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P. A. Massey R. E. Creamer R. P. O. Schulte M. J. Whelan K. Ritz 《Biology and Fertility of Soils》2013,49(8):1189-1201
The application of phosphorus (P)-based fertiliser to agricultural soils can result in a skewed vertical distribution of P down the soil profile, since the element tends to accumulate at the soil surface. Such accumulation can have detrimental effects on the environment, as the erosion of surface soil can facilitate the transfer of large quantities of P out of the field into water bodies. Earthworm and plant communities are intricately linked to vertical nutrient distributions in soil, with both communities either facilitating or negating the incorporation of nutrients into the soil matrix. This study aimed to investigate the effect of earthworm presence, plant community structure and fertiliser type to affect the distribution of nutrients in experimental mesocosms; with particular emphasis on the vertical distribution of P. Mesocosms were designed to simulate a soil which has a high P concentration at the surface compared to further down the soil profile. It was hypothesised that the presence of earthworms would facilitate the incorporation of P into the soil matrix and the presence of a more botanically diversity community would assimilate a greater quantity of soil nutrients. After 1 year, mesocosms were deconstructed into five depth ranges, and the distribution of nutrients were modelled and analysed. Results indicated that the presence of earthworms did redistribute soil nutrients from the surface into the matrix beneath. This redistribution was apparent from a reduction in nutrient concentrations in the 0–1 cm depth range. The presence of a more botanically diverse community not only assimilated greater soil nutrient concentrations, but also promoted a less even vertical distribution of soil nutrients, demonstrating the importance of soil biota and plant diversity in the redistribution of soil nutrients. 相似文献
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Objective Correlation of immunophenotype with history, anatomical and morphological features of lymphoid neoplasia in the koala.
Methods Routine necropsies were performed on 51 koalas with suspected lymphoid neoplasia between 1986 and 1997 in New South Wales and Queensland. Immuno-phenotyping was by an immunoperoxidase method utilising species cross-reactive antibodies raised against human lymphocytes and an antibody raised against koala IgG. Cases were classified according to organs and tissues affected and the morphological features of neoplastic cells.
Results Twenty-six (51%) of the cases were of the T cell immunophenotype, 12 (24%) were of B cell immunopheno-type and 13 (25%) did not stain. The age and sex of koalas did not correlate with immunophenotype (P = 0.686 and P = 1.000, respectively). Thirty-two cases were leukaemic and 36 had multiple organ involvement, probably reflecting presenta tion of koalas at advanced stages of disease. Abdominal tissue involvement was most common (44 cases), followed by nodal (32), atypical (21) and cervicomediastinal (14). The T cell immunophenotype was over-represented among the leukaemic cases (P = 0.013). Generally, the T cell immunophenotype predominated except for many affected atypical tissues. Neoplastic cells were mostly of medium nuclear size with round to oval nuclei. No correlations were found for cell morphology, mitotic index and immunopheno-type.
Conclusion The prognostic value of an immunopheno-typic, anatomical and morphological basis for the classifica tion of lymphoid neoplasia in the koala currently is limited by the need to detect these neoplasms at an early age, the requirement for freshly fixed tissues and the restricted range of available cross-reacting antibodies. 相似文献
Methods Routine necropsies were performed on 51 koalas with suspected lymphoid neoplasia between 1986 and 1997 in New South Wales and Queensland. Immuno-phenotyping was by an immunoperoxidase method utilising species cross-reactive antibodies raised against human lymphocytes and an antibody raised against koala IgG. Cases were classified according to organs and tissues affected and the morphological features of neoplastic cells.
Results Twenty-six (51%) of the cases were of the T cell immunophenotype, 12 (24%) were of B cell immunopheno-type and 13 (25%) did not stain. The age and sex of koalas did not correlate with immunophenotype (P = 0.686 and P = 1.000, respectively). Thirty-two cases were leukaemic and 36 had multiple organ involvement, probably reflecting presenta tion of koalas at advanced stages of disease. Abdominal tissue involvement was most common (44 cases), followed by nodal (32), atypical (21) and cervicomediastinal (14). The T cell immunophenotype was over-represented among the leukaemic cases (P = 0.013). Generally, the T cell immunophenotype predominated except for many affected atypical tissues. Neoplastic cells were mostly of medium nuclear size with round to oval nuclei. No correlations were found for cell morphology, mitotic index and immunopheno-type.
Conclusion The prognostic value of an immunopheno-typic, anatomical and morphological basis for the classifica tion of lymphoid neoplasia in the koala currently is limited by the need to detect these neoplasms at an early age, the requirement for freshly fixed tissues and the restricted range of available cross-reacting antibodies. 相似文献
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Objective To describe a case of diabetes mellitus in a koala (Phascolarctos cinereus).
Design A case report with controls.
Procedures We describe clinical and laboratory findings in a 6-year-old, free-living, female koala presented with traumatic injury and subsequently found to have polydipsia, hyperglycaemia and glucosuria. Over a 5 week period, serum biochemical analyses, haematological examinations, urinal-yses, measurement of serum insulin and fructosamine concentrations, necropsy, histopathological examination of a range of tissues and immunohistochemical examination of the pancreas for insulin-containing cells were done. For reference purposes, serum insulin and fructosamine concentrations were determined in four and two healthy koalas, respectively, and three healthy koalas pancreases were examined histo-logically and immunohistochemically.
Results The koala had persistent hyperglycaemia, hyperlipidaemia, hyponatraemia, hypochloraemia and glucosuria. Serum insulin concentration of the diabetic koala was only marginally smaller than that of healthy koalas, but all concentrations were smaller than reference concentrations in dogs and people. Fructosamine concentration did not allow the diabetic koala to be distinguished from healthy koalas and concentrations of all koala analytes were greater than expected for healthy dogs and people. Histopathological examination revealed extensive degeneration of pancreatic islet cells and fatty infiltration of hepatocytes. Immunoperoxidase staining revealed decreased or absent insulin in the b cells of the affected koala.
Conclusion Clinical signs, clinicopathological results and histopathological changes were consistent with diabetes mellitus. The pathogenesis of the condition could not be determined but may have been related to the administration of a parenteral corticosteroid preparation, the stress of capture or tissue damage and inflammation. 相似文献
Design A case report with controls.
Procedures We describe clinical and laboratory findings in a 6-year-old, free-living, female koala presented with traumatic injury and subsequently found to have polydipsia, hyperglycaemia and glucosuria. Over a 5 week period, serum biochemical analyses, haematological examinations, urinal-yses, measurement of serum insulin and fructosamine concentrations, necropsy, histopathological examination of a range of tissues and immunohistochemical examination of the pancreas for insulin-containing cells were done. For reference purposes, serum insulin and fructosamine concentrations were determined in four and two healthy koalas, respectively, and three healthy koalas pancreases were examined histo-logically and immunohistochemically.
Results The koala had persistent hyperglycaemia, hyperlipidaemia, hyponatraemia, hypochloraemia and glucosuria. Serum insulin concentration of the diabetic koala was only marginally smaller than that of healthy koalas, but all concentrations were smaller than reference concentrations in dogs and people. Fructosamine concentration did not allow the diabetic koala to be distinguished from healthy koalas and concentrations of all koala analytes were greater than expected for healthy dogs and people. Histopathological examination revealed extensive degeneration of pancreatic islet cells and fatty infiltration of hepatocytes. Immunoperoxidase staining revealed decreased or absent insulin in the b cells of the affected koala.
Conclusion Clinical signs, clinicopathological results and histopathological changes were consistent with diabetes mellitus. The pathogenesis of the condition could not be determined but may have been related to the administration of a parenteral corticosteroid preparation, the stress of capture or tissue damage and inflammation. 相似文献
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Insensitivity of soil biological communities to phosphorus fertilization in intensively managed grassland systems 下载免费PDF全文
Efficient soil P cycling is essential for promoting optimal and sustainable grassland growth. The soil biological community is regarded as an important source of available P to the plant community. However, the effects of P fertilization on the soil biota are unclear. This study aimed to investigate the effects of P fertilization on plant and soil biological communities in two intensively managed grassland sites that had been receiving mineral‐based P fertilization over a 14‐year period. Both pastures had been frequently cut and harvested for plant material and had received only inorganic fertilizers from the establishment of the trial. Both sites were sampled four times from October 2009 to April 2011 and plant and a range of soil biological parameters were determined at each sampling period. The main findings of this study showed that soil chemical measures, such as labile inorganic P and total P concentrations, and plant yield and P contents responded as expected to P fertilization. However, all soil biological parameters either showed no response or inconsistent responses to P fertilization over the experimental period. This study indicates that intensive management regimes, for example intensive plant harvesting and fertilizer regimes, appear to override the relationship between plant and soil biological communities with respect to their response to P fertilization, and thus their productivity is apparently not predicated upon biotic activity. 相似文献
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W E Massey 《Science (New York, N.Y.)》1989,245(4921):915-921
It is argued that the need to improve science education should be a national priority. Ways are suggested by which the federal government and the scientific community, working together, can address this issue. It is recommended that scientists, engineers, and educators make a significant personal and institutional commitment to participate in science education activities, and that the President of the United States provide the personal leadership to generate a national commitment to the improvement of education at all levels. 相似文献
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