turkey

Five species of flea beetles (Coleoptera: Chrysomelidae) were recorded for the first time in Turkey:Dibolia numidica Doguet,Longitarsus corynthius corynthius (Reiche et Saulcy),L. onosomae (Peyerimhoff),L. pulmonariae Weise andPhyllotreta ganglbaueri Heikertinger. The zoogeographical distribution of the species was reviewed. Among the species,L. onosmae andD. numidica have a limited distribution area in North Africa, and therefore their presence in Turkey is interesting. http://www.phytoparasitica.org posting July 14, 2004.     

Gamma radiation sensitivity of the eggs, larvae and pupae of Indian meal moth Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae)

BACKGROUND: This study focused on determining the minimal effective gamma radiation dose that prevents commodity damage caused by the Indian meal moth Plodia interpunctella (Hübner). The study was also designed to assess the effect of releasing either partially sterilized males alone or both partially sterilized males and females on the reproductive potential of P. interpunctella populations. RESULTS: The dose of radiation required to prevent larval emergence from irradiated eggs was 350 Gy, and the same dose was also required to prevent adult emergence from mature larvae. A dose of 300 Gy was not able to prevent adult emergence from irradiated pupae. The dose at which 100% sterility was achieved in treated females mated to treated males was 300 Gy for the parental generation of irradiated pupae. Fertility of the parental males from irradiated pupae was 48.17% at 300 Gy in treated males crossed with untreated females, but male progeny of irradiated male parents had a residual fertility of 11.06% at the same dose. CONCLUSION: F(1) males from irradiated pupae were more sterile than parental males. To prevent larval emergence from irradiated eggs, a dose of 350 Gy is required. The same dose is required to prevent the larvae from reaching the adult stage.     

Influences of host age, sex ratio, population density, and photoperiod on parasitism by Trichogramma evanescens Westw. (Hym., Trichogrammatidae)

Moth species Ephestia kuehniella and Sitotroga cerealella are serious pests in cereal-based food processing facilities and stores in Turkey. Control of these pests is undertaken by regular space treatment of infested areas with pesticides. An alternative control method could be the release of parasitic wasps of the genus Trichogramma. In laboratory tests, we use T. evanescens as a parasitic wasp reared on the eggs of Ephestia kuehniella. Adult wasps emerging from the host were maintained in glass tubes at 27±1° C, 60–70±5% r.h and L14:D10 and fed on honey solution. Adults of T. evanescens in vials without food enclosed from host eggs and all died within 1.8days; in comparison, in vials with honey, live adults were evident for 15.2days. Fresh (6–48h) and old (72–96h) host eggs were offered to T. evanescens and fresh eggs were more accepted than old. Host acceptance of females with males in vials was better than without males. The number of females in the vials also influenced the parasitization rate and single female parasitized more eggs. Adults living in light regime (L14:D10 and L6:D18) parasitized more eggs than in total darkness. Results are discussed with relation to enhancing parasitoid effectiveness in biological control in processing facilities and mills.     

Effects of gamma radiation on life stages of the Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)

We report the results of experiments conducted with Ephestia kuehniella Zeller to determine the effects of gamma radiation on life stages. Eggs, larvae, pupae and adults were irradiated with increasing doses of gamma radiation (seven dose levels between 50 and 400 Gy for eggs and larvae, six dose levels between 50 and 350 Gy for pupae and four dose levels between 250 and 550 Gy for adults). All the experiments were conducted in a growth chamber maintained at 27 ± 1°C, 65 ± 5% r.h., and alternating 14 h light:10 h dark cycles. The number of adults that developed from irradiated eggs and larvae was lower than the untreated control. Doses of 200 Gy and above prevented adult emergence from irradiated eggs. Although a dose of 200 Gy was enough to prevent adult emergence from young larvae, 250 Gy should be used to prevent adult emergence completely from last instar larvae. Delayed developmental periods were observed for the treated eggs and larvae. Fecundity and egg hatchability were decreased depending on the doses applied. Decreased fecundity and egg hatchability were more prevalent when both the male and female pupae were treated compared to the treatment of female pupae only. There was no significant decrease in the fecundity of irradiated adults except 550 Gy, and no eggs hatched at doses of 300 Gy and above.     

Boron stress,oxidative damage and antioxidant protection in potato cultivars (Solanum tuberosum L.)

Six potato cultivars grown in Turkey in boron-prone areas and differing in their tolerance towards high boron were studied to reveal whether boron causes oxidative stress. To assess stress level, chlorophyll fluorescence and growth parameters were measured. Oxidative damage was assessed as malondialdehyde level, and antioxidant protection was evaluated as ascorbate (AA), dehydroascorbate, reduced glutathione (GSH) and oxidized glutathione amounts and superoxide dismutase, catalase, ascorbate peroxidase (APX) and glutathione reductase (GR) activities. High boron stress affected photosynthesis negatively in a threshold-dependent manner and inhibited growth. No pronounced changes in oxidation of lipids occurred in any cultivar. Activation of APX suggested the involvement of an ascorbic acid–reduced glutathione cycle in the protection against oxidative stress caused by high boron. Efficient work of this antioxidant system was probably hindered by boron complexation with NAD(P)⁺/NAD(P)H and resulted in the inhibition of GR and a decrease in AA and GSH. Hence, oxidative stress associated with high boron is a secondary component of boron toxicity which arises from metabolic changes caused by boron interference with major metabolites. Potato cultivars tolerate excess boron stress well and show damage only in very high boron concentrations. The potato cvs best suited for high boron soils/breeding purposes are cvs Van Gogh and Agria.

Abbreviations: AA: ascorbic acid; APX: ascorbate peroxidase; CAT: catalase; DHA: dehydroascorbic acid; DHAR: dehydroascorbate reductase; DTNB: 5; 5′-dithiobis-2-nitrobenzoic acid; DTT: dithiotreitol; F_v/F_m: photosynthetic efficiency at the dark-adapted state; GR: glutathione reductase; GSH: reduced glutathione; GSSG: oxidized glutathione; MDA: malondialdehyde; ROS: reactive oxygen species; SOD: superoxide dismutase; TCA: trichloroacetic acid     

Gamma radiation sensitivity of larvae and adults of the red flour beetle, Tribolium castaneum Herbst

In this study, the susceptibility of larvae and adults of Tribolium castaneum (Herbst) to gamma radiation was investigated in the laboratory in Turkey. Gamma radiation was applied at 6 dose levels between 20 and 200Gy to 13–15day-old adults and at 5 dose levels between 20 and 180Gy to 18–20day-old larvae of T. castaneum. All experiments were performed in growth chambers maintained at 27±1°C and 70±5% r.h. The larvae proved to be the more susceptible stage, survival to the adult stage being prevented by exposure of the eggs to 100Gy; the adult stage was less susceptible. LD₅₀ and LD₉₉ values were determined as 19,75 and 42,97Gy for larvae and 33,21 and 64,50Gy for adult stage, respectively. It was concluded that 100Gy is the effective dose for both larval and adult stages.     

Melanoma Inhibition by Anthocyanins Is Associated with the Reduction of Oxidative Stress Biomarkers and Changes in Mitochondrial Membrane Potential

Anthocyanins are water soluble pigments which have been proved to exhibit health benefits. Several studies have investigated their effects on several types of cancer, but little attention has been given to melanoma. The phytochemical content of nine different berry samples was assessed by liquid chromatography followed by electrospray ionization mass spectrometry (LC-ESI⁺-MS). Twenty-six anthocyanins were identified, after a previous C₁₈ Sep-pak clean-up procedure. Chokeberry and red grape anthocyanins rich extracts (C-ARE and RG-ARE) were selected to be tested on normal and melanoma cell lines, due to their different chemical pattern. C-ARE composition consists of cyanidin aglycone glycosylated with different sugars; while RG-ARE contains glucosylated derivatives of five different aglycones. Both C-ARE and RG-ARE anthocyanins reduced proliferation, increased oxidative stress biomarkers and diminished mitochondrial membrane potential in melanoma cells, having no negative influence on normal cells. A synergistic response may be attributed to the five different aglycones present in RG-ARE, which proved to exert greater effects on melanoma cells than the mixture of cyanidin derivatives with different sugars (C-ARE). In conclusion, C-ARE and RG-ARE anthocyanins may inhibit melanoma cell proliferation and increase the level of oxidative stress, with opposite effect on normal cells. Therefore, anthocyanins might be recommended as active ingredients for cosmetic and nutraceutical industry.

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Graphical Abstract ?

     

Influences of host age,sex ratio,population density,and photoperiod on parasitism by <Emphasis Type="Italic">Trichogramma evanescens</Emphasis> Westw. (Hym., Trichogrammatidae)

Moth species Ephestia kuehniella and Sitotroga cerealella are serious pests in cereal-based food processing facilities and stores in Turkey. Control of these pests is undertaken by regular space treatment of infested areas with pesticides. An alternative control method could be the release of parasitic wasps of the genus Trichogramma. In laboratory tests, we use T. evanescens as a parasitic wasp reared on the eggs of Ephestia kuehniella. Adult wasps emerging from the host were maintained in glass tubes at 27±1° C, 60–70±5% r.h and L14:D10 and fed on honey solution. Adults of T. evanescens in vials without food enclosed from host eggs and all died within 1.8days; in comparison, in vials with honey, live adults were evident for 15.2days. Fresh (6–48h) and old (72–96h) host eggs were offered to T. evanescens and fresh eggs were more accepted than old. Host acceptance of females with males in vials was better than without males. The number of females in the vials also influenced the parasitization rate and single female parasitized more eggs. Adults living in light regime (L14:D10 and L6:D18) parasitized more eggs than in total darkness. Results are discussed with relation to enhancing parasitoid effectiveness in biological control in processing facilities and mills.