O型口蹄疫疫苗免疫牛抗体消长动态的LPB-ELISA检测

分别按一次免疫、首免后第15d加强免疫和首免后第60d加强免疫程序，对3组试验牛（每组牛20头）用O型口蹄疫疫苗进行了免疫。免疫后不同时间点用液相阻断ELISA（LPB-ELISA）测定了免疫牛O型口蹄疫抗体效价。结果，首免后第15d加强免疫牛和第60d加强免疫牛抗体水平较高，50％保护牛所占比例分别为56．3％和63．1％，完全受保护的牛所占比例分别为34．3％和29．5％。第60d加强免疫牛的保护率要高于第15d加强免疫牛和一次免疫牛的保护率。结果表明，首免后第60d加强免疫是最理想的免疫程序。     

非线性、分形与期锌市场价格

从非线性角度研究期锌市场，运用R/S分析法对期锌市场进行分析，发现期锌市场的Hurst指数为0.6183,其价格收益率序列是分维时间序列，具有分形结构，维数为1.6174。期锌市场的平均周期长度为176天，具有强持续性和相关性。关联系数为19.47%，远远大于国外相关市场。在风险方面，期锌市场的风险比期铜市场的要小，而比股票市场的风险要大。     

An Improved Method of the Transient Waveform Analysis in Power Systems

The angular frequency and decaying constant of each copmonect for the transient waveform in power systems were obtained by using the discrete Fourier transform; the amplitude and phase angle were calculated in the linear curve fitting method. And all of the parameters obtained above were treated in an unified optimization by using the curve fitting optimization of the least square method of general nonlinear funcrion so as to get the results of waveform analysis. Two examples are included.     

简单屋顶绿化的滞蓄特性

设计了模拟降雨与数据采集系统,研究了短时强降雨下简单屋顶绿化基质(配比：陶粒57.0%、草炭41.5%、保水剂1.5%)的降雨产流过程,基于37次模拟降雨实验,归纳了降雨产流的一般过程、规律和特性,分析了降雨强度、基质厚度和基质初始含水率对简单屋顶绿化滞流蓄水特性的影响。结果表明：基质厚度和基质初始含水率对简单屋顶绿化的蓄水特性有显著影响,基质初始含水率越低、基质厚度越厚时蓄水特性越佳。建立了产流时间数学模型,简单屋顶绿化降雨产流时间与降雨强度呈负相关,同时也受初始含水量和基质厚度影响。随着干湿循环次数增加,基质的厚度总体呈现下降趋势,储水性能逐渐下降,延迟产流时间逐渐缩短。     

An ecosystem-based assessment of the Korean blue crab trammel net fishery in the Yellow Sea and management implications

Landings in the blue crab, Portunus trituberculatus, fishery in Korean waters of the Yellow Sea have declined substantially from 11,000 t in the 1980s to 2,300 t in 2004. Blue crab habitat quality in the Yellow Sea has been degraded by anthropogenic activities including sand mining, land reclamation, and coastal pollution. Various traditional management measures have been implemented, including closed seasons during spawning and size limits, but these measures alone have been unsuccessful to conserve blue crab stocks. Consequently, a total allowable catch and a stock-rebuilding program using an ecosystem-based management approach were implemented in 2003 and 2006, respectively to rebuild blue crab stocks and restore habitats. This program involved assessment of both blue crab stock status and trammel-net fishery impacts at an ecosystem-level using an ecosystem-based fisheries assessment method ( [Zhang et al., 2009] and [Zhang et al., 2010]), which considered fishery data from catch and effort time-series, crab population biology, and ecosystem characteristics, including habitats and environmental conditions. Recent (2008) management status indices have shown significant positive change compared to conditions in 2000 with respect to sustainability of the stock and fishery and with regards to biodiversity and ecosystem habitat quality.     

扇贝多肽对氧化所致胸腺细胞凋亡的影响

观察扇贝多肽(Polypeptide from Chlamys ferreri)(PCF)对实验性氧化所致小鼠胸腺细胞凋亡的影响。采用台盼蓝(Trypan Blue)活细胞拒染法在普通光学显微镜下观察细胞的存活率；以四甲基氮唑盐(MTT)法测定细胞的增殖活性；以生化检测(DNA ladder)法观察PCF对细胞DNA片断化的影响；用透射电镜观察细胞的超微形态结构变化。台盼蓝染色法证实PCF能增强细胞的活性并与PCF浓度成正比；MTT法表明PCF不仅能保护细胞免受损伤，而且还能促进细胞的增殖，并有PCF依赖性；生化检测显示PCF能减少细胞凋亡的DNA片断化数目；细胞的超微结构还表明PCF能维持细胞结构的完整性。PCF可明显抑制实验性氧化损伤小鼠胸腺细胞的凋亡并有药物依赖性，而且明显优于维生素C。     

秋季济南地区鱼类群落结构及其与环境因子的关系

于2014年和2015年秋季对济南地区23个采样点的鱼类群落结构和环境因子特征进行了野外调查。结果表明:两年调查中采集到鱼类分别为5目25种和6目28种,各采样点鱼类个体数量平均值为45.61尾和66.48尾,香农威纳指数分别为1.45和1.81,均匀度指数分别为0.72和0.74。典范对应分析表明,2014年影响鱼类群落结构的主要环境因子是水温和总氮;2015年影响鱼类群落结构的主要环境因子是总磷和总氮。     

西瓜、甜瓜果实品质及调控研究进展

西瓜甜瓜深受消费者喜爱,但是近年来品质呈现下降的趋势.西瓜甜瓜的品质包括外观品质、营养品质和风味品质,而品质的形成又受品种、土壤环境、肥料供应、水分供应、栽培方式、生长调节剂以及采收与贮运等因素的影响,所以要针对以上因素,从改进育种手段和栽培技术2大方面来改良和提高品质.     

哈尔滨市农业生态系统能值分析

应用能值分析理论与方法对哈尔滨市农业生态系统2000—2009年的能值变化及可持续性动态进行了分析。结果表明:哈尔滨市农业生态系统总能值用量呈不断波动趋势,农业生态系统维持对不可更新工业辅助能的依赖性较大,环境负载率居国内较高,可持续性发展压力较大。未来农业生态系统管理要大力发展节水灌溉农业;应积极引进先进技术,增加劳动力的智力资源能值投入,调整工业辅助能投入,提高能值受益率;并探索建立多层次、高功能的生态农业模式。