全文获取类型
收费全文 | 12060篇 |
免费 | 663篇 |
国内免费 | 1147篇 |
专业分类
林业 | 980篇 |
农学 | 1082篇 |
基础科学 | 612篇 |
1465篇 | |
综合类 | 5022篇 |
农作物 | 813篇 |
水产渔业 | 570篇 |
畜牧兽医 | 1801篇 |
园艺 | 893篇 |
植物保护 | 632篇 |
出版年
2024年 | 86篇 |
2023年 | 264篇 |
2022年 | 503篇 |
2021年 | 614篇 |
2020年 | 499篇 |
2019年 | 503篇 |
2018年 | 374篇 |
2017年 | 568篇 |
2016年 | 438篇 |
2015年 | 586篇 |
2014年 | 614篇 |
2013年 | 698篇 |
2012年 | 1018篇 |
2011年 | 1070篇 |
2010年 | 978篇 |
2009年 | 784篇 |
2008年 | 840篇 |
2007年 | 761篇 |
2006年 | 658篇 |
2005年 | 485篇 |
2004年 | 301篇 |
2003年 | 190篇 |
2002年 | 236篇 |
2001年 | 156篇 |
2000年 | 141篇 |
1999年 | 104篇 |
1998年 | 60篇 |
1997年 | 44篇 |
1996年 | 51篇 |
1995年 | 40篇 |
1994年 | 28篇 |
1993年 | 32篇 |
1992年 | 33篇 |
1991年 | 17篇 |
1990年 | 17篇 |
1989年 | 16篇 |
1988年 | 10篇 |
1987年 | 10篇 |
1986年 | 4篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1981年 | 5篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1976年 | 2篇 |
1974年 | 2篇 |
1971年 | 2篇 |
1962年 | 2篇 |
1956年 | 4篇 |
1955年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
1.
本试验是对测定饲料中钙含量的3种方法,即双波长吸光光度法、MSA—DBM显色法和高锰酸钾法的比较试验。综合本实验结果,建议饲料生产中采用MSA—DBM显色法,可以简便快速的得到可靠结果。 相似文献
2.
3.
V Danielová D Málková J Minár B Rehse-Küpper Z Hájková J Halgos L Jedlicka 《Folia parasitologica》1978,25(2):187-190
In the years 1973 and 1975 mosquitoes and some other Diptera (Tabanidae, Simuliidae, Hippoboscidae) were tested for virus. 13,924 mosquitoes, 75 horseflies and 60 blackflies were processed in 1973. Five strains of Tahyna virus were isolated from mosquito species Aedes vexans. 3,378 mosquitoes and 12 sheep keds were tested for virus in 1975. Twelve strains of Calovo virus were isolated from Anopheles maculipennis and one strain of Tahyna virus was obtained from Aedes vexans mosquitoes. 相似文献
4.
5.
AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer. 相似文献
6.
奶牛“两病”是指奶牛的结核病和布鲁氏菌病,是世界动物卫生组织(OIE)规定强制报告的两种疫病,二病都属人畜共患病。人的牛型结核感染主要是通过饮用生奶引起,尤其是儿童常因喝生牛奶被感染而患肠结核杆菌病;饮用生奶或制品也易感染布鲁氏菌病。奶牛“两病”严重威胁着人民的身体健 相似文献
7.
红豆草壳二孢叶斑黑茎病(Ascochyta onobrychidis)是红豆草(Onobrychis viciaefolia)最重要的病害之一,在分离此病菌的同时得到一种对此病菌具有较强抑制作用的微生物,为评价此拮抗菌的开发利用前景,本研究采用形态学、分子生物学和生物化学方法鉴定了其分类地位,并测定了其对红豆草壳二孢的抑菌效果。通过构建系统发育树并结合其形态特征和生理生化特性,将该拮抗菌鉴定为微白黄链霉菌(Streptomyces albidoflavu),在高氏一号培养基上菌落为黄白色,可产生黄白色水溶性色素,孢子丝钩状,顶端螺旋形;在平板对峙试验中该菌的菌落对红豆草壳二孢菌落的相对抑制率达74.33%,同时该菌发酵液对红豆草壳二孢菌丝生长和孢子萌发的抑制率分别为80.95%和93.87%。该菌具有生物防治红豆草壳二孢的开发和利用价值。 相似文献
8.
9.
Hyun-Soo Kim Jun-Geon Je Hyesuck An Kyunghwa Baek Jeong Min Lee Mi-Jin Yim Seok-Chun Ko Ji-Yul Kim Gun-Woo Oh Min-Cheol Kang Young Min Ham You-Jin Jeon Dae-Sung Lee 《Marine drugs》2022,20(8)
Ecklonia maxima is a brown seaweed, which is abundantly distributed in South Africa. This study investigated an efficient approach using high-performance centrifugal partition chromatography (HPCPC), which has been successfully developed for the isolation and purification of phlorotannins, eckmaxol, and dieckol from the ethyl acetate fraction of E. maxima (EEM). We evaluated EEM for its inhibitory effect against lipopolysaccharide (LPS)-induced inflammatory responses in zebrafish embryos. The separation of eckmaxol and dieckol from samples of EEM using HPCPC was found to be of high purity and yield under an optimal solvent system composed of n-hexane:ethyl acetate:methanol:water (2:7:3:7, v/v/v/v). To evaluate the anti-inflammatory efficacy of EEM containing active compounds, zebrafish embryos exposed to LPS were compared with and without EEM treatment for nitric oxide (NO) production, reactive oxygen species (ROS) generation, and cell death two days after fertilization. These evaluations indicate that EEM alleviated inflammation by inhibiting cell death, ROS, and NO generation induced by LPS treatment. According to these results, eckmaxol and dieckol isolated from brown seaweed E. maxima could be considered effective anti-inflammatory agents as pharmaceutical and functional food ingredients. 相似文献
10.
高通量测序发现河南开封、中牟西瓜病毒病由多种病毒复合侵染导致 总被引:2,自引:2,他引:0
调查发现河南省开封市祥符区和郑州市中牟县西瓜田病毒病严重发生,症状表现为叶片黄化、蕨叶、皱缩、卷曲,枝梢翘起,果实变小和瓜纹不清。为明确其病毒种类,通过小RNA测序及生物信息学分析发现采集的西瓜样本中存在8种病毒。其中包括5种已知病毒:小西葫芦黄花叶病毒(Zucchini yellow mosaic virus, ZYMV)、甜瓜蚜传黄化病毒(Melon aphid-borne yellows virus, MABYV)、西瓜花叶病毒(Watermelon mosaic virus, WMV)、黄瓜绿斑驳花叶病毒(Cucumber green mottle mo-saic virus, CGMMV)和西瓜隐潜病毒(Citrullus lanatus cryptic virus, CiLCV);3种为新检出的RNA病毒:西瓜病毒A(Watermelon virus A, WVA)、西瓜皱叶相关病毒1号(Watermelon crinkle leaf-associated virus 1, WCLaV-1)和西瓜皱叶相关病毒2号(Watermelon crinkle leaf-associ... 相似文献