Stillbirth/perinatal weak calf syndrome: preliminary pathological, microbiological and biochemical findings.

Microbiological, biochemical and pathological data collected from 293 calves which were either stillborn, or born alive and either failed to breathe or failed to breathe for more than about 10 minutes are presented. No bacteria were recovered from 96 of the calves (32.7 per cent), and bacteria which were considered significant were isolated from only four (1.4 per cent). Evidence of leptospiral infection was found in 75 calves (25.5 per cent). Of 64 calves examined for bovine virus diarrhoea (BVD) and infectious bovine rhinotracheitis (IBR) antigens, two were positive for BVD virus and none for IBR virus. The mean (+/- sd) liver vitamin E and kidney selenium concentrations, determined in 148 of the calves, were 2.0 +/- 0.76 micrograms/g wet matter and 0.47 +/- 0.17 micrograms/g wet matter, respectively. The thyroid iodine concentration in 15 of 71 calves (21 per cent) was less than 300 micrograms/g wet matter and the mean (+/- sd) thyroid weight of 266 of the calves was 18.5 +/- 11.6 g. Evidence of severe trauma was found in 19 of the calves (6.5 per cent). Histological findings included thyroid epithelial hyperplasia, hepatic haemosiderosis, erythrophagocytosis in the spleen, perivascular haemorrhage in brain and adrenal glands, and accumulation of leucocytes in blood vessels.     

Triblock copolymer syntheses of mesoporous silica with periodic 50 to 300 angstrom pores

Use of amphiphilic triblock copolymers to direct the organization of polymerizing silica species has resulted in the preparation of well-ordered hexagonal mesoporous silica structures (SBA-15) with uniform pore sizes up to approximately 300 angstroms. The SBA-15 materials are synthesized in acidic media to produce highly ordered, two-dimensional hexagonal (space group p6mm) silica-block copolymer mesophases. Calcination at 500 degrees C gives porous structures with unusually large interlattice d spacings of 74.5 to 320 angstroms between the (100) planes, pore sizes from 46 to 300 angstroms, pore volume fractions up to 0.85, and silica wall thicknesses of 31 to 64 angstroms. SBA-15 can be readily prepared over a wide range of uniform pore sizes and pore wall thicknesses at low temperature (35 degrees to 80 degrees C), using a variety of poly(alkylene oxide) triblock copolymers and by the addition of cosolvent organic molecules. The block copolymer species can be recovered for reuse by solvent extraction with ethanol or removed by heating at 140 degrees C for 3 hours, in both cases, yielding a product that is thermally stable in boiling water.     

肉鸡细菌性软骨坏死的病因及防制

爱尔兰对肉仔鸡软骨病的病因及控制措施进行了系统研究。经过纵向的微生物学和组织学调查发现 ,伴随骨髓炎出现的股骨上端和 /或胫跗关节的细菌性软骨坏死 ( BCF/T)是商品肉仔鸡软骨病的主要原因之一。1　细菌性软骨坏死的症状及诊断患细菌性软骨坏死的跛行肉仔鸡 ,常用翅膀着地 ,支撑发病一侧的身体。发病鸡不能到料槽和饮水器旁采食饮水 ,迅速脱水、失重。剖检时 ,应小心地从髋关节分离出股骨 ,避免外伤 ,防止与病变混淆。肉眼检查时 ,当观察到骺板部位有轻微肿胀 ,就可怀疑一侧或两侧股骨上端或胫跗关节有细菌性软骨坏死。肉眼可以观察…     

Effect of partial replacement of gum arabic with carbohydrates on its microencapsulation properties.

Gum arabic solutions (10% w/v) were emulsified with soy oil at oil/gum ratios of 0.25-5.0. At oil/gum ratios <1.0, it was established that gum arabic could be partially replaced with a nonsurfactant carbohydrate. To assess different carbohydrates as replacers for gum arabic, emulsions and spray-dried emulsions of soy oil and mixed solutions (10% w/v) of gum arabic and a range of carbohydrate wall materials (oil/gum = 0.5) were prepared and analyzed. Maize starch and glucose were ineffective as partial replacers of gum arabic, but maltodextrins of various dextrose equivalence values (5.5-38) successfully replaced 50% of the gum arabic. The microencapsulation efficiency of the gum arabic/maltodextrin stabilized powders was further increased by increasing total solids of the feed to the dryer and by increasing the atomizer nozzle diameter.     

Comparison of Staphylococcus aureus recovered from personnel in a poultry hatchery and in broiler parent farms with those isolated from skeletal disease in broilers.

Personnel from one broiler hatchery, and workers on 18 separate broiler parent farms which supply the hatchery, were tested for hand and nasal carriage of Staphylococcus aureus. In both locations, nasal carriage of S. aureus was more common than hand carriage. A total of 63 S. aureus strains were characterised by biotyping, protein A analysis and pulsed field gel electrophoresis (PFGE) typing. Of these, 36 were recovered from broiler hatchery personnel, 14 from broiler parent farm personnel and 13 from cases of skeletal disease in commercial broilers. Biotyping and protein A analysis indicated that none of the strains recovered from hatchery personnel were of the poultry biotype, but that two strains recovered from the hands of two broiler parent farm personnel could be grouped together with 12/13 of strains recovered from skeletal disease in broilers, as poultry biotypes. PFGE-typing could not distinguish 9/13 strains recovered from skeletal disease in broilers and one of the strains from the broiler parent farm personnel from isolate 24 (I. 24), which is the predominant S. aureus strain type associated with clinical disease in N. Ireland broiler flocks. The present study found no evidence of nasal carriage of S. aureus strains of poultry biotype by humans. The finding of hand carriage by broiler parent farm personnel, suggests that handling by personnel may contribute to the dissemination of I. 24 or other S. aureus strains associated with skeletal disease in broilers.     

The prediction of the intake potential of grass silage in the supplemented diets of lactating dairy cows

This study was undertaken to develop models which could be used in conjunction with the near infrared reflectance spectroscopy (NIRS) analysis of grass silage to accurately predict the intake potential of grass silage when offered to lactating dairy cows as part of a mixed diet. Empirical models were developed with data collected from two large-scale studies carried out at the Institute. The models comprised of (1) a linear equation for converting the NIRS-based predicted intake of a given silage for beef cattle to dairy cows and (2) a model which corrected the intake potential of the grass silage for supplementary concentrates. Furthermore, a milk yield adjustment factor of 0.14 kg DM/kg milk was utilised to standardise milk yields. Both linear and exponential models were developed to describe the decrease in silage intake as concentrate intake increased, with y-axis intercepts corresponding to unsupplemented silage intakes (NIRS-based predictions for beef cattle adjusted for dairy cows) and common x-axis intercept of 168.0 (SE=20.50) and 203.8 (SE=5.64) g/kg W^0.75, respectively, corresponding to concentrate intake when offered as a sole feed. A common r parameter (model curvature) of 1.0047 (SE=0.00397) was assumed for the exponential model. When the models were validated against the data from an independent study, the predictions from the two models were not significantly different, giving R² values of 0.70. The intercept and slope from the linear model were 5.39 and 1.01, respectively, and the intercept and slope from the exponential model were 6.10 and 0.98, respectively. Both intercepts and slopes were not significantly different from 0 and 1, respectively. Ninety-three percent of predictions were within 10% of observed intakes in the validation data.